TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: S.parasitica/genome.fa 
           1445 sequences; 53,132,636 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...   102   5e-45
gb|GG743964.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    32   1.2  
gb|GG743992.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    30   6.2  

>gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.24, whole
              genome shotgun sequence
          Length = 471063

 Score =  102 bits (254), Expect(4) = 5e-45
 Identities = 72/196 (36%), Positives = 101/196 (51%), Gaps = 34/196 (17%)
 Frame = +3

Query: 89     GRIALANVLSDVYAVGVTQFDTVEMIVSTSTSFSEKERDVVIGLVMKGFQNSLKANGYRN 148
              GRIA ANVLSD+YA+GVT+ DT+ MI+  S + ++ ERDVV   ++ GF +  +  G   
Sbjct: 142827 GRIACANVLSDLYAMGVTEVDTMLMILGVSRTMTDLERDVVTTQLIHGFNDLAREAG--- 142997

Query: 149    TPLIIRQLKINPWCIIGGIA-----------------TSVCRSEEIILPSNAQPGDVLVL 191
              T +   Q  +NPW I+GG+A                 T++    EII P NA  G +LVL
Sbjct: 142998 TNVTGGQTVMNPWPIVGGVAMSVRAGSPMFSSTKRNLTNIHLESEIIRPENATAGQMLVL 143177

Query: 192    TKPLGGQMAM---------DAHLWQL--------NQTEKYKKLLSECSDADIKETFEIAV 234
              TKPLG  + +          AH  QL        +Q  ++ K+ S  +       F +A 
Sbjct: 143178 TKPLGCALVLGGEFIASCCHAHSTQLAVNAYQMKDQPARWSKVESFLTREMADRAFSVAS 143357

Query: 235    KSMTYLNKNAALLMHK 250
              +SM+ LN N A LMHK
Sbjct: 143358 ESMSRLNVNGAKLMHK 143405



 Score = 57.8 bits (138), Expect(4) = 5e-45
 Identities = 34/90 (37%), Positives = 47/90 (52%), Gaps = 19/90 (21%)
 Frame = +2

Query: 256    ATDITGFGLLGHAN-------------------NLAQFQKEKVLFQINKLPIIKNVLKFS 296
              ATD+TGFG+LGHA                    N+A  QK  V F+++ LP+IK++L  +
Sbjct: 143471 ATDVTGFGILGHAQYGNTIGV*LLLTLAFAAARNMATSQKADVDFELHTLPVIKDMLAVN 143650

Query: 297    TLVGQSTKFRSGRSVETSGGLLICLPADAA 326
                     +   G S ETSGGLL+ + AD A
Sbjct: 143651 EAFNGQWRLELGFSSETSGGLLVSIDADRA 143740



 Score = 43.5 bits (101), Expect(4) = 5e-45
 Identities = 20/47 (42%), Positives = 27/47 (57%)
 Frame = +1

Query: 2      FQPEKHGLEPDFQLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNND 48
              F P  HGL  DF+LT F+   G  CK+PQ+ L  Y+ G   + K N+
Sbjct: 142483 FNPTDHGLREDFRLTDFSALKG*GCKLPQEKLLSYISGIANDLKPNE 142623



 Score = 37.7 bits (86), Expect(4) = 5e-45
 Identities = 17/33 (51%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
 Frame = +2

Query: 55     GMDCAVIPLKRHK--DYLLIQTVDFFYPMVNDP 85
              GMD +V+ +K  K  D  +I T DFFYP+V DP
Sbjct: 142676 GMDSSVVKIKHPKFGDLYMISTTDFFYPLVQDP 142774


>gb|GG743964.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.82, whole
             genome shotgun sequence
          Length = 147639

 Score = 32.0 bits (71), Expect = 1.2
 Identities = 21/69 (30%), Positives = 34/69 (49%)
 Frame = +3

Query: 60    VIPLKRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTST 119
             V+P+KR ++       D  +   N   L GR+ALA+ L+  Y   +     V + VS   
Sbjct: 11544 VVPIKRDRERS--SDHDLSHTATNQLALFGRVALASFLAHRYPKRLADLFLVHLGVSMCG 11717

Query: 120   SFSEKERDV 128
             SF+E++  V
Sbjct: 11718 SFNERQLGV 11744


>gb|GG743992.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.110,
            whole genome shotgun sequence
          Length = 106235

 Score = 29.6 bits (65), Expect = 6.2
 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
 Frame = -1

Query: 85   PELLGRIALANVLSDVYAVG--VTQFDTVEMIVSTSTSFSEKERDVVIGLVMKGF 137
            PELL R    N  +D+YA G  +T+ DT+E+  ++ T   +  R +  G +   F
Sbjct: 6794 PELLDRHGSYNAAADMYAFGALLTELDTLELPFASETVSDDIRRKLSEGSLRPHF 6630


  Database: S.parasitica/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 53,132,636
  Number of sequences in database:  1445
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,553,385
Number of Sequences: 1445
Number of extensions: 254271
Number of successful extensions: 1093
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 35
Number of HSP's that attempted gapping in prelim test: 880
Number of HSP's gapped (non-prelim): 414
length of query: 370
length of database: 17,710,878
effective HSP length: 105
effective length of query: 265
effective length of database: 17,559,153
effective search space: 4653175545
effective search space used: 4653175545
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)