TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|117167838|gb|AAI15504.2| Pstk protein [Mus musculus]
         (295 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    68   9e-12

>gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_5, whole genome shotgun
              sequence
          Length = 850158

 Score = 68.2 bits (165), Expect = 9e-12
 Identities = 38/145 (26%), Positives = 80/145 (55%), Gaps = 3/145 (2%)
 Frame = -1

Query: 149    LVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCPLETCLKRNGERSQPLPDETIQLMGR 208
              ++LDDNFYY+SMR+E  Q+ +++++   Q+ L C +ETC++R+ +RSQP+ ++ I+ M  
Sbjct: 122172 IILDDNFYYKSMRHEFVQICQEFTIAHIQIILKCNVETCIERDAKRSQPVGEDIIRSMNI 121993

Query: 209    KIEKPNPEKNAWEHNSLIIQSSACSLEASLEVTGLL--LTALENPIKCVED-NTEQKETD 265
              + E     K+++  N + ++    +      ++  +  L  L  P++ +     E KE  
Sbjct: 121992 RFEY---GKSSFSDNIIYMEYDTDNFSEMDSISSKIIDLFPLAKPLQDLNKMKQEMKEES 121822

Query: 266    RIICSTNILHKADETLRRTVSQTMR 290
              +     +   + D++LR+  S  ++
Sbjct: 121821 QRQNEQSFKKQLDDSLRKKTSHLIQ 121747


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.320    0.134    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,812,060
Number of Sequences: 784
Number of extensions: 208572
Number of successful extensions: 919
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 762
Number of HSP's gapped (non-prelim): 352
length of query: 295
length of database: 13,654,695
effective HSP length: 101
effective length of query: 194
effective length of database: 13,575,511
effective search space: 2633649134
effective search space used: 2633649134
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 113 (48.1 bits)