TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= EhSep2_emiliania_huxley; gi|182639182|sp|Q50KB1.2|SEP2_EMIHU
RecName: Full=Protein disulfide-isomerase-like protein EhSep2; Flags:
Precursor # QUERY
         (223 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   114   5e-26
gb|GG738904.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    75   5e-14
gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    73   2e-13

>gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome shotgun
              sequence
          Length = 1001995

 Score =  114 bits (286), Expect = 5e-26
 Identities = 69/196 (35%), Positives = 109/196 (55%), Gaps = 8/196 (4%)
 Frame = -2

Query: 21     ELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTG 80
              +L+P  FD++VL S K   +KF APW GHCKKM PD++ +A  F + K V++A VDC   
Sbjct: 689115 DLSPQTFDKIVLDSEKNVLVKFYAPWCGHCKKMAPDYEKVAKAFLNEKSVVVAHVDCDK- 688939

Query: 81     GKPLCEKYGVRGYPTIKYFNPPD-EEGEDYKGGRSLDELKKFAENELGPGCSVDLMDNCS 139
               + LC KYGV+GYPT+K+F   + +E E+Y  GR      +F  N+ G   ++   +   
Sbjct: 688938 YRDLCSKYGVQGYPTLKFFPAKENKEAEEYNSGREAPAFLEFLNNKAGTSRNI---EGAL 688768

Query: 140    EEQKGKLKEYIDMAPEKR---TEMLETLKKELADAESTHEALLKELQA---TYKESMDKL 193
               E+ G L   +   P K+      +E  KK +A+ E+T  +L+   +A    Y ++M ++
Sbjct: 688767 SEKAGVLASMV--GPVKKFLAASTVEDKKKVIAEVEATVSSLVGAAKANADVYAKAMARI 688594

Query: 194    -EKLKEESAPKIKLLK 208
               EK  E  A ++  L+
Sbjct: 688593 VEKGAEYVATEVARLE 688546



 Score = 74.7 bits (182), Expect = 6e-14
 Identities = 39/106 (36%), Positives = 57/106 (53%), Gaps = 2/106 (1%)
 Frame = -2

Query: 18     GAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKK--VLIADV 75
              G I+LT  N    VL   K   ++F APW GHCK + P+   L      +K   V +A +
Sbjct: 689487 GIIDLTNANAAS-VLDGSKGVLVEFYAPWCGHCKNLAPEMVKLGQALIKAKPTIVAVAKI 689311

Query: 76     DCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKF 121
              +C    + +C KYGV+GYPT+KYF     E  +Y  GR+++ +  F
Sbjct: 689310 NCDNE-RDVCSKYGVQGYPTLKYFPRGSSEPIEYNSGRTVEAMVDF 689176


>gb|GG738904.1| Naegleria gruberi genomic scaffold NAEGRscaffold_60, whole genome shotgun
              sequence
          Length = 297120

 Score = 75.1 bits (183), Expect = 5e-14
 Identities = 49/147 (33%), Positives = 73/147 (49%), Gaps = 7/147 (4%)
 Frame = +3

Query: 8      LLCAAAGASAGAIELTPDNFDELVLKSG-KAAFIKFLAPWUGHCKKMKPDWDSLASTFED 66
              L   A G+S   +EL   NF+  +  +  K  FIKF APW GHCK++   W  LA   ED
Sbjct: 194067 LFVQADGSSGVVLELNTQNFETTIQSNPQKVFFIKFFAPWCGHCKRLAGTWSELAKELED 194246

Query: 67     S-----KKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKF 121
              S     + V +A V+C      +  KY +RGYPT+  F    +   +Y G R++ + K F
Sbjct: 194247 SSVPHFQNVQLAKVNCDE-HPDIRRKYSIRGYPTLVLF-VGGKPVTEYYGYRTVPKFKSF 194420

Query: 122    AENELGPGCSVDLMDNCSE-EQKGKLK 147
               ++      S   +D  +E E + K+K
Sbjct: 194421 LDDVFSSAKSSGSVDQVTEVELESKIK 194501


>gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_19, whole genome shotgun
              sequence
          Length = 467654

 Score = 73.2 bits (178), Expect = 2e-13
 Identities = 52/179 (29%), Positives = 87/179 (48%), Gaps = 6/179 (3%)
 Frame = +3

Query: 10     CAAAGASAGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKK 69
              C    A    ++LT  NF+E  L+  + A I+F APW GHCKK+ P+++ LA  F  ++K
Sbjct: 427101 CTFVFAQDSVVDLTSKNFEEK-LQEKEFALIEFFAPWCGHCKKLVPEYNKLAEKFATNEK 427277

Query: 70     VLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELGPG 129
              V I  V+       +  K+ ++G+PTIK F    +   DY G R+ D +  +   + GP 
Sbjct: 427278 VNIFKVN-GDQESDVMSKFEIQGFPTIKLFK-NGKFFRDYDGERTADAIASWLHKKTGPV 427451

Query: 130    C----SVDLMDNCSEEQKGKLKEYIDMAPEKRTEMLETLKK--ELADAESTHEALLKEL 182
                   S + +D      K  +  ++       ++  ET KK  + AD +   E ++ E+
Sbjct: 427452 SIPIESAEALDQLKASSKVIVVGFVS------SKTSETYKKFLQAADDKDLEEFIVAEV 427610



 Score = 65.1 bits (157), Expect = 5e-11
 Identities = 32/100 (32%), Positives = 55/100 (55%), Gaps = 2/100 (2%)
 Frame = +3

Query: 26     NFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLC 85
              +FD+LV+ +     ++F APW GHCK ++P +  LA   +    ++IA +D +    P+ 
Sbjct: 428157 SFDDLVINNDNDVLLEFYAPWCGHCKSLEPKYTQLAEELKSVSGLVIAKIDASENDTPI- 428333

Query: 86     EKYGVRGYPTIKYFNPPDEEGED--YKGGRSLDELKKFAE 123
                  + G+PTI YF P   +     Y+G R+++ LK F +
Sbjct: 428334 ---NIEGFPTI-YFFPKGGKASPVLYEGDRTVESLKTFLQ 428441


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.311    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,051,455
Number of Sequences: 784
Number of extensions: 98872
Number of successful extensions: 495
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 78
Number of HSP's successfully gapped in prelim test: 51
Number of HSP's that attempted gapping in prelim test: 281
Number of HSP's gapped (non-prelim): 354
length of query: 223
length of database: 13,654,695
effective HSP length: 98
effective length of query: 125
effective length of database: 13,577,863
effective search space: 1697232875
effective search space used: 1697232875
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 111 (47.4 bits)