TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|198421765|ref|XP_002125303.1| PREDICTED: similar to tRNA
selenocysteine-associated protein 1 (SECp43) [Ciona intestinalis]
         (324 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   116   2e-26
gb|GG738891.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    65   1e-10
gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    56   4e-08
gb|GG738877.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    51   2e-06

>gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun
              sequence
          Length = 707703

 Score =  116 bits (291), Expect = 2e-26
 Identities = 77/190 (40%), Positives = 110/190 (57%), Gaps = 18/190 (9%)
 Frame = -1

Query: 2      SRSLWMGDLEPYMDETFVNKAFLQVSQPV--SVKVIRRKDNGLPAGYCFIEFPSEAEAER 59
              +R+LWMGDL+  MD+ +V K F    +P   SVK+I+ ++ G   GY F+EF S   A+ 
Sbjct: 463674 ARTLWMGDLDDSMDDEYVKKLFANDIRPSINSVKIIKDRNTGKSIGYGFVEFASIEIAKA 463495

Query: 60     VLKLVNGTTINGSNPPKRFRLN------------RSQAGKMWDIGPS---FSIFVGDLDA 104
              VL+   G  I  + P K +RLN             SQ G     G      SIFVGDL  
Sbjct: 463494 VLESYAGKPIP-TLPNKIYRLNWAAQNQGSNPLFSSQPGGKPSSGGKENIVSIFVGDLAP 463318

Query: 105    TVTDDKLEDFFLKRYRSVKGAKIMYE-EGGISRGYGFVRFSDEAEQKRALQEMQGIRGLG 163
               V D  LE  F  RY SV+GAK++ + + GIS+GYGFV+F+DE +  R++ EMQG+  + 
Sbjct: 463317 DVNDYMLEQTFRNRYPSVRGAKVVMDPKSGISKGYGFVKFADEDDMMRSMTEMQGVY-IS 463141

Query: 164    AKPIRVSVAT 173
              ++P+++S AT
Sbjct: 463140 SRPVKISHAT 463111


>gb|GG738891.1| Naegleria gruberi genomic scaffold NAEGRscaffold_47, whole genome shotgun
              sequence
          Length = 339217

 Score = 64.7 bits (156), Expect = 1e-10
 Identities = 45/161 (27%), Positives = 84/161 (52%), Gaps = 8/161 (4%)
 Frame = -2

Query: 4      SLWMGDLEPYMDETFVNKAFLQVSQPVSVKVI---RRKDNG----LPAGYCFIEFPSEAE 56
              ++++ +L+  +D   +   F      +S KV    ++ DNG      AGY F+ F ++  
Sbjct: 283398 NIFIKNLDKSIDNKALYDTFSAFGNILSCKVCQATKKGDNGGDNVESAGYGFVHFETQEA 283219

Query: 57     AERVLKLVNGTTINGSNPPKRFRLNRSQAGKMWDIGPSFS-IFVGDLDATVTDDKLEDFF 115
              AE+ +  +NG  +NG        + +++  K+     SF+ I+V +LDA+V + +L + F
Sbjct: 283218 AEKAIAKINGMLLNGKQVFVGPFVKKTERLKILSNEDSFTNIYVKNLDASVDEKELSEVF 283039

Query: 116    LKRYRSVKGAKIMYEEGGISRGYGFVRFSDEAEQKRALQEM 156
               K +  ++ A +M  E G S+ +GF+ F+D A    A+ EM
Sbjct: 283038 SK-FGEIQNAVVMRGENGASKEFGFINFADHASALIAIDEM 282919



 Score = 58.5 bits (140), Expect = 8e-09
 Identities = 43/166 (25%), Positives = 80/166 (48%), Gaps = 8/166 (4%)
 Frame = -2

Query: 2      SRSLWMGDLEPYMDETFVNKAFLQVSQPVSVKVIRRKDNGLPAGYCFIEFPSEAEAERVL 61
              S SL++GDL   + E  +   F +V   +S+++ R        GY ++ F +  +AER L
Sbjct: 283668 SASLYVGDLHEEISEALLFDLFHEVGPIISIRICRDAMTRKSLGYAYVNFQNPQDAERAL 283489

Query: 62     KLVNGTTINGSNPPKRFRLNRSQAGKMWDIGPSFSIFVGDLDATVTDDKLEDFFLKRYRS 121
                +N  ++ G  P +     R  + +   IG   +IF+ +LD ++ +  L D F   + +
Sbjct: 283488 DTLNYASVKGI-PIRIMWSQRDPSIRKSGIG---NIFIKNLDKSIDNKALYDTF-SAFGN 283324

Query: 122    VKGAKIMY-----EEGG---ISRGYGFVRFSDEAEQKRALQEMQGI 159
              +   K+       + GG    S GYGFV F  +   ++A+ ++ G+
Sbjct: 283323 ILSCKVCQATKKGDNGGDNVESAGYGFVHFETQEAAEKAIAKINGM 283186



 Score = 53.9 bits (128), Expect = 2e-07
 Identities = 48/184 (26%), Positives = 86/184 (46%), Gaps = 12/184 (6%)
 Frame = -2

Query: 4      SLWMGDLEPYMDETFVNKAFLQVSQPVSVKVIRRKDNGLPAGYCFIEFPSEAEAERVLKL 63
              ++++ +L+  +DE  +++ F +  + +   V+ R +NG    + FI F   A A   +  
Sbjct: 283098 NIYVKNLDASVDEKELSEVFSKFGE-IQNAVVMRGENGASKEFGFINFADHASALIAIDE 282922

Query: 64     VNGTTINGSN---------PPKRFRLNRS-QAGKMWDIGP--SFSIFVGDLDATVTDDKL 111
              +N                   +R +L    Q  K   +      +++V +LD +V D++L
Sbjct: 282921 MNEKDFKNKKLFVGRAQKKNERRAKLKEYFQKLKQEKVNKYKGLNLYVKNLDDSVDDERL 282742

Query: 112    EDFFLKRYRSVKGAKIMYEEGGISRGYGFVRFSDEAEQKRALQEMQGIRGLGAKPIRVSV 171
                 F K +  +  AK+M  +   SRG+GFV F       +AL EM G   +G+KP+ V+ 
Sbjct: 282741 RQEFSK-FGDITSAKVM-SDNKQSRGFGFVCFKTPEAANKALTEMSG-HMIGSKPLYVNF 282571

Query: 172    ATPK 175
              A PK
Sbjct: 282570 AQPK 282559


>gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_3, whole genome shotgun
              sequence
          Length = 941567

 Score = 56.2 bits (134), Expect = 4e-08
 Identities = 54/228 (23%), Positives = 101/228 (44%), Gaps = 8/228 (3%)
 Frame = +3

Query: 4      SLWMGDLEPYMDETFVNKAFLQVSQPVSVKVIRRKDNGLPAGYCFIEFPSEAEAERVLKL 63
              ++++G+L+  +DE  + + F+Q+   V  K+ R +     +GY F+EF  E +A   +++
Sbjct: 787728 TIFVGNLDQQVDEELLWELFVQMGPVVDAKIPRDRITNTHSGYAFVEFKHEHDANYAIQV 787907

Query: 64     VNGTTINGSNPPKRFRLNRSQAGKMWDIGPSFSIFVGDLDATVTDDK--LEDFFLKRYRS 121
              +N   + G  P K  R ++ ++ K  D+G   +++VG+LD     D+  L + F +    
Sbjct: 787908 MNQIKLFG-RPMKLNRYDQDKSAKNLDVGA--NLWVGNLDPVGVSDEGILRELFGQFGVM 788078

Query: 122    VKGAKIMYE--EGGISRGYGFVRFSDEAEQKRALQEMQGIRGLGAKPIRVSVA-TPKGKM 178
              ++    +    E   S+G+ FV + +      A   + G + +  KPI V  A  P  + 
Sbjct: 788079 IQNTPRIQRDPETMESKGFAFVSYDNFESADAAKMHLNG-QYISGKPIIVEYAFKPNSRE 788255

Query: 179    APSTNSSTTDTTTASIPTAYDDATAYAQQLLQMQQ---YTDQYKYYQQ 223
                 + +         I            Q LQ QQ   Y  Q +  QQ
Sbjct: 788256 RYGSEAERELAAKRPITAQQQQMRLNHMQQLQAQQMLAYQAQLQQVQQ 788399


>gb|GG738877.1| Naegleria gruberi genomic scaffold NAEGRscaffold_33, whole genome
             shotgun sequence
          Length = 394700

 Score = 50.8 bits (120), Expect = 2e-06
 Identities = 41/131 (31%), Positives = 59/131 (45%), Gaps = 7/131 (5%)
 Frame = -2

Query: 93    PSF---SIFVGDLDATVTDDKLEDFFLK---RYRSVKGAKIMYEEGGISRGYGFVRFSDE 146
             PSF    IFVG L  T ++ +L DFF +    Y  +    IM +  G SRG+GFV FSDE
Sbjct: 56683 PSFINRRIFVGGLKETTSEQELIDFFSRSPNNYGPISHCVIMKKWDGRSRGFGFVTFSDE 56504

Query: 147   AEQKRALQ-EMQGIRGLGAKPIRVSVATPKGKMAPSTNSSTTDTTTASIPTAYDDATAYA 205
             +  K+ L    +    +G   I   VA  +G    S +  T  +T +S     DD   + 
Sbjct: 56503 SIAKKVLSYHKEHPFMIGNHRIDCQVAQSQGCKIDSVDIPTHASTISSSSRMVDDERLFT 56324

Query: 206   QQLLQMQQYTD 216
               +      +D
Sbjct: 56323 NNIHNSDTQSD 56291


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.315    0.130    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,119,973
Number of Sequences: 784
Number of extensions: 176813
Number of successful extensions: 2267
Number of sequences better than 1.0e-05: 4
Number of HSP's better than  0.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 86
Number of HSP's that attempted gapping in prelim test: 1099
Number of HSP's gapped (non-prelim): 1722
length of query: 324
length of database: 13,654,695
effective HSP length: 102
effective length of query: 222
effective length of database: 13,574,727
effective search space: 3013589394
effective search space used: 3013589394
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 113 (48.1 bits)