TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= EhSep2_emiliania_huxley; gi|182639182|sp|Q50KB1.2|SEP2_EMIHU
RecName: Full=Protein disulfide-isomerase-like protein EhSep2; Flags:
Precursor # QUERY
(223 letters)
Database: N.gruberi/genome.fa
784 sequences; 40,964,085 total letters
Searching....................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 114 5e-26
gb|GG738904.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 75 5e-14
gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 73 2e-13
>gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome shotgun
sequence
Length = 1001995
Score = 114 bits (286), Expect = 5e-26
Identities = 69/196 (35%), Positives = 109/196 (55%), Gaps = 8/196 (4%)
Frame = -2
Query: 21 ELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTG 80
+L+P FD++VL S K +KF APW GHCKKM PD++ +A F + K V++A VDC
Sbjct: 689115 DLSPQTFDKIVLDSEKNVLVKFYAPWCGHCKKMAPDYEKVAKAFLNEKSVVVAHVDCDK- 688939
Query: 81 GKPLCEKYGVRGYPTIKYFNPPD-EEGEDYKGGRSLDELKKFAENELGPGCSVDLMDNCS 139
+ LC KYGV+GYPT+K+F + +E E+Y GR +F N+ G ++ +
Sbjct: 688938 YRDLCSKYGVQGYPTLKFFPAKENKEAEEYNSGREAPAFLEFLNNKAGTSRNI---EGAL 688768
Query: 140 EEQKGKLKEYIDMAPEKR---TEMLETLKKELADAESTHEALLKELQA---TYKESMDKL 193
E+ G L + P K+ +E KK +A+ E+T +L+ +A Y ++M ++
Sbjct: 688767 SEKAGVLASMV--GPVKKFLAASTVEDKKKVIAEVEATVSSLVGAAKANADVYAKAMARI 688594
Query: 194 -EKLKEESAPKIKLLK 208
EK E A ++ L+
Sbjct: 688593 VEKGAEYVATEVARLE 688546
Score = 74.7 bits (182), Expect = 6e-14
Identities = 39/106 (36%), Positives = 57/106 (53%), Gaps = 2/106 (1%)
Frame = -2
Query: 18 GAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKK--VLIADV 75
G I+LT N VL K ++F APW GHCK + P+ L +K V +A +
Sbjct: 689487 GIIDLTNANAAS-VLDGSKGVLVEFYAPWCGHCKNLAPEMVKLGQALIKAKPTIVAVAKI 689311
Query: 76 DCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKF 121
+C + +C KYGV+GYPT+KYF E +Y GR+++ + F
Sbjct: 689310 NCDNE-RDVCSKYGVQGYPTLKYFPRGSSEPIEYNSGRTVEAMVDF 689176
>gb|GG738904.1| Naegleria gruberi genomic scaffold NAEGRscaffold_60, whole genome shotgun
sequence
Length = 297120
Score = 75.1 bits (183), Expect = 5e-14
Identities = 49/147 (33%), Positives = 73/147 (49%), Gaps = 7/147 (4%)
Frame = +3
Query: 8 LLCAAAGASAGAIELTPDNFDELVLKSG-KAAFIKFLAPWUGHCKKMKPDWDSLASTFED 66
L A G+S +EL NF+ + + K FIKF APW GHCK++ W LA ED
Sbjct: 194067 LFVQADGSSGVVLELNTQNFETTIQSNPQKVFFIKFFAPWCGHCKRLAGTWSELAKELED 194246
Query: 67 S-----KKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKF 121
S + V +A V+C + KY +RGYPT+ F + +Y G R++ + K F
Sbjct: 194247 SSVPHFQNVQLAKVNCDE-HPDIRRKYSIRGYPTLVLF-VGGKPVTEYYGYRTVPKFKSF 194420
Query: 122 AENELGPGCSVDLMDNCSE-EQKGKLK 147
++ S +D +E E + K+K
Sbjct: 194421 LDDVFSSAKSSGSVDQVTEVELESKIK 194501
>gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_19, whole genome shotgun
sequence
Length = 467654
Score = 73.2 bits (178), Expect = 2e-13
Identities = 52/179 (29%), Positives = 87/179 (48%), Gaps = 6/179 (3%)
Frame = +3
Query: 10 CAAAGASAGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKK 69
C A ++LT NF+E L+ + A I+F APW GHCKK+ P+++ LA F ++K
Sbjct: 427101 CTFVFAQDSVVDLTSKNFEEK-LQEKEFALIEFFAPWCGHCKKLVPEYNKLAEKFATNEK 427277
Query: 70 VLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELGPG 129
V I V+ + K+ ++G+PTIK F + DY G R+ D + + + GP
Sbjct: 427278 VNIFKVN-GDQESDVMSKFEIQGFPTIKLFK-NGKFFRDYDGERTADAIASWLHKKTGPV 427451
Query: 130 C----SVDLMDNCSEEQKGKLKEYIDMAPEKRTEMLETLKK--ELADAESTHEALLKEL 182
S + +D K + ++ ++ ET KK + AD + E ++ E+
Sbjct: 427452 SIPIESAEALDQLKASSKVIVVGFVS------SKTSETYKKFLQAADDKDLEEFIVAEV 427610
Score = 65.1 bits (157), Expect = 5e-11
Identities = 32/100 (32%), Positives = 55/100 (55%), Gaps = 2/100 (2%)
Frame = +3
Query: 26 NFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLC 85
+FD+LV+ + ++F APW GHCK ++P + LA + ++IA +D + P+
Sbjct: 428157 SFDDLVINNDNDVLLEFYAPWCGHCKSLEPKYTQLAEELKSVSGLVIAKIDASENDTPI- 428333
Query: 86 EKYGVRGYPTIKYFNPPDEEGED--YKGGRSLDELKKFAE 123
+ G+PTI YF P + Y+G R+++ LK F +
Sbjct: 428334 ---NIEGFPTI-YFFPKGGKASPVLYEGDRTVESLKTFLQ 428441
Database: N.gruberi/genome.fa
Posted date: Feb 9, 2011 9:02 AM
Number of letters in database: 40,964,085
Number of sequences in database: 784
Lambda K H
0.311 0.131 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,051,455
Number of Sequences: 784
Number of extensions: 98872
Number of successful extensions: 495
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 78
Number of HSP's successfully gapped in prelim test: 51
Number of HSP's that attempted gapping in prelim test: 281
Number of HSP's gapped (non-prelim): 354
length of query: 223
length of database: 13,654,695
effective HSP length: 98
effective length of query: 125
effective length of database: 13,577,863
effective search space: 1697232875
effective search space used: 1697232875
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 111 (47.4 bits)