TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|320165142|gb|EFW42041.1| sec tRNA synthase [Capsaspora
owczarzaki ATCC 30864]
         (470 letters)

Database: E.siliculosus/genome.fa 
           35 sequences; 195,934,119 total letters

Searching...................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genom...    93   2e-18

>emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
             assembly, chromosome LG17
          Length = 5807719

 Score = 93.2 bits (230), Expect = 2e-18
 Identities = 43/68 (63%), Positives = 56/68 (82%)
 Frame = +2

Query: 80    RVYSDLVARRHFRMAHGIGRSGDIAAIQPKAAGSSLIMKLVNIMCLDVIRLAGVHAANAC 139
             +VYS LVARR+FR++HG+GRSGDIA +QPKAAGSSL+ KL + +  D +RLAG+  A AC
Sbjct: 98831 QVYSGLVARRNFRLSHGVGRSGDIAEVQPKAAGSSLLAKLTHALAKDALRLAGLGNARAC 99010

Query: 140   IALPLATG 147
             + LP+ATG
Sbjct: 99011 LVLPMATG 99034



 Score = 60.8 bits (146), Expect = 1e-08
 Identities = 35/87 (40%), Positives = 49/87 (56%)
 Frame = +3

Query: 379    TLSTFGSDATRLGSFLFTRCVSGTRVVAADRAAATTSVGPVSLSNFGAHHSAYHGAYLTA 438
              T S   + A   GS LFTRCVSGTRVV  ++      VG +  + +GA  + Y   YLTA
Sbjct: 103188 TASGAPASAAFFGSMLFTRCVSGTRVVPREQHKC---VGGIDFNGYGASVTGYPHDYLTA 103358

Query: 439    AAAIGMTEQEVDALIDRLTAAFKDLRA 465
              A A G++ +E+D  + RL  A +  +A
Sbjct: 103359 ACAAGLSTEELDEFLIRLDKALRKAKA 103439



 Score = 59.3 bits (142), Expect = 4e-08
 Identities = 26/48 (54%), Positives = 35/48 (72%)
 Frame = +2

Query: 34    VKSLLAQRKWPELGWDEMSIELLLSDLAQMDSNNFPANIGAGEREARV 81
             V +LL+ R+ P  GWD+  IE LL++++ MDSNNF  N+G GERE RV
Sbjct: 98255 VTALLSNRRLPTRGWDDAQIEYLLTEISLMDSNNFSENVGVGEREGRV 98398



 Score = 55.1 bits (131), Expect = 7e-07
 Identities = 24/45 (53%), Positives = 35/45 (77%)
 Frame = +1

Query: 308    RIGEMYPGRASAAPSVDIFITLLSMGQQGFRSLLKQRKVVFEQLR 352
              ++G+ Y GRAS+ P +D+FITLLSMGQ+G+R LL +R+ + E  R
Sbjct: 101944 QVGKSYAGRASSGPCLDLFITLLSMGQEGWRRLLAERETLVEPFR 102078



 Score = 53.9 bits (128), Expect = 2e-06
 Identities = 21/41 (51%), Positives = 31/41 (75%)
 Frame = +2

Query: 198   GDEVRTDLSALQHQVNTVGKDNILCIVSTTSCFAPRCPDRI 238
             GD V TD+  ++  V + G +N+LC+V+TTSCFAPR PD++
Sbjct: 99773 GDAVATDVDGVRAAVESCGAENVLCVVTTTSCFAPRLPDKV 99895



 Score = 51.6 bits (122), Expect = 7e-06
 Identities = 23/54 (42%), Positives = 37/54 (68%)
 Frame = +3

Query: 144   LATGMAMVMTLLTLAQTRPPSARKVLWLRIDQKSCIKAITACGFEVVVVDNAIE 197
             L + M++ +T   L   +P +A+ ++W R+DQKSC+KAI A GF  +VV+N I+
Sbjct: 99366 LQSSMSLALTFAALRAAKP-AAKTIVWPRMDQKSCLKAIVAAGFTPIVVENLIQ 99524


  Database: E.siliculosus/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 195,934,119
  Number of sequences in database:  35
  
Lambda     K      H
   0.321    0.133    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 107,755,815
Number of Sequences: 35
Number of extensions: 1687162
Number of successful extensions: 9907
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 9597
Number of HSP's gapped (non-prelim): 414
length of query: 470
length of database: 65,311,373
effective HSP length: 117
effective length of query: 353
effective length of database: 65,307,278
effective search space: 23053469134
effective search space used: 23053469134
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 121 (51.2 bits)