TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: S.parasitica/genome.fa 
           1445 sequences; 53,132,636 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...   111   1e-40

>gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.24, whole
              genome shotgun sequence
          Length = 471063

 Score =  111 bits (278), Expect(3) = 1e-40
 Identities = 71/192 (36%), Positives = 91/192 (47%), Gaps = 35/192 (18%)
 Frame = +3

Query: 151    GRIACANVLSDLYAMGITECDNXXXXXXXXXXXXXXXREKVTPLMVKGFRDXXXXXXXXX 210
              GRIACANVLSDLYAMG+TE D                R+ VT  ++ GF D         
Sbjct: 142827 GRIACANVLSDLYAMGVTEVDTMLMILGVSRTMTDLERDVVTTQLIHGFNDLAREAGTNV 143006

Query: 211    XXXXXXXNPWIIIGGVA-----------------TVVCQPNEFIMPDSAVVGDVLVLTKP 253
                     NPW I+GGVA                 T +   +E I P++A  G +LVLTKP
Sbjct: 143007 TGGQTVMNPWPIVGGVAMSVRAGSPMFSSTKRNLTNIHLESEIIRPENATAGQMLVLTKP 143186

Query: 254    LG------------------TQVAVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNM 295
              LG                  TQ+AVNA+Q  D P RW+KV+  ++RE  + A+  A  +M
Sbjct: 143187 LGCALVLGGEFIASCCHAHSTQLAVNAYQMKDQPARWSKVESFLTREMADRAFSVASESM 143366

Query: 296    ATLNRTAAGLMH 307
              + LN   A LMH
Sbjct: 143367 SRLNVNGAKLMH 143402



 Score = 69.3 bits (168), Expect(2) = 4e-13
 Identities = 47/148 (31%), Positives = 77/148 (52%), Gaps = 24/148 (16%)
 Frame = +2

Query: 260    VNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNA----HAAT 315
              V   ++LD  +   +V+  +  +E     +        +   ++G MHT +A     +AT
Sbjct: 143297 VQGRKFLDAGDGRPRVQRRIRVDEPTQRQRRQAHAQGIITTCSSGNMHTDHA*HGVTSAT 143476

Query: 316    DITGFGILGHSQ-------------------NLAKQQRNEVSFVIHNLPIIAKMAAVSKA 356
              D+TGFGILGH+Q                   N+A  Q+ +V F +H LP+I  M AV++A
Sbjct: 143477 DVTGFGILGHAQYGNTIGV*LLLTLAFAAARNMATSQKADVDFELHTLPVIKDMLAVNEA 143656

Query: 357    -SGRFGLLQGTSAETSGGLLICLPREQA 383
               +G++ L  G S+ETSGGLL+ +  ++A
Sbjct: 143657 FNGQWRLELGFSSETSGGLLVSIDADRA 143740



 Score = 48.5 bits (114), Expect(3) = 1e-40
 Identities = 23/47 (48%), Positives = 31/47 (65%)
 Frame = +1

Query: 37     PFEPQALGLSPSWRLTGFSGMKGUGCKVPQEALLKLLAGLTRPDVRP 83
              PF P   GL   +RLT FS +KG GCK+PQE LL  ++G+   D++P
Sbjct: 142480 PFNPTDHGLREDFRLTDFSALKG*GCKLPQEKLLSYISGIAN-DLKP 142617



 Score = 46.6 bits (109), Expect(3) = 1e-40
 Identities = 20/35 (57%), Positives = 27/35 (77%), Gaps = 3/35 (8%)
 Frame = +2

Query: 118    GMDSCVIPLRH---GGLSLVQTTDFFYPLVEDPYM 149
              GMDS V+ ++H   G L ++ TTDFFYPLV+DPY+
Sbjct: 142676 GMDSSVVKIKHPKFGDLYMISTTDFFYPLVQDPYV 142780



 Score = 30.0 bits (66), Expect = 6.1
 Identities = 16/44 (36%), Positives = 22/44 (50%)
 Frame = +2

Query: 73     LAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALG 116
              L+ L  P +R P GRG++   E  S+ A    GA    T+P  G
Sbjct: 270878 LSSLVPPQMRSPFGRGIMYAPESISRIAARTHGACTRTTWPLHG 271009



 Score = 24.3 bits (51), Expect(2) = 4e-13
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +1

Query: 401    AWIVGIVEKGNRTARIIDKPRVIEV 425
              AWIVG V +G + AR+      +E+
Sbjct: 143827 AWIVGRVVQGTKQARLAPDVTFVEI 143901


  Database: S.parasitica/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 53,132,636
  Number of sequences in database:  1445
  
Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,661,076
Number of Sequences: 1445
Number of extensions: 390193
Number of successful extensions: 2059
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 66
Number of HSP's that attempted gapping in prelim test: 1550
Number of HSP's gapped (non-prelim): 1010
length of query: 448
length of database: 17,710,878
effective HSP length: 107
effective length of query: 341
effective length of database: 17,556,263
effective search space: 5986685683
effective search space used: 5986685683
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 64 (29.3 bits)