TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|40787830|gb|AAH65165.1| Selenophosphate synthetase 1 [Mus
musculus]
         (392 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   115   9e-26
gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   104   2e-22

>gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_8, whole genome shotgun
              sequence
          Length = 938756

 Score =  115 bits (287), Expect = 9e-26
 Identities = 98/372 (26%), Positives = 164/372 (44%), Gaps = 31/372 (8%)
 Frame = +1

Query: 19     RLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDT---CVIPL 75
              +LT  T   G GCK+    L  +L  + +  F           P + +G +T   C +  
Sbjct: 697966 KLTSTTHGLGCGCKIRAQTLSSILSKIPKQPFD----------PNVLVGTETSDDCGVYK 698115

Query: 76     RHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDR 135
                  L+L  T D+  PIVD P   G IACAN LSD+YAMG  EC   L ++    K    
Sbjct: 698116 LTSELALCATVDFFTPIVDSPKDFGAIACANALSDVYAMG-GECKVALNIVAFPVKRLSL 698292

Query: 136    ERDKVIPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGD 195
              +   ++ LI+QG ++ A EAG ++ GG ++ +     G   + +  P           GD
Sbjct: 698293 D---ILQLILQGAQEKASEAGVTIIGGHSIEDNEPKFGMCVSGLVHPERIWKNVGCRNGD 698463

Query: 196    VLVLTKPLGTQV-AVAVHQWLDIPEKWNKIKLVVTQEDVELVYQEAMMNMARLNRTAAGL 254
              VL+LTKP+GT V   A+ + L  P      K +     +     E +  + R+N ++   
Sbjct: 698464 VLLLTKPIGTGVLTTAMKKGLIGPNDDCSKKCIEWMSTLNKSASEILRGLHRVNSSST-- 698637

Query: 255    MHTFNAHAATDITGFGILGHAQNL----------AKQQRNEVSFV--------------- 289
                 +  HA TDITGFG++GH + +          A+ +  +V F+               
Sbjct: 698638 --PYEIHACTDITGFGLMGHLREMITGDNQDNLYAQIESEKVPFMDKAYEMILSDIIPGG 698811

Query: 290    -IHNLPVLAKMAAVSKACGNMFGLMHGTC-PETSGGLLICLPREQAARFCAEIKSPKYGE 347
               I+N+  + K+   +++   +   +   C  +TSGGLL  +   ++    ++ K      
Sbjct: 698812 TINNMNFVQKITKFNESVSQIVRTL--LCDAQTSGGLLFSVSPHESEEIISDFKR----L 698973

Query: 348    GHQAWIIGIVEK 359
              G   W +G +++
Sbjct: 698974 GISIWTVGSIKQ 699009


>gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_39, whole genome
             shotgun sequence
          Length = 371401

 Score =  104 bits (259), Expect = 2e-22
 Identities = 78/269 (28%), Positives = 128/269 (47%), Gaps = 4/269 (1%)
 Frame = -2

Query: 19    RLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTC---VIPL 75
             +LT+     G GCK+   +L  +L+ L +    E+   L        +G  TC    +  
Sbjct: 75705 KLTQLASGLGCGCKINSRLLDSILQKLPKQ--PENNSVL--------VGNSTCDDAAVYK 75556

Query: 76    RHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDR 135
              +  +++V T D+  PIVD+PY  G IACAN LSD+YAMG  +    L ++   ++    
Sbjct: 75555 INDHVAIVSTIDFFAPIVDNPYDFGAIACANALSDVYAMG-GKPQTALSVVAFPSRTLPI 75379

Query: 136   ERDKVIPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGD 195
             E   ++  I++G  D A EAG ++ GG ++ +     G     +  P++         GD
Sbjct: 75378 E---ILQEILRGSIDKANEAGVAIVGGHSIDDEEPKFGMSVNGIIHPDKIFRNVGCEEGD 75208

Query: 196   VLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELVYQEAMMNMARLNR-TAAGL 254
             +L LTK +GT V  +  +  ++P +                 Q A+ +M  LNR T+  L
Sbjct: 75207 LLFLTKRIGTGVITSAMKLGNVPYE-------------SETAQSAINSMKTLNRDTSYVL 75067

Query: 255   MHTFNAHAATDITGFGILGHAQNLAKQQR 283
                 +  + TD+TGFG+LGH + + K  R
Sbjct: 75066 REMKSVSSCTDVTGFGLLGHLKEIMKGSR 74980


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.320    0.136    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,140,614
Number of Sequences: 784
Number of extensions: 226094
Number of successful extensions: 714
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 630
Number of HSP's gapped (non-prelim): 174
length of query: 392
length of database: 13,654,695
effective HSP length: 104
effective length of query: 288
effective length of database: 13,573,159
effective search space: 3909069792
effective search space used: 3909069792
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 114 (48.5 bits)