TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= EhSep2_emiliania_huxley; gi|182639182|sp|Q50KB1.2|SEP2_EMIHU
RecName: Full=Protein disulfide-isomerase-like protein EhSep2; Flags:
Precursor # QUERY
         (223 letters)

Database: H.arabidopsidis/genome.fa 
           5422 sequences; 67,459,135 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABWE01000013.1| Hyaloperonospora parasitica strain Emoy2 v-7....    94   2e-19
gb|ABWE01000955.1| Hyaloperonospora parasitica strain Emoy2 v-7....    85   9e-17
gb|ABWE01000083.1| Hyaloperonospora parasitica strain Emoy2 v-7....    78   1e-14
gb|ABWE01000015.1| Hyaloperonospora parasitica strain Emoy2 v-7....    72   6e-13
gb|ABWE01000063.1| Hyaloperonospora parasitica strain Emoy2 v-7....    68   1e-11
gb|ABWE01000027.1| Hyaloperonospora parasitica strain Emoy2 v-7....    60   2e-09
gb|ABWE01000549.1| Hyaloperonospora parasitica strain Emoy2 v-7....    55   6e-08

>gb|ABWE01000013.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont43.6, whole genome
              shotgun sequence
          Length = 139795

 Score = 94.0 bits (232), Expect = 2e-19
 Identities = 47/118 (39%), Positives = 68/118 (57%)
 Frame = -3

Query: 15     ASAGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIAD 74
              A+     LTPDNFDE V+   K   +KF APW GHCK + P ++++A+ F+ +  V++AD
Sbjct: 131876 AAGDVTVLTPDNFDE-VIDGSKHVLVKFYAPWCGHCKSLAPTYETVATAFKKADDVVVAD 131700

Query: 75     VDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELGPGCSV 132
              +D     K L  K+GV G+PT+KYF     E   Y+GGRS ++   F   + G    V
Sbjct: 131699 LD-ADNYKDLGTKFGVTGFPTLKYFPKGSTEPTAYEGGRSEEDFVAFLNGKAGTNVQV 131529



 Score = 93.6 bits (231), Expect = 2e-19
 Identities = 47/112 (41%), Positives = 63/112 (56%)
 Frame = -3

Query: 22     LTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGG 81
              LT  +FDE V+ S K A ++F APW GHCK + P ++ +   FE    VLIA VD T   
Sbjct: 131501 LTDSDFDEQVIHSKKHALVEFYAPWCGHCKSLAPIYEEVGGIFEGEDDVLIAKVDATANP 131322

Query: 82     KPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELGPGCSVD 133
              + L ++Y V+GYPT+ YF P  +E EDY  GR      +F   + G   S D
Sbjct: 131321 E-LAKRYNVKGYPTLFYFPPGADEPEDYSNGRDKASFVEFINEQAGTHRSED 131169


>gb|ABWE01000955.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont107.4, whole
             genome shotgun sequence
          Length = 23006

 Score = 84.7 bits (208), Expect = 9e-17
 Identities = 44/111 (39%), Positives = 66/111 (59%), Gaps = 3/111 (2%)
 Frame = -2

Query: 17    AGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDS-KKVLIADV 75
             +  ++LT  +F+  VL S +   IKF APW GHCK++ P W+ L+ T ++S  +  +A V
Sbjct: 10435 SAVVQLTTASFERQVLGSNEPWLIKFYAPWCGHCKRLAPTWNKLSRTLQESGSQTHVAKV 10256

Query: 76    DCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGE--DYKGGRSLDELKKFAEN 124
             DCT   + +C ++GV GYP++ Y      EG+   YKGGRSL     F E+
Sbjct: 10255 DCTV-HRRVCSRFGVHGYPSLFYI----REGQVYRYKGGRSLPAFLDFVES 10118



 Score = 67.0 bits (162), Expect = 2e-11
 Identities = 42/120 (35%), Positives = 60/120 (50%), Gaps = 1/120 (0%)
 Frame = -2

Query: 6     LTLLCAAAGASAGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFE 65
             L L   +    +  + LT   FD     S    FIKF APW  HCK++ P  D+L++  E
Sbjct: 10879 LALSSVSFRVHSAPVSLTEATFDHET--STGVWFIKFYAPWCSHCKQLAPTIDALSTAKE 10706

Query: 66    -DSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAEN 124
                  V +A+VDCTT  + +CE++ V  YPT+K       +  DY G R +  +  FA N
Sbjct: 10705 LQDTDVHVANVDCTT-ERSVCERFSVGSYPTLKVV--AHGKSYDYNGRRDVSTMATFATN 10535


>gb|ABWE01000083.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont159.8, whole
             genome shotgun sequence
          Length = 88009

 Score = 77.8 bits (190), Expect = 1e-14
 Identities = 39/109 (35%), Positives = 60/109 (55%), Gaps = 3/109 (2%)
 Frame = +2

Query: 17    AGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVD 76
             +  I+LT   FD++VL SG    ++F APW GHCK + P+W+  AS  + S K  +  ++
Sbjct: 67784 SSVIQLTDATFDDMVLNSGDVWLVEFYAPWCGHCKALAPEWEQAASDLKGSVK--LGALE 67957

Query: 77    CTTGGKPLCEKYGVRGYPTIKYFNP---PDEEGEDYKGGRSLDELKKFA 122
              T   +   E YG+ G+P+IK F P      + +DY G R+   +  FA
Sbjct: 67958 ATVNEEKTTE-YGIHGFPSIKVFGPNAMGPADAQDYTGERTASAITDFA 68101



 Score = 48.5 bits (114), Expect = 7e-06
 Identities = 26/93 (27%), Positives = 45/93 (48%)
 Frame = +2

Query: 22    LTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGG 81
             L  ++F   V +      ++F APW GHCK++ P++   A     +  V +A +DC    
Sbjct: 67382 LDDEDFRVQVQQDSGVWLVEFYAPWCGHCKELAPEYKKAAKAL--AGVVNVAAIDCQE-N 67552

Query: 82    KPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRS 114
             +   +++ V GYPTI  F     +   + G R+
Sbjct: 67553 EGFVQEFAVSGYPTIMIFGEDKTKPTMFDGDRT 67651


>gb|ABWE01000015.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont40.12, whole
             genome shotgun sequence
          Length = 133533

 Score = 72.0 bits (175), Expect = 6e-13
 Identities = 54/186 (29%), Positives = 94/186 (50%), Gaps = 14/186 (7%)
 Frame = +1

Query: 1     MALRSLTLLCAAAGASAGAIELTPDNFDELVLKSGK---------AAFIKFLAPWUGHCK 51
             MA++ +      A A A       +  + LVLK            A  ++F APW GHCK
Sbjct: 88759 MAIKHVAHALVGALALAACATAFEEEDNVLVLKESDFADAVTGHDALLVEFYAPWCGHCK 88938

Query: 52    KMKPDWDSLASTFEDSK-KVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYK 110
             ++  D+ + A++ +     V +A VD T   K L E++ +RG+PT+K+F       +D+ 
Sbjct: 88939 QLAFDYAAAATSLKKKNLPVRLAKVDATVETK-LAEQFAIRGFPTLKFFKGDVNAVKDFD 89115

Query: 111   GGRSLDELKKFAENELGPGCSVDLMDNCSEEQKGKLKEYIDMAPEKRTEMLE----TLKK 166
             GGR+  E++K+   + GP  +V +++   E +  +LKE  D+      + +E     L +
Sbjct: 89116 GGRTAAEIEKWVVRKSGP--AVVIVETVQELE--ELKEANDVVVFAVVDAVEGEARALLE 89283

Query: 167   ELADAE 172
             +LADA+
Sbjct: 89284 KLADAD 89301



 Score = 64.3 bits (155), Expect = 1e-10
 Identities = 41/139 (29%), Positives = 65/139 (46%), Gaps = 16/139 (11%)
 Frame = +1

Query: 26    NFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLC 85
             +F E V+ + K   ++F APW GHCK + P ++ L   F D   ++IA +D         
Sbjct: 89902 DFQERVIDNEKDVLLEFYAPWCGHCKALAPKYEELGEAFADVDSIMIAKIDAAANEVDHA 90081

Query: 86    EKYGVRGYPTIKYFNPPDEEGE-DYKGGRSLDELKKFAE---------------NELGPG 129
                 V+G+PTI +F   D++    Y+G R +D   +F +               NE+G G
Sbjct: 90082 -GVEVQGFPTILFFPAMDKQNPIVYEGSRDVDGFTEFLKANAQKFELDGVQYGTNEIGKG 90258

Query: 130   CSVDLMDNCSEEQKGKLKE 148
                D ++  SEE   + KE
Sbjct: 90259 ---DEVEQTSEEDVKQKKE 90306


>gb|ABWE01000063.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont22.3, whole genome
             shotgun sequence
          Length = 95778

 Score = 67.8 bits (164), Expect = 1e-11
 Identities = 53/174 (30%), Positives = 82/174 (47%), Gaps = 13/174 (7%)
 Frame = +2

Query: 3     LRSLTLLCAAAGASAG-AIELTPDNFDELVLKSGKAA----FIKFLAPWUGHCKKMKPDW 57
             L SL LLC     SA   I L   +F+     +  A      ++F APW GHCKK+ P +
Sbjct: 81185 LWSLALLCTLQSVSASNVIVLGTADFEAKTQAATGATTGDWLVEFYAPWCGHCKKLVPIY 81364

Query: 58    DSLASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGG--RSL 115
             + +A   +    V   DV   T    L +++G+RG+PTI +F+     G+ YK G  R+L
Sbjct: 81365 EQVADELKGEVNVAKVDV---TEHPELGQRFGIRGFPTILHFS----HGKSYKFGSQRTL 81523

Query: 116   DELKKFAENELGP------GCSVDLMDNCSEEQKGKLKEYIDMAPEKRTEMLET 163
             DEL  FA              +  LMD  S++     ++++ +   K+  +L T
Sbjct: 81524 DELLLFARGGFEQVDGQVVAAAPGLMDFASQQLLQVKQDFVTLLATKKNVLLVT 81685


>gb|ABWE01000027.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont75.13, whole
             genome shotgun sequence
          Length = 119080

 Score = 60.1 bits (144), Expect = 2e-09
 Identities = 24/81 (29%), Positives = 44/81 (54%)
 Frame = -3

Query: 22    LTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGG 81
             L   N+D  +  S     + + APW  HC++  P+W+ +A  + ++ +V +  VDCT   
Sbjct: 35138 LDAQNYDTTMNDSDIVWLVDYYAPWCPHCRQFAPEWEKVAMFYSETSEVQVGAVDCTKNS 34959

Query: 82    KPLCEKYGVRGYPTIKYFNPP 102
             + +C +  +RGYP +K  + P
Sbjct: 34958 E-ICNQEDIRGYPGVKLHHVP 34899



 Score = 55.5 bits (132), Expect = 6e-08
 Identities = 26/85 (30%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
 Frame = -3

Query: 22    LTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGG 81
             L   NF  +V  +     + F +PW  HC+   P WD +++ ++D   ++   VDC+   
Sbjct: 59912 LDSSNFGAMVNDTQVIWLVDFYSPWCHHCQHFAPIWDEVSNLYKDVSSIVFGAVDCSKQN 59733

Query: 82    KPLCEKYGVRGYPTIK-YFNPPDEE 105
             + +C+  GV  YP +K Y   PD E
Sbjct: 59732 Q-ICDNEGVHSYPGVKMYHVSPDVE 59661


>gb|ABWE01000549.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont130.7, whole
            genome shotgun sequence
          Length = 36795

 Score = 55.5 bits (132), Expect = 6e-08
 Identities = 40/136 (29%), Positives = 65/136 (47%), Gaps = 5/136 (3%)
 Frame = -2

Query: 15   ASAGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFED---SKKVL 71
            + A   ELT + F+   LK      + F APW   CK+++P W  +A T       +++ 
Sbjct: 5741 SDATVTELTDETFEPF-LKEHHYVAVDFYAPWCVWCKRLEPVWTRVAKTLPSLHYGQQLR 5565

Query: 72   IADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEG-EDYKGGRSLDE-LKKFAENELGPG 129
            +A VDC    + LC K+ +R YP+I ++   D    E Y G R+++  + KF +   G  
Sbjct: 5564 VASVDCQAHPE-LCMKHYIRAYPSILFYKDGDTSPVEMYFGDRTVEAFVDKFKQLMAGEV 5388

Query: 130  CSVDLMDNCSEEQKGK 145
              V+ +     EQ  K
Sbjct: 5387 DVVEALKKKFHEQDKK 5340


  Database: H.arabidopsidis/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 67,459,135
  Number of sequences in database:  5422
  
Lambda     K      H
   0.311    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,079,308
Number of Sequences: 5422
Number of extensions: 197830
Number of successful extensions: 634
Number of sequences better than 1.0e-05: 7
Number of HSP's better than  0.0 without gapping: 334
Number of HSP's successfully gapped in prelim test: 35
Number of HSP's that attempted gapping in prelim test: 259
Number of HSP's gapped (non-prelim): 422
length of query: 223
length of database: 22,486,378
effective HSP length: 101
effective length of query: 122
effective length of database: 21,938,756
effective search space: 2676528232
effective search space used: 2676528232
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 113 (48.1 bits)