TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= sp|Q9VKY8|SPS2_DROME Selenide, water dikinase 2 OS=Drosophila
melanogaster GN=Sps2 PE=2 SV=3
(370 letters)
Database: S.parasitica/genome.fa
1445 sequences; 53,132,636 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 102 5e-45
>gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.24, whole
genome shotgun sequence
Length = 471063
Score = 102 bits (254), Expect(4) = 5e-45
Identities = 72/196 (36%), Positives = 101/196 (51%), Gaps = 34/196 (17%)
Frame = +3
Query: 89 GRIALANVLSDVYAVGVTQFDTVEMIVSTSTSFSEKERDVVIGLVMKGFQNSLKANGYRN 148
GRIA ANVLSD+YA+GVT+ DT+ MI+ S + ++ ERDVV ++ GF + + G
Sbjct: 142827 GRIACANVLSDLYAMGVTEVDTMLMILGVSRTMTDLERDVVTTQLIHGFNDLAREAG--- 142997
Query: 149 TPLIIRQLKINPWCIIGGIA-----------------TSVCRSEEIILPSNAQPGDVLVL 191
T + Q +NPW I+GG+A T++ EII P NA G +LVL
Sbjct: 142998 TNVTGGQTVMNPWPIVGGVAMSVRAGSPMFSSTKRNLTNIHLESEIIRPENATAGQMLVL 143177
Query: 192 TKPLGGQMAM---------DAHLWQL--------NQTEKYKKLLSECSDADIKETFEIAV 234
TKPLG + + AH QL +Q ++ K+ S + F +A
Sbjct: 143178 TKPLGCALVLGGEFIASCCHAHSTQLAVNAYQMKDQPARWSKVESFLTREMADRAFSVAS 143357
Query: 235 KSMTYLNKNAALLMHK 250
+SM+ LN N A LMHK
Sbjct: 143358 ESMSRLNVNGAKLMHK 143405
Score = 57.8 bits (138), Expect(4) = 5e-45
Identities = 34/90 (37%), Positives = 47/90 (52%), Gaps = 19/90 (21%)
Frame = +2
Query: 256 ATDITGFGLLGHAN-------------------NLAQFQKEKVLFQINKLPIIKNVLKFS 296
ATD+TGFG+LGHA N+A QK V F+++ LP+IK++L +
Sbjct: 143471 ATDVTGFGILGHAQYGNTIGV*LLLTLAFAAARNMATSQKADVDFELHTLPVIKDMLAVN 143650
Query: 297 TLVGQSTKFRSGRSVETSGGLLICLPADAA 326
+ G S ETSGGLL+ + AD A
Sbjct: 143651 EAFNGQWRLELGFSSETSGGLLVSIDADRA 143740
Score = 43.5 bits (101), Expect(4) = 5e-45
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +1
Query: 2 FQPEKHGLEPDFQLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNND 48
F P HGL DF+LT F+ G CK+PQ+ L Y+ G + K N+
Sbjct: 142483 FNPTDHGLREDFRLTDFSALKG*GCKLPQEKLLSYISGIANDLKPNE 142623
Score = 37.7 bits (86), Expect(4) = 5e-45
Identities = 17/33 (51%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = +2
Query: 55 GMDCAVIPLKRHK--DYLLIQTVDFFYPMVNDP 85
GMD +V+ +K K D +I T DFFYP+V DP
Sbjct: 142676 GMDSSVVKIKHPKFGDLYMISTTDFFYPLVQDP 142774
Database: S.parasitica/genome.fa
Posted date: Feb 9, 2011 9:02 AM
Number of letters in database: 53,132,636
Number of sequences in database: 1445
Lambda K H
0.319 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,553,385
Number of Sequences: 1445
Number of extensions: 254271
Number of successful extensions: 1093
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 35
Number of HSP's that attempted gapping in prelim test: 880
Number of HSP's gapped (non-prelim): 414
length of query: 370
length of database: 17,710,878
effective HSP length: 105
effective length of query: 265
effective length of database: 17,559,153
effective search space: 4653175545
effective search space used: 4653175545
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 115 (48.9 bits)