TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000032_1.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Homo sapiens # Complete
         (392 letters)

Database: S.parasitica/genome.fa 
           1445 sequences; 53,132,636 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...   163   4e-67
gb|GG743883.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    33   0.78 
gb|GG743967.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    30   5.1  

>gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.24, whole
              genome shotgun sequence
          Length = 471063

 Score =  163 bits (413), Expect(5) = 4e-67
 Identities = 93/192 (48%), Positives = 115/192 (59%), Gaps = 35/192 (18%)
 Frame = +3

Query: 100    GRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSV 159
              GRIACANVLSDLYAMGVTE D MLM+LGVS  MTD ERD V   +I GF D A EAGT+V
Sbjct: 142827 GRIACANVLSDLYAMGVTEVDTMLMILGVSRTMTDLERDVVTTQLIHGFNDLAREAGTNV 143006

Query: 160    TGGQTVLNPWIVLGGVA-----------------TTVCQPNEFIMPDNAVPGDVLVLTKP 202
              TGGQTV+NPW ++GGVA                 T +   +E I P+NA  G +LVLTKP
Sbjct: 143007 TGGQTVMNPWPIVGGVAMSVRAGSPMFSSTKRNLTNIHLESEIIRPENATAGQMLVLTKP 143186

Query: 203    LG------------------TQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNM 244
              LG                  TQ+AV  +Q  D P +W+K++  +T+E  + A+  A  +M
Sbjct: 143187 LGCALVLGGEFIASCCHAHSTQLAVNAYQMKDQPARWSKVESFLTREMADRAFSVASESM 143366

Query: 245    ARLNRTAAGLMH 256
              +RLN   A LMH
Sbjct: 143367 SRLNVNGAKLMH 143402



 Score = 66.6 bits (161), Expect(5) = 4e-67
 Identities = 41/106 (38%), Positives = 58/106 (54%), Gaps = 23/106 (21%)
 Frame = +2

Query: 251    AAGLMHTFNA----HAATDITGFGILGHAQ-------------------NLAKQQRNEVS 287
              ++G MHT +A     +ATD+TGFGILGHAQ                   N+A  Q+ +V 
Sbjct: 143423 SSGNMHTDHA*HGVTSATDVTGFGILGHAQYGNTIGV*LLLTLAFAAARNMATSQKADVD 143602

Query: 288    FVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQA 333
              F +H LPV+  M AV++A    + L  G   ETSGGLL+ +  ++A
Sbjct: 143603 FELHTLPVIKDMLAVNEAFNGQWRLELGFSSETSGGLLVSIDADRA 143740



 Score = 42.0 bits (97), Expect(5) = 4e-67
 Identities = 19/42 (45%), Positives = 26/42 (61%)
 Frame = +1

Query: 4      RESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESL 45
              R  FNP  + L + FRLT F+ LKG GCK+PQ+ L   +  +
Sbjct: 142474 RAPFNPTDHGLREDFRLTDFSALKG*GCKLPQEKLLSYISGI 142599



 Score = 40.4 bits (93), Expect(5) = 4e-67
 Identities = 16/35 (45%), Positives = 25/35 (71%), Gaps = 3/35 (8%)
 Frame = +2

Query: 67     GMDTCVIPLRH---GGLSLVQTTDYIYPIVDDPYM 98
              GMD+ V+ ++H   G L ++ TTD+ YP+V DPY+
Sbjct: 142676 GMDSSVVKIKHPKFGDLYMISTTDFFYPLVQDPYV 142780



 Score = 24.3 bits (51), Expect(5) = 4e-67
 Identities = 9/25 (36%), Positives = 15/25 (60%)
 Frame = +1

Query: 351    AWIIGIVEKGNRTARIIDKPRIIEV 375
              AWI+G V +G + AR+      +E+
Sbjct: 143827 AWIVGRVVQGTKQARLAPDVTFVEI 143901


>gb|GG743883.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.1, whole
              genome shotgun sequence
          Length = 1615555

 Score = 32.7 bits (73), Expect = 0.78
 Identities = 27/101 (26%), Positives = 47/101 (46%), Gaps = 2/101 (1%)
 Frame = +3

Query: 287    SFVIHNLPVLAKMAAVSKA--CGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPK 344
              ++V  NL  L KM    K+     + G+  G+C   SGGLL  L   Q++        P 
Sbjct: 126300 TYVTTNLR-LTKMTQAGKSLLAAKVQGVHAGSCTNLSGGLLAGLEEVQSS------SRPD 126458

Query: 345    YGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVN 385
              +GE +    + ++  G   A I  +  ++E+   V ++NV+
Sbjct: 126459 HGEPNPVQSVLLLTDGLANAGISSRSGLVELLEGVLSKNVS 126581


>gb|GG743967.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.85, whole
              genome shotgun sequence
          Length = 148989

 Score = 30.0 bits (66), Expect = 5.1
 Identities = 14/68 (20%), Positives = 36/68 (52%), Gaps = 8/68 (11%)
 Frame = -1

Query: 323    GLLICLPREQAARFCAEIKSPKYGEG----HQAWI----IGIVEKGNRTARIIDKPRIIE 374
              G ++ +P  +++R C  + +P+  +     H  ++      ++   NR   ++  PR+++
Sbjct: 128619 GAVLRVPCRRSSRLCVVVAAPRRADAAVATHGVFVHFFDFKLIHGYNRDGCLVSSPRLLQ 128440

Query: 375    VAPQVATQ 382
              +APQ A++
Sbjct: 128439 LAPQRASE 128416


  Database: S.parasitica/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 53,132,636
  Number of sequences in database:  1445
  
Lambda     K      H
   0.320    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,899,408
Number of Sequences: 1445
Number of extensions: 386841
Number of successful extensions: 1732
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 45
Number of HSP's that attempted gapping in prelim test: 1355
Number of HSP's gapped (non-prelim): 763
length of query: 392
length of database: 17,710,878
effective HSP length: 106
effective length of query: 286
effective length of database: 17,557,708
effective search space: 5021504488
effective search space used: 5021504488
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 64 (29.3 bits)