TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= sp|Q9VKY8|SPS2_DROME Selenide, water dikinase 2 OS=Drosophila
melanogaster GN=Sps2 PE=2 SV=3
         (370 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    94   3e-19
gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    88   1e-17

>gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_8, whole genome shotgun
              sequence
          Length = 938756

 Score = 93.6 bits (231), Expect = 3e-19
 Identities = 105/373 (28%), Positives = 161/373 (43%), Gaps = 40/373 (10%)
 Frame = +1

Query: 2      FQPEKHGLEPDFQLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNND-GYLIGSGM--DC 58
              F  E  G   + +LT  T   G  CKI  + L   L  ++I  +  D   L+G+    DC
Sbjct: 697936 FSAENEGF--NVKLTSTTHGLGCGCKIRAQTLSSIL--SKIPKQPFDPNVLVGTETSDDC 698103

Query: 59     AVIPLKRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTS 118
               V  L    +  L  TVDFF P+V+ P+  G IA AN LSDVYA+G      + ++    
Sbjct: 698104 GVYKLT--SELALCATVDFFTPIVDSPKDFGAIACANALSDVYAMGGECKVALNIV---- 698265

Query: 119    TSFSEKERDV-VIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSEEI 177
               +F  K   + ++ L+++G Q   KA+    T +    ++ N     G   + +   E I
Sbjct: 698266 -AFPVKRLSLDILQLILQGAQE--KASEAGVTIIGGHSIEDNE-PKFGMCVSGLVHPERI 698433

Query: 178    ILPSNAQPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADIKETFEIAVKSM 237
                    + GDVL+LTKP+G  +           T   K L+    D   K      ++ M
Sbjct: 698434 WKNVGCRNGDVLLLTKPIGTGVL---------TTAMKKGLIGPNDDCSKK-----CIEWM 698571

Query: 238    TYLNKNAALLMH----------KYQAHCATDITGFGLLGHA----------NNLAQFQKE 277
              + LNK+A+ ++            Y+ H  TDITGFGL+GH           N  AQ + E
Sbjct: 698572 STLNKSASEILRGLHRVNSSSTPYEIHACTDITGFGLMGHLREMITGDNQDNLYAQIESE 698751

Query: 278    KVLFQ----------------INKLPIIKNVLKFSTLVGQSTKFRSGRSVETSGGLLICL 321
              KV F                 IN +  ++ + KF+  V Q  +       +TSGGLL  +
Sbjct: 698752 KVPFMDKAYEMILSDIIPGGTINNMNFVQKITKFNESVSQIVRTLL-CDAQTSGGLLFSV 698928

Query: 322    PADAADKFCRDFE 334
                  +++   DF+
Sbjct: 698929 SPHESEEIISDFK 698967


>gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_39, whole genome
             shotgun sequence
          Length = 371401

 Score = 87.8 bits (216), Expect = 1e-17
 Identities = 89/355 (25%), Positives = 154/355 (43%), Gaps = 33/355 (9%)
 Frame = -2

Query: 14    QLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNNDGYLIGSGM--DCAVIPLKRHKDYLL 71
             +LT+  +  G  CKI  ++L+  L+    + +NN   L+G+    D AV  +  H    +
Sbjct: 75705 KLTQLASGLGCGCKINSRLLDSILQKLPKQPENNS-VLVGNSTCDDAAVYKINDH--VAI 75535

Query: 72    IQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTSTSFSEKERDVVIG 131
             + T+DFF P+V++P   G IA AN LSDVYA+G      + ++   S       R + I 
Sbjct: 75534 VSTIDFFAPIVDNPYDFGAIACANALSDVYAMGGKPQTALSVVAFPS-------RTLPIE 75376

Query: 132   LVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSEEIILPSNAQPGDVLVL 191
             ++ +  + S+         ++      +     G     +   ++I      + GD+L L
Sbjct: 75375 ILQEILRGSIDKANEAGVAIVGGHSIDDEEPKFGMSVNGIIHPDKIFRNVGCEEGDLLFL 75196

Query: 192   TKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADIKETFEIAVKSMTYLNKNAALLMHKY 251
             TK +G  +        +    K   +  E       ET + A+ SM  LN++ + ++ + 
Sbjct: 75195 TKRIGTGV--------ITSAMKLGNVPYE------SETAQSAINSMKTLNRDTSYVLREM 75058

Query: 252   QA-HCATDITGFGLLGHANNLAQFQKE------KVLFQINK----------LPIIKNVLK 294
             ++    TD+TGFGLLGH   + +  +E       ++ Q+N           L +++ V+ 
Sbjct: 75057 KSVSSCTDVTGFGLLGHLKEIMKGSREICEENKDLVAQLNAKQIPLLDSSCLSLMRIVIP 74878

Query: 295   FSTL-----VGQSTKFRSGRSVE---------TSGGLLICLPADAADKFCRDFEE 335
               T      V Q  +F S  S E         TSGGLL  +  D  +K    F+E
Sbjct: 74877 GGTKTNLENVQQFVEFASTLSKEEQLILADAQTSGGLLFTISPDEEEKLVNSFKE 74713


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,083,619
Number of Sequences: 784
Number of extensions: 207419
Number of successful extensions: 942
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 785
Number of HSP's gapped (non-prelim): 372
length of query: 370
length of database: 13,654,695
effective HSP length: 103
effective length of query: 267
effective length of database: 13,573,943
effective search space: 3624242781
effective search space used: 3624242781
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 114 (48.5 bits)