TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000037_1.0 # Protein # SECIS binding protein 2 (SBP2) #
Homo sapiens # Complete
         (854 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   120   6e-27
gb|GG738874.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    53   1e-06

>gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_1, whole genome shotgun
              sequence
          Length = 1555553

 Score =  120 bits (301), Expect = 6e-27
 Identities = 80/252 (31%), Positives = 135/252 (53%), Gaps = 9/252 (3%)
 Frame = +2

Query: 523    IPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTSDDTQDGESGGDDQFPEQAELS-GP 581
              + K KKP  LKK+ILKE++ RK     + ++P    D  +  ++  +D   E   +   P
Sbjct: 898847 LTKKKKPNKLKKLILKEKEIRKL----HNIAPGEFLDVKKFLKNLKEDTETESVVIEIVP 899014

Query: 582    EGMDE---LISTPSVEDKSEEPPGTE--LQRDTEASHLA---PNHTTFPKIHSRRFRDYC 633
              E  +E   L ++ ++  K ++    E  L  D E   L+            + R+ R+Y 
Sbjct: 899015 EKTEETTILENSKTILTKEDDSSDGEEKLVADDEMDILSIAESMEVVLKNSNERKIREYV 899194

Query: 634    SQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVI 693
              +  L  + +  +  +L +L  +Q R+ + D  K K + R V+G+REVLK LKLK ++ V+
Sbjct: 899195 NYPLDPDYENIIFQMLTKLQFYQFRLKKTDKKKFKQRLRYVIGIREVLKGLKLKHIRAVV 899374

Query: 694    ISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYD 753
              I+PN +K+ + GGLD  +  II    EQ +P  FAL +K LG ++  +  +S+VG++S D
Sbjct: 899375 IAPNIDKVTTPGGLDTKIEEIIQKCNEQELPVFFALTKKKLGTAMKTSTKISLVGLYSMD 899554

Query: 754    GAQDQFHKMVEL 765
              GA D+F K  ++
Sbjct: 899555 GAFDEFKKAKQI 899590


>gb|GG738874.1| Naegleria gruberi genomic scaffold NAEGRscaffold_30, whole genome shotgun
              sequence
          Length = 401612

 Score = 53.1 bits (126), Expect = 1e-06
 Identities = 112/551 (20%), Positives = 201/551 (36%), Gaps = 39/551 (7%)
 Frame = +2

Query: 108    GSQYLYNQPSCYRGFQTVKHRNENTCPLPQEMKA----LFKKKTYDEKKTYDQQKFDSER 163
              G +     PS  +  ++ K  +E   P     K      F  K   EKK     +F + +
Sbjct: 326201 GKRKKQTSPSTKKEAKSKKADDEEMKPKKNSTKVESTQSFDSKPVVEKKISPVTRFITTK 326380

Query: 164    ADGTISSEIKSARGSHHLSIYAENSLKSDGYHKRTDRKSRIIAKNVS--TSKPEFEFTTL 221
              A  T S +    +    +    +NS  +    K+  +KS+   +  S  + K   +  + 
Sbjct: 326381 AVSTKSDQETKPKKKKEVEAPKKNSKTTSEKSKKQSKKSKKQVEQTSEKSGKKNTKKVSK 326560

Query: 222    DFPELQGAENNMSEIQK-QPKWGPV-------HSVSTDISLLREVVKPAAVLSKGEIVVK 273
                 E+  +    S+I++ +PK G           VS  +    +      V S+ +I  K
Sbjct: 326561 KTTEISDSSKKTSDIKETKPKKGKKLVKDSSEKKVSKSVKSSMKTKSKKEVDSQQDIPKK 326740

Query: 274    NNPNE-------SVTANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMIN-- 324
               +  +       ++++ +  +SP+  +E             +S E     K   S I+  
Sbjct: 326741 TSEGKETKPKKSAISSKSVDSSPTIKQETKPKKGSSGKNGNVSNETPQKSKKEDSKISYQ 326920

Query: 325    ----------LKTIASSADPKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGSDLEQ 374
                        +K  AS++  K+ S   + A S   S   E  I  P++KSK  +  DL  
Sbjct: 326921 EKKQKKKVKKVKKGASTSSKKSTS--ETSAKSKKDSSRNEDEISIPSKKSKGGKKKDLTS 327094

Query: 375    N-EASRKNKKKKEKSTSKYEVLTVQEPPRIEDAEEFPNLAVASERRDRIETPKFQSKQQP 433
              + +A++K K KKE+  S  E  T+Q   +        N +V        ETPK     + 
Sbjct: 327095 SVDAAKKQKSKKEQVQSNDESTTIQNKKKKRKDTSTSNSSV--------ETPKKDKASKK 327250

Query: 434    QDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQSSKPVVVSVGAVPVLSKECASGE 493
                N ++   K ++P++           + Q ++     SK        +P + K   S E
Sbjct: 327251 NANSEDEAPKLRIPIRKS--------RQAQGNEETPIQSKKSKGGKKKIPKVDKSNKS-E 327403

Query: 494    RGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVS 553
              + +   +++ P      S          E+PK + PT             +    +NA  
Sbjct: 327404 KSQDKPKLRIPSRKTSPSR------DDEEVPKLRIPT-----------RGQSNSPKNAPK 327532

Query: 554    PAFTSDDTQDGESG-----GDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRD 608
               + TS   Q  ESG       D  P   E +        ISTP V   +++   ++ Q  
Sbjct: 327533 QSETSSQ-QRIESGMMTIKSVDSVPVTVERT----QKNTISTPKVRIPTKKGKDSKKQPK 327697

Query: 609    TEASHLAPNHT 619
              TE+   + N +
Sbjct: 327698 TESISSSQNES 327730


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.310    0.127    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,915,228
Number of Sequences: 784
Number of extensions: 443392
Number of successful extensions: 2223
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 87
Number of HSP's successfully gapped in prelim test: 113
Number of HSP's that attempted gapping in prelim test: 1340
Number of HSP's gapped (non-prelim): 1518
length of query: 854
length of database: 13,654,695
effective HSP length: 111
effective length of query: 743
effective length of database: 13,567,671
effective search space: 10080779553
effective search space used: 10080779553
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 118 (50.1 bits)