TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= EhSep2_emiliania_huxley; gi|182639182|sp|Q50KB1.2|SEP2_EMIHU
RecName: Full=Protein disulfide-isomerase-like protein EhSep2; Flags:
Precursor # QUERY
         (223 letters)

Database: C.muris/genome.fa 
           84 sequences; 9,245,251 total letters

Searching...................................................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DS989730.1| Cryptosporidium muris RN66 scf_1106632373475 geno...    75   9e-15
gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 geno...    62   1e-10
gb|DS989728.1| Cryptosporidium muris RN66 scf_1106632373407 geno...    61   2e-10
gb|DS989733.1| Cryptosporidium muris RN66 scf_1106632373425 geno...    52   1e-07

>gb|DS989730.1| Cryptosporidium muris RN66 scf_1106632373475 genomic scaffold, whole
              genome shotgun sequence
          Length = 723783

 Score = 75.5 bits (184), Expect = 9e-15
 Identities = 54/163 (33%), Positives = 81/163 (49%), Gaps = 16/163 (9%)
 Frame = +3

Query: 20     IELTPDNFDELVLKSGKAA-FIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCT 78
              +ELT  NFD++VL+  +++ FIKF APW GHC+ + PDW+ L   +    KV I  VD T
Sbjct: 460554 VELTDSNFDKIVLEDQESSWFIKFYAPWCGHCRNLAPDWEELG--YLAKGKVKIGKVDAT 460727

Query: 79     TGGKPLCEKYGVRGYPTIKYF---NPPDEEGEDYKGGRSLDELKKFAENELGPGCSVDLM 135
                   + ++Y ++G+PT+  F   N  +     Y G RS  +L +FA         V+ +
Sbjct: 460728 Q-HTMVAQRYQIQGFPTLLMFPSGNKKELNPIPYNGPRSASDLMEFAAKFQSRIIKVEQL 460904

Query: 136    -------DNCSEEQKGK-----LKEYIDMAPEKRTEMLETLKK 166
                     +NCS   KG      L   +D +   R + LE  KK
Sbjct: 460905 LNQEKFEENCS---KGLCVIAFLPNIVDSSVSDRQKYLEDYKK 461024



 Score = 53.9 bits (128), Expect = 3e-08
 Identities = 34/137 (24%), Positives = 61/137 (44%), Gaps = 8/137 (5%)
 Frame = +3

Query: 20     IELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTT 79
              +++   N    ++K       +F A W GHCK   P+++  AS  +    ++  + +   
Sbjct: 460137 VKVVTGNHLNKIIKEHPVVIAEFFAEWCGHCKAFAPEYEKAASALKGIVPLVAINNESDM 460316

Query: 80     GGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGR--------SLDELKKFAENELGPGCS 131
                     +YG++G+PT+K  +    + +DY G R        +L  LK  A + L    +
Sbjct: 460317 ------TEYGIKGFPTVKVLSSSFNKPKDYSGPRTSEGVVNAALAALKDVANSRLSGKKT 460478

Query: 132    VDLMDNCSEEQKGKLKE 148
               +   N SE  KGK K+
Sbjct: 460479 DNKKSNKSEFSKGKDKK 460529


>gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 genomic scaffold, whole genome
               shotgun sequence
          Length = 1306717

 Score = 62.0 bits (149), Expect = 1e-10
 Identities = 37/120 (30%), Positives = 63/120 (52%), Gaps = 1/120 (0%)
 Frame = -1

Query: 22      LTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDS-KKVLIADVDCTTG 80
               L    F++ V ++ +   + F APW GHC  ++P++ +  +    S  KV    VD T  
Sbjct: 1288120 LNSSTFEKFVSEN-EFVLVTFFAPWCGHCTALEPEFKATCAEMATSIPKVRCGSVDATE- 1287947

Query: 81      GKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELGPGCSVDLMDNCSE 140
                  L +++GV GYPTIK FN   E  +++ G RS +   ++     GP  ++ ++D+ SE
Sbjct: 1287946 NMELAQQFGVSGYPTIKLFN-GTENIQNFSGARSKENFLRWISKLTGP--AIQIVDSMSE 1287776



 Score = 60.1 bits (144), Expect = 4e-10
 Identities = 28/95 (29%), Positives = 50/95 (52%)
 Frame = -1

Query: 27      FDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCE 86
               F+E+V +  K   I+  A W GHCK ++P ++ LA   +D++ ++IA ++      P  E
Sbjct: 1287121 FEEIVFQKDKDVLIEIYAQWCGHCKNLEPIYNQLAEEMKDNENIVIAKINGPANDIPF-E 1286945

Query: 87      KYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKF 121
                +  R +PTI +          Y G R+++  K+F
Sbjct: 1286944 GFSPRAFPTILFVRAGTRTAIPYDGKRTVEAFKEF 1286840



 Score = 53.9 bits (128), Expect = 3e-08
 Identities = 35/132 (26%), Positives = 65/132 (49%), Gaps = 3/132 (2%)
 Frame = -1

Query: 21      ELTPDNFDELVLKSGKAA--FIKFLAPWUGHCKKMKPDWDSLASTF-EDSKKVLIADVDC 77
               +L   +F+E VL+  +    FI F APW G+C+++ P+    A  F  ++  + I  VDC
Sbjct: 1124794 DLQQKDFEEKVLRKNENITWFIYFFAPWCGYCQRIYPEVVKTAEYFILNNNNIQIGKVDC 1124615

Query: 78      TTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELGPGCSVDLMDN 137
               T   + +C+K  ++ YPT K F       +  K  R++ +  +F E+   P  +      
Sbjct: 1124614 TIQTE-ICKKEKIQAYPTFKLFLNESYVKKYEKPKRTVKDFIRFIESSKNPQYTHITSQQ 1124438

Query: 138     CSEEQKGKLKEY 149
                 +E K +++ +
Sbjct: 1124437 QLDEIKAEIRNF 1124402


>gb|DS989728.1| Cryptosporidium muris RN66 scf_1106632373407 genomic scaffold, whole
              genome shotgun sequence
          Length = 965821

 Score = 61.2 bits (147), Expect = 2e-10
 Identities = 41/140 (29%), Positives = 69/140 (49%), Gaps = 11/140 (7%)
 Frame = +1

Query: 20     IELTPDNFDELVLKS--GKAAFIKFLAPWUGHCKKMKPDWDSLAS------TFEDSKKVL 71
              +E+  + FD+ V  +   +A FI F APW GHCK +KP W  LA       T +D  +V 
Sbjct: 274786 LEIVANTFDKYVKSALDHEALFIMFYAPWCGHCKSLKPIWLKLAQKVHKMFTDKDKSRVT 274965

Query: 72     IADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEE---GEDYKGGRSLDELKKFAENELGP 128
              IA  D T     L     V+G PT+ +F    ++      Y+G R ++ L ++ +++   
Sbjct: 274966 IALYDITK--NDLLHGIDVQGIPTLFFFPFGSDKIMNKSKYEGSREIEGLMEYVQDQY-- 275133

Query: 129    GCSVDLMDNCSEEQKGKLKE 148
                  D +DN   ++K  +++
Sbjct: 275134 ---EDYLDNIRSKEKSHVRD 275184



 Score = 51.2 bits (121), Expect = 2e-07
 Identities = 29/82 (35%), Positives = 45/82 (54%), Gaps = 1/82 (1%)
 Frame = +1

Query: 20     IELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDS-KKVLIADVDCT 78
              I L    FD+ V  +     I F APW GHCK + P  D + +  E+S  K  IA +D T
Sbjct: 273604 IHLKSSTFDDFVTNNPNVLVI-FYAPWCGHCKGLVPILDPVVAEVEESGGKGKIAVIDAT 273780

Query: 79     TGGKPLCEKYGVRGYPTIKYFN 100
                 + L +++ ++GYPT+ +F+
Sbjct: 273781 VEVE-LAQRFDIKGYPTLLFFS 273843


>gb|DS989733.1| Cryptosporidium muris RN66 scf_1106632373425 genomic scaffold, whole
             genome shotgun sequence
          Length = 528544

 Score = 52.0 bits (123), Expect = 1e-07
 Identities = 28/92 (30%), Positives = 50/92 (54%), Gaps = 2/92 (2%)
 Frame = +1

Query: 29    ELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCEKY 88
             E++    +  FI F APW GHC+K++PD++ L     + K + IA +D   G +   E  
Sbjct: 69190 EVLNNVNQDTFIVFYAPWCGHCRKLEPDFNVLGQRLHNLKTLKIAKID---GSQNEIENI 69360

Query: 89    GVRGYPTIKYFNPPDEEGED--YKGGRSLDEL 118
              + GYP++  + P +++ +   Y G RS+  +
Sbjct: 69361 HILGYPSLMLY-PANKKSDPIWYDGDRSVHNM 69453


  Database: C.muris/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 9,245,251
  Number of sequences in database:  84
  
Lambda     K      H
   0.311    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,630,800
Number of Sequences: 84
Number of extensions: 18418
Number of successful extensions: 77
Number of sequences better than 1.0e-05: 4
Number of HSP's better than  0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 46
Number of HSP's gapped (non-prelim): 50
length of query: 223
length of database: 3,081,750
effective HSP length: 88
effective length of query: 135
effective length of database: 3,074,358
effective search space: 415038330
effective search space used: 415038330
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 106 (45.4 bits)