TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= EhSep2_emiliania_huxley; gi|182639182|sp|Q50KB1.2|SEP2_EMIHU
RecName: Full=Protein disulfide-isomerase-like protein EhSep2; Flags:
Precursor # QUERY
         (223 letters)

Database: P.tricornutum/genome.fa 
           31 sequences; 23,733,684 total letters

Searching...............................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...   133   7e-32
gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    73   1e-13
gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    71   4e-13
gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    47   8e-06

>gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun
              sequence
          Length = 2535400

 Score =  133 bits (335), Expect = 7e-32
 Identities = 73/177 (41%), Positives = 111/177 (62%), Gaps = 1/177 (0%)
 Frame = +1

Query: 48     GHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGE 107
              GHCKKM PDW+ LA  ++     LIA+VDCTT GKPLC+  GVRG+PT+KY +P     E
Sbjct: 450259 GHCKKMAPDWEKLAEEWDGHAVGLIAEVDCTTEGKPLCDANGVRGFPTLKYGDPAGL--E 450432

Query: 108    DYKGGRSLDELKKFAENELGPGCSVDLMDNCSEEQKGKLKEYIDMAPEKRTEMLETLKKE 167
              DY+G RS D+L  FA+  L P CS   +D C +E+K ++++Y+ ++ +     ++  +++
Sbjct: 450433 DYQGSRSFDDLATFAKENLKPVCSPANLDLCDDEKKKQIEDYMALSDDDLESKIKAEEEK 450612

Query: 168    LADAESTHEALLKELQATY-KESMDKLEKLKEESAPKIKLLKAATPAPKAEGAKDEV 223
              L  AE+  +  + +LQA Y K S DK EK+       + LLK +  A K++ AK+E+
Sbjct: 450613 LEAAEAEFKEAVTKLQADYQKLSEDKDEKIAAVKNSGLGLLK-SVKASKSKTAKEEL 450780



 Score = 86.3 bits (212), Expect = 1e-17
 Identities = 56/181 (30%), Positives = 100/181 (55%), Gaps = 5/181 (2%)
 Frame = -2

Query: 48     GHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGE 107
              GHCK M  DW+ LA  ++D +  L+A+VDCT   + LCE+  V G+PT+ Y +      E
Sbjct: 956097 GHCKAMAEDWEELAEIWKDHEVGLVAEVDCTV-EEQLCEELEVTGFPTLMYGDAMSL--E 955927

Query: 108    DYKGGRSLDELKKFAENELG-PGCSVDLMDNCSEEQKGKLKEYIDMAPEKRTEMLETLKK 166
               Y+G R L+ LK FAE  +  P CSV  +D+C +E +  + +    + E+  E    +  
Sbjct: 955926 LYEGPRDLETLKAFAEENIAKPICSVRNLDSCGDEVQKVIADLQTKSREELEEAAAAVDA 955747

Query: 167    ELADAESTHEALLKELQATYKESM----DKLEKLKEESAPKIKLLKAATPAPKAEGAKDE 222
              +L  +++  +  L+E+   Y++ +    +K++++ E+S  K  + +  +     EGA+DE
Sbjct: 955746 KLEASQAEFDEGLEEINDQYEKMVESYNEKVDEIHEKSNYK-WVKQILSSFEDEEGAEDE 955570

Query: 223    V 223
              +
Sbjct: 955569 L 955567



 Score = 64.7 bits (156), Expect = 4e-11
 Identities = 41/109 (37%), Positives = 57/109 (52%)
 Frame = -2

Query: 40      IKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYF 99
               +KF APW GHCK++ P  D +A   E + K+ I  VDCT   K LC ++ V+ YPT+K+ 
Sbjct: 1104927 LKFYAPWCGHCKRLAPILDQVAP--EIAGKMAIGKVDCTQ-EKKLCNQHNVKSYPTLKF- 1104760

Query: 100     NPPDEEGEDYKGGRSLDELKKFAENELGPGCSVDLMDNCSEEQKGKLKE 148
                  D    DY GGR   ++  FA     P   V +MD+     +   KE
Sbjct: 1104759 -SLDGHLFDYPGGRKEGDIIAFANKINRP--HVQMMDSMDSVMEYLYKE 1104622



 Score = 47.0 bits (110), Expect = 8e-06
 Identities = 30/103 (29%), Positives = 50/103 (48%), Gaps = 25/103 (24%)
 Frame = -2

Query: 38      AFIKFLAPWUGHCKKMKPDWDSLAST--------------------FEDSKK----VLIA 73
               +F++F      HC+ + P W++LA                      +E +KK    V+IA
Sbjct: 2054670 SFVRFRC---SHCRALAPTWETLAEVMSDVAEDLVEQHDHEYSEEEYEHAKKVEMPVMIA 2054500

Query: 74      DVDCTTGGKPLCEKYGVRGYPTIKYFNPPDE-EGEDYKGGRSL 115
                +DC    K +C K G+ GYPT++ F   +  +G DY+G R++
Sbjct: 2054499 KIDCVL-HKQVCMKQGILGYPTLRLFVDGERWKGGDYRGDRTV 2054374


>gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 13, whole genome shotgun
              sequence
          Length = 887524

 Score = 73.2 bits (178), Expect = 1e-13
 Identities = 54/145 (37%), Positives = 73/145 (50%), Gaps = 9/145 (6%)
 Frame = +1

Query: 50     CKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDY 109
              C +MKPDWD LA   E    V IADV+C+   + LCEK  V GYPTIK +   D   E Y
Sbjct: 242044 CTRMKPDWDKLAE--EAHSSVFIADVNCSDQAE-LCEKNDVAGYPTIKVYK--DGVDEKY 242208

Query: 110    KGGRSLDELKKFAENELGPGCSVD-LMDNCSEE-----QKGKLKEYIDMAPE-KRTEML- 161
               G R  D+L  + +  L   C V  L + C E+      K K +E  D+  E  R E + 
Sbjct: 242209 NGARGFDDLMDYVDANLASKCDVSKLEETCGEKAVKYANKWKDREAADVTKEFSRLEGMA 242388

Query: 162    -ETLKKELADAESTHEALLKELQAT 185
               +++  EL        A+LK+L A+
Sbjct: 242389 GKSMTMELKAWLRERMAILKQLTAS 242463


>gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 5, whole genome shotgun
              sequence
          Length = 1098047

 Score = 71.2 bits (173), Expect = 4e-13
 Identities = 39/106 (36%), Positives = 61/106 (57%), Gaps = 4/106 (3%)
 Frame = -3

Query: 20     IELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTT 79
              +++T  +F++ VL+S     + F APW GHCK+++P WD  A    D +  ++A VD  T
Sbjct: 692820 LQVTAASFEKDVLQSPGVVAVAFTAPWCGHCKRLEPVWDQAAREL-DGQGAVLAWVD-AT 692647

Query: 80     GGKPLCEKYGVRGYPTIKYF----NPPDEEGEDYKGGRSLDELKKF 121
                  L ++Y V GYPTIK F        E+ ED++G RS++ +  F
Sbjct: 692646 AETSLAQRYQVTGYPTIKIFAKAGTKSYEDAEDHQGDRSVEGVVDF 692509


>gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 6, whole genome shotgun
              sequence
          Length = 1035082

 Score = 47.0 bits (110), Expect = 8e-06
 Identities = 34/121 (28%), Positives = 53/121 (43%), Gaps = 1/121 (0%)
 Frame = -1

Query: 4      RSLTLLCAAAGASAGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLAST 63
              R L        A    I LT   FD  +   G    I+F APW  HC    P +  +A  
Sbjct: 299887 RRLVTAAKTDKADVSLITLTSRTFDGAI-SDGNVWLIEFYAPWCSHCTSFLPTYIEIAEE 299711

Query: 64     FEDS-KKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFA 122
              F  S K++ +A ++  +  K L  ++ V G+PT  ++         ++G R+   L KFA
Sbjct: 299710 FHKSDKRIKVAKIN-GSEEKALASRFDVYGFPT--FYVIVGWSVYSFEGQRTKQNLIKFA 299540

Query: 123    E 123
              +
Sbjct: 299539 D 299537


  Database: P.tricornutum/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 23,733,684
  Number of sequences in database:  31
  
Lambda     K      H
   0.311    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,609,389
Number of Sequences: 31
Number of extensions: 78251
Number of successful extensions: 361
Number of sequences better than 1.0e-05: 4
Number of HSP's better than  0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 265
Number of HSP's gapped (non-prelim): 158
length of query: 223
length of database: 7,911,228
effective HSP length: 94
effective length of query: 129
effective length of database: 7,908,314
effective search space: 1020172506
effective search space used: 1020172506
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 109 (46.6 bits)