TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= EhSep2_emiliania_huxley; gi|182639182|sp|Q50KB1.2|SEP2_EMIHU
RecName: Full=Protein disulfide-isomerase-like protein EhSep2; Flags:
Precursor # QUERY
         (223 letters)

Database: P.pallidum/genome.fa 
           42 sequences; 32,942,533 total letters

Searching..........................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GL290985.1| Polysphondylium pallidum PN500 unplaced genomic s...   115   3e-26
gb|GL290993.1| Polysphondylium pallidum PN500 unplaced genomic s...    72   2e-13
gb|GL290996.1| Polysphondylium pallidum PN500 unplaced genomic s...    65   3e-11
gb|GL290991.1| Polysphondylium pallidum PN500 unplaced genomic s...    57   8e-09
gb|GL291009.1| Polysphondylium pallidum PN500 unplaced genomic s...    53   2e-07

>gb|GL290985.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold3,
               whole genome shotgun sequence
          Length = 2290287

 Score =  115 bits (288), Expect = 3e-26
 Identities = 70/202 (34%), Positives = 107/202 (52%), Gaps = 5/202 (2%)
 Frame = +3

Query: 15      ASAGAIELTPDNFDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDSLASTFEDSKKVLIAD 74
               A +  I+LTP+NFD  VL   K   ++F APW GHCKK+ PD++ LA+T+   K V IA 
Sbjct: 1578066 APSNVIDLTPENFDAEVLNKDKDVLVEFYAPWCGHCKKLAPDYEILANTYAGDKHVGIAK 1578245

Query: 75      VDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELG----PGC 130
               VDC +  K LC KY ++G+PT+K+F   ++EGE Y+ GR L+    F     G     G 
Sbjct: 1578246 VDCDS-HKELCSKYDIKGFPTLKWFPKDNKEGEKYEQGRELETFITFINKNAGTHRVKGG 1578422

Query: 131     SVDLMDNCSEEQKGKLKEYIDMAPEKRTEMLETLKKELAD-AESTHEALLKELQATYKES 189
                +       E+      +++D   + RTE+L    K ++  AES     LK     Y + 
Sbjct: 1578423 RLLPSAGRVEQLDALAAKFVDATEKARTEILAEANKVVSSLAES-----LKPDGKIYVKI 1578587

Query: 190     MDKLEKLKEESAPKIKLLKAAT 211
               M+ ++K K+  + +I  +   T
Sbjct: 1578588 MEAIQKTKDYVSNEINRVTKLT 1578653



 Score =  113 bits (283), Expect = 1e-25
 Identities = 65/156 (41%), Positives = 87/156 (55%), Gaps = 1/156 (0%)
 Frame = +3

Query: 1       MALRSLTLLCAAAGASAG-AIELTPDNFDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDS 59
               + + +L  LC A  A+ G  + L+PDNFD +V    K  F+KF APW GHCKK+ PD++ 
Sbjct: 1577670 LVISALIALCVAFVAADGNVVVLSPDNFDTVV-DGTKTVFVKFYAPWCGHCKKLAPDYEV 1577846

Query: 60      LASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELK 119
               +A TF  SK+V+IA +DC    K LC KY V GYPT+K F    +E +DY G RS++E+ 
Sbjct: 1577847 IADTFAGSKQVVIAKLDCDV-HKELCGKYDVSGYPTLKVF-AKSKEAKDYNGMRSIEEIV 1578020

Query: 120     KFAENELGPGCSVDLMDNCSEEQKGKLKEYIDMAPE 155
                F  N  G    V          K      ID+ PE
Sbjct: 1578021 TFVNNAAGTNVRV----------KKAPSNVIDLTPE 1578098


>gb|GL290993.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold11,
               whole genome shotgun sequence
          Length = 1505626

 Score = 72.4 bits (176), Expect = 2e-13
 Identities = 35/98 (35%), Positives = 55/98 (56%), Gaps = 1/98 (1%)
 Frame = +1

Query: 27      FDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCE 86
               ++++VL   K   ++F APW GHCK ++P +  L     ++  V+IA VD T    P   
Sbjct: 1452340 YEQIVLDETKDVLVEFYAPWCGHCKSLEPIYKQLGDYMAENPHVVIAKVDATANDVP--P 1452513

Query: 87      KYGVRGYPTIKYFNPPDEEGE-DYKGGRSLDELKKFAE 123
               +  +RG+PTIKYF   D++   +Y G R L  L +F +
Sbjct: 1452514 ELAIRGFPTIKYFKATDKKNPVEYNGQRDLASLVEFIQ 1452627



 Score = 62.8 bits (151), Expect = 2e-10
 Identities = 30/80 (37%), Positives = 43/80 (53%)
 Frame = +3

Query: 20      IELTPDNFDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDSLASTFEDSKKVLIADVDCTT 79
               I +   NF+E++ +    A + F APW GHCK +KP W   + +   +KKV +  VDCT 
Sbjct: 1451007 INMNEVNFNEVITEHD-LALVMFFAPWCGHCKNLKPHWSEASKSLATNKKVALGKVDCTV 1451183

Query: 80      GGKPLCEKYGVRGYPTIKYF 99
                   LC+   V  YPT+  F
Sbjct: 1451184 EA-TLCQLNKVEYYPTLVLF 1451240



 Score = 56.6 bits (135), Expect = 1e-08
 Identities = 29/98 (29%), Positives = 50/98 (51%), Gaps = 1/98 (1%)
 Frame = +1

Query: 25     DNFDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPL 84
              + FD++VL   K   ++F APW  +   +KP ++ L         ++I  +D T    P 
Sbjct: 935677 NTFDQIVLDETKDVLVEFYAPWCPYIWSLKPTYEKLGDYMAKYPHIVIGKIDATANDVP- 935853

Query: 85     CEKYGVRGYPTIKYFNPPDEEGE-DYKGGRSLDELKKF 121
                +  +RG+PTIK+F   D++    ++G R L  L +F
Sbjct: 935854 -PELDIRGFPTIKFFKANDKKNPVTFEGERDLATLVEF 935964



 Score = 54.7 bits (130), Expect = 5e-08
 Identities = 30/81 (37%), Positives = 44/81 (54%)
 Frame = -1

Query: 17     AGAIELTPDNFDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDSLASTFEDSKKVLIADVD 76
              +  +EL   NF E+   +G+  FI F APW G CKK  P ++ ++S F  +  V    ++
Sbjct: 439603 SSVLELDSVNFAEV--NNGQKWFIVFYAPWCGFCKKYMPGFEKVSSQF--AGNVRFGKIN 439436

Query: 77     CTTGGKPLCEKYGVRGYPTIK 97
              C    K +CE Y + GYPT K
Sbjct: 439435 CDE-HKSICELYNIPGYPTFK 439376



 Score = 47.8 bits (112), Expect = 6e-06
 Identities = 30/110 (27%), Positives = 45/110 (40%), Gaps = 13/110 (11%)
 Frame = +2

Query: 3      LRSLTLLCAAA-------------GASAGAIELTPDNFDELVLKSGKAAFIKFLAPWXGH 49
              L ++ +LCA A              +    I +   NF+E V+       + F   W GH
Sbjct: 934310 LATIVILCAVAIGARHSHFEEEDGPSDDNVISMNETNFNE-VITGHYLVLVVFCMSWSGH 934486

Query: 50     CKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYF 99
              CK +KP +   + +F  + KV    VDC      LC+   V   PT+  F
Sbjct: 934487 CKNLKPHYSEASKSFATNNKVAFGKVDCIVENN-LCQSNKVELLPTLILF 934633


>gb|GL290996.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold14,
              whole genome shotgun sequence
          Length = 1054919

 Score = 65.5 bits (158), Expect = 3e-11
 Identities = 34/118 (28%), Positives = 59/118 (50%), Gaps = 5/118 (4%)
 Frame = -3

Query: 14     GASAGAIELTPDNFDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDSLASTFEDSKKVLIA 73
              G  +  + L   NF+ LV K      ++F APW GHCK +KP+++  A       K  + 
Sbjct: 609777 GKGSSVVNLNKKNFESLVAKDEHIWMVEFYAPWCGHCKSLKPEYEKAAKNLAGIAK--LG 609604

Query: 74     DVDCTTGGKPLCEKYGVRGYPTIKYFN-----PPDEEGEDYKGGRSLDELKKFAENEL 126
               ++C    K LC  + ++G+PT+K+F+        +  EDY+  RS   +  +  ++L
Sbjct: 609603 AINCDV-EKELCGAFEIKGFPTLKFFSHKLAAKGMKTPEDYQQERSASAIINYMTSKL 609433


>gb|GL290991.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold9,
               whole genome shotgun sequence
          Length = 2264991

 Score = 57.4 bits (137), Expect = 8e-09
 Identities = 31/97 (31%), Positives = 51/97 (52%)
 Frame = +1

Query: 22      LTPDNFDELVLKSGKAAFIKFLAPWXGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGG 81
               LT  NF + +    K   + F +P  GHC++MKP +   + T +       A  DC +  
Sbjct: 1819051 LTKRNFTQFIQTHPKT-LVMFYSPGCGHCERMKPAYAEASITVQKEHLGDFAAYDCNSE- 1819224

Query: 82      KPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDEL 118
                 +CEKY ++G+PT  YF+   E  + Y G R++D++
Sbjct: 1819225 MSICEKYKIKGFPTAIYFHNAKESAQ-YTGDRTVDDM 1819332


>gb|GL291009.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig12,
              whole genome shotgun sequence
          Length = 1458135

 Score = 53.1 bits (126), Expect = 2e-07
 Identities = 30/85 (35%), Positives = 46/85 (54%), Gaps = 1/85 (1%)
 Frame = +3

Query: 44     APWXGHCKKMKPDWDSLASTFEDSK-KVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPP 102
              APW G+CK++ P W  L      +K  V IA ++C    K +C +  VRGYPTIK F   
Sbjct: 778524 APWCGYCKRLAPVWSELGIYAHRNKLNVNIARINC-DDNKGICGENDVRGYPTIKLF--V 778694

Query: 103    DEEGEDYKGGRSLDELKKFAENELG 127
              +   ++Y+ GR+ +    + +  LG
Sbjct: 778695 NGTKKEYRSGRTKEAFIGYLDKMLG 778769


  Database: P.pallidum/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 32,942,533
  Number of sequences in database:  42
  
Lambda     K      H
   0.311    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,017,779
Number of Sequences: 42
Number of extensions: 68847
Number of successful extensions: 270
Number of sequences better than 1.0e-05: 5
Number of HSP's better than  0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 210
Number of HSP's gapped (non-prelim): 100
length of query: 223
length of database: 10,980,844
effective HSP length: 97
effective length of query: 126
effective length of database: 10,976,770
effective search space: 1383073020
effective search space used: 1383073020
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 110 (47.0 bits)