TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= EhSep2_emiliania_huxley; gi|182639182|sp|Q50KB1.2|SEP2_EMIHU
RecName: Full=Protein disulfide-isomerase-like protein EhSep2; Flags:
Precursor # QUERY
         (223 letters)

Database: B.hominis/genome.fa 
           54 sequences; 18,817,245 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-t...   106   7e-24
emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-t...    70   1e-18
emb|FN668649.1| Blastocystis hominis, Singapore isolate B (sub-t...    78   3e-15
emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-t...    61   3e-10

>emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun
               sequence assembly, scaffold_3
          Length = 1354787

 Score =  106 bits (265), Expect = 7e-24
 Identities = 70/210 (33%), Positives = 101/210 (48%), Gaps = 5/210 (2%)
 Frame = -1

Query: 14      GASAGAIELTPDNFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIA 73
               GA+     LTP  FD+ + + GK  F+KF APW GHCKK+ PD+  LA  F + K V+IA
Sbjct: 1190408 GATNNVKVLTPGTFDQTIGEEGKTVFVKFYAPWCGHCKKLAPDYKKLADVFAEEKNVIIA 1190229

Query: 74      DVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEEGEDYKGGRSLDELKKFAENELGPGCSVD 133
               +VD     K L   Y V GYPT+K F   + E  DYK  R L  L  F     G      
Sbjct: 1190228 EVDADK-YKDLARAYDVAGYPTLKLFK--NGEIVDYKEARDLASLVAFVNKHAGTARET- 1190061

Query: 134     LMDNCSEEQKGKLKEYIDMAPEKRTEMLETLKKELADAESTHEALLKELQATYKESMDKL 193
                 D   ++  G++ E +D   +K     E L+KE+       EA + E +  Y   + K+
Sbjct: 1190060 --DGSLKKSYGRIAE-VDEVLQKAEGFSEELQKEVEAILEKTEAKVAESKRIYASILKKI 1189890

Query: 194     EK-----LKEESAPKIKLLKAATPAPKAEG 218
                +     +++E A   K ++    AP  +G
Sbjct: 1189889 AEKGEKYVQDERARLTKFVEGENIAPLKKG 1189800



 Score = 54.3 bits (129), Expect = 4e-08
 Identities = 37/136 (27%), Positives = 66/136 (48%), Gaps = 12/136 (8%)
 Frame = -3

Query: 31      VLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFED----------SKKVLIADVDCTTG 80
               +L S    F  F   W GHC+ + P+++ LA +F             K V+IA+V+    
Sbjct: 1190778 ILCSCLNCFAPFNV*WCGHCQHLAPEYEILAESFARV*YLVRCVTFRKNVVIAEVNADE- 1190602

Query: 81      GKPLCEKYGVRGYPTIKYF--NPPDEEGEDYKGGRSLDELKKFAENELGPGCSVDLMDNC 138
                + L  ++ + GYPT+K+F    PD   E Y+G RS + L  +   ++G   S  L+   
Sbjct: 1190601 DRELANRFEIHGYPTLKFFPAGHPDAP-EVYQGERSAEALTNWLNEKMGRPRSHSLIPRY 1190425

Query: 139     SEEQKGKLKEYIDMAP 154
               + + +G+ ++   + P
Sbjct: 1190424 ARDGEGRYQQREGLDP 1190377


>emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome
              shotgun sequence assembly, scaffold_23
          Length = 240939

 Score = 70.5 bits (171), Expect(2) = 1e-18
 Identities = 52/165 (31%), Positives = 79/165 (47%), Gaps = 7/165 (4%)
 Frame = +3

Query: 42     FLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNP 101
              FL+ W GHC+ + P W+ L + F +   V IA++D     + L  ++ V GYPT+K F  
Sbjct: 143622 FLS*WCGHCRHLAPTWEELGAVFAEEPSVSIAELDADM-YRELASEFDVHGYPTLKLFQ- 143795

Query: 102    PDEEGEDYKGGRSLDELKKFAENELG----PGCSVDLMDN---CSEEQKGKLKEYIDMAP 154
                     Y+G RSL+ L +F     G    P  SVD         +E    LK   D   
Sbjct: 143796 GQAAPVPYEGDRSLESLVEFVNEHAGTARLPDGSVDAKFGRIPAVDEVIAGLKGVGDANL 143975

Query: 155    EKRTEMLETLKKELADAESTHEALLKELQATYKESMDKLEKLKEE 199
              EK  E +ET K E+  ++  + +++K+L    KE  +   K +EE
Sbjct: 143976 EKVKEAVETWKGEMEGSKKVYLSVVKKL----KEKGEGFLKSEEE 144098



 Score = 42.7 bits (99), Expect(2) = 7e-11
 Identities = 23/61 (37%), Positives = 32/61 (52%), Gaps = 10/61 (16%)
 Frame = +2

Query: 14     GASAGAIELTPDNFDELVLKSGKAAFIKFLAP----------WUGHCKKMKPDWDSLAST 63
              G +A  + LT +NFDE+V    K  FIKF AP          W GHC+ + P++   A +
Sbjct: 143057 G*AAAVVPLTKENFDEVV-NGEKNVFIKFYAPVLSSRLDNT*WCGHCQALAPEYTLFAES 143233

Query: 64     F 64
              F
Sbjct: 143234 F 143236



 Score = 40.4 bits (93), Expect(2) = 7e-11
 Identities = 19/63 (30%), Positives = 38/63 (60%), Gaps = 1/63 (1%)
 Frame = +3

Query: 70     VLIADVDCTTGGKPLCEKYGVRGYPTIKYFNP-PDEEGEDYKGGRSLDELKKFAENELGP 128
              +++A+VD  +  + L +++ + G+PT+K+F     ++ EDY G R+ + L  +  + LG 
Sbjct: 143286 LIVAEVDADSE-RELGDRFNIEGFPTLKFFPAGASDKPEDYDGDRTAEALVSWVNDRLGT 143462

Query: 129    GCS 131
               CS
Sbjct: 143463 FCS 143471



 Score = 39.3 bits (90), Expect(2) = 1e-18
 Identities = 17/24 (70%), Positives = 18/24 (75%)
 Frame = +1

Query: 22     LTPDNFDELVLKSGKAAFIKFLAP 45
              LTP NFDELV  SGK  F+KF AP
Sbjct: 143533 LTPSNFDELVKNSGKTVFVKFYAP 143604


>emb|FN668649.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome
              shotgun sequence assembly, scaffold_19
          Length = 315857

 Score = 77.8 bits (190), Expect = 3e-15
 Identities = 37/99 (37%), Positives = 58/99 (58%), Gaps = 1/99 (1%)
 Frame = +1

Query: 26     NFDELVLKSGKAAFIKFLAPWUGHCKKMKPDWDSLASTFEDSKKVLIADVDCTTGGKPLC 85
              +F+E+VL   K  F+KF APW GHCK + P ++ LA  ++D   V+IA++D T       
Sbjct: 224812 SFEEVVLDPKKNVFVKFYAPWCGHCKALAPTYEKLAEAYKDDADVVIAEMDATANE---V 224982

Query: 86     EKYGVRGYPTIKYFNPPDEEGE-DYKGGRSLDELKKFAE 123
                  +RG+PT+K++   +     DY+G R+L+ L  F E
Sbjct: 224983 AGLNIRGFPTLKFYKAGEPTAPVDYEGERTLEALTDFVE 225099



 Score = 75.1 bits (183), Expect = 2e-14
 Identities = 45/145 (31%), Positives = 74/145 (51%), Gaps = 17/145 (11%)
 Frame = +1

Query: 1      MALRSLTLLCAAAGA----SAGAIELTPDNFDELVLKSGKAAFIKFLAP----------- 45
              M L +  L+CA   A    + G + L  DNFD+ + +  ++  +KF AP           
Sbjct: 223687 MKLTAFLLICALVFAEFTENDGVLVLNDDNFDQAIAEH-ESLLVKFYAPVLFFVCVCVTL 223863

Query: 46     -WUGHCKKMKPDWDSLASTFED-SKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPD 103
               W GHCKK+ PD+ + A    +    + +A+VD T   K L +++ +RGYPT+K+F   +
Sbjct: 223864 *WCGHCKKLAPDYSAAARELRELDPPLYLAEVDATAAPK-LSQRFAIRGYPTLKFFK--N 224034

Query: 104    EEGEDYKGGRSLDELKKFAENELGP 128
                  DY  GRS  ++  + + + GP
Sbjct: 224035 GNAVDYDSGRSKADIVNYMKRKAGP 224109


>emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun
               sequence assembly, scaffold_2
          Length = 1458350

 Score = 61.2 bits (147), Expect = 3e-10
 Identities = 43/145 (29%), Positives = 69/145 (47%), Gaps = 15/145 (10%)
 Frame = -2

Query: 1       MALRSLTLLCAAAGASA----GAIELTPDNFDELVLKSGKAAFIKFLAP----------W 46
               M L ++ LL   A +S     G + L   NFD+ VL   K   + F AP          W
Sbjct: 1255774 MKLLTVVLLFVVALSSVQSQDGVLILNDKNFDQ-VLTEHKMVLVMFYAPVILEQLCNA*W 1255598

Query: 47      UGHCKKMKPDWDSLASTF-EDSKKVLIADVDCTTGGKPLCEKYGVRGYPTIKYFNPPDEE 105
                G+CKK+  +   +A      +  + IA +D T   +    KY +RG+PT+ +F    E 
Sbjct: 1255597 CGYCKKLDAELPGIAKQMMSHNPPIYIAKLDATANRESTL-KYHIRGFPTLLFFREGQE- 1255424

Query: 106     GEDYKGGRSLDELKKFAENELGPGC 130
                 +Y+GGR+ +++  F + + GP C
Sbjct: 1255423 -TEYEGGRTKEDIVSFLQKKSGPIC 1255352


  Database: B.hominis/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 18,817,245
  Number of sequences in database:  54
  
Lambda     K      H
   0.311    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,087,803
Number of Sequences: 54
Number of extensions: 54748
Number of successful extensions: 356
Number of sequences better than 1.0e-05: 5
Number of HSP's better than  0.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 195
Number of HSP's gapped (non-prelim): 254
length of query: 223
length of database: 6,272,415
effective HSP length: 93
effective length of query: 130
effective length of database: 6,267,393
effective search space: 814761090
effective search space used: 814761090
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 109 (46.6 bits)