TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000047_1.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Drosophila melanogaster # Complete
         (398 letters)

Database: S.parasitica/genome.fa 
           1445 sequences; 53,132,636 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...   160   6e-55
gb|GG744697.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    31   3.0  
gb|GG743913.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    30   5.2  

>gb|GG743906.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.24, whole
              genome shotgun sequence
          Length = 471063

 Score =  160 bits (406), Expect(3) = 6e-55
 Identities = 92/193 (47%), Positives = 117/193 (60%), Gaps = 35/193 (18%)
 Frame = +3

Query: 122    GKIACANVLSDLYAMGVTDCDNMLMLLAVSTKMTEKERDVVIPLIMRGFKDSALEAGTTV 181
              G+IACANVLSDLYAMGVT+ D MLM+L VS  MT+ ERDVV   ++ GF D A EAGT V
Sbjct: 142827 GRIACANVLSDLYAMGVTEVDTMLMILGVSRTMTDLERDVVTTQLIHGFNDLAREAGTNV 143006

Query: 182    TGGQSVVNPWCTIGGVA-----------------STICQPNEYIVPDNAVVGDVLVLTKP 224
              TGGQ+V+NPW  +GGVA                 + I   +E I P+NA  G +LVLTKP
Sbjct: 143007 TGGQTVMNPWPIVGGVAMSVRAGSPMFSSTKRNLTNIHLESEIIRPENATAGQMLVLTKP 143186

Query: 225    LG------------------TQVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMNSM 266
              LG                  TQ+AVNA+Q  DQP RW++++  ++ E   +A+  A  SM
Sbjct: 143187 LGCALVLGGEFIASCCHAHSTQLAVNAYQMKDQPARWSKVESFLTREMADRAFSVASESM 143366

Query: 267    ARLNRVAARLMHK 279
              +RLN   A+LMHK
Sbjct: 143367 SRLNVNGAKLMHK 143405



 Score = 71.2 bits (173), Expect(2) = 2e-14
 Identities = 47/148 (31%), Positives = 75/148 (50%), Gaps = 23/148 (15%)
 Frame = +2

Query: 231    VNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMNSMARLNRVAARLMHKYNA-HG---AT 286
              V   +++D  +   R++  +  ++  +   R  ++   +   ++  MH  +A HG   AT
Sbjct: 143297 VQGRKFLDAGDGRPRVQRRIRVDEPTQRQRRQAHAQGIITTCSSGNMHTDHA*HGVTSAT 143476

Query: 287    DITGFGLLGHAQ-------------------TLAAHQKKDVSFVIHNLPVIAKMAAVAKA 327
              D+TGFG+LGHAQ                    +A  QK DV F +H LPVI  M AV +A
Sbjct: 143477 DVTGFGILGHAQYGNTIGV*LLLTLAFAAARNMATSQKADVDFELHTLPVIKDMLAVNEA 143656

Query: 328    CGNMFQLLQGHSAETSGGLLICLPREQA 355
                  ++L  G S+ETSGGLL+ +  ++A
Sbjct: 143657 FNGQWRLELGFSSETSGGLLVSIDADRA 143740



 Score = 50.1 bits (118), Expect(3) = 6e-55
 Identities = 23/45 (51%), Positives = 29/45 (64%)
 Frame = +1

Query: 24     RRPFDPTAHDLDASFRLTRFADLKGRGCKVPQDVLSKLVSALQQD 68
              R PF+PT H L   FRLT F+ LKG GCK+PQ+ L   +S +  D
Sbjct: 142474 RAPFNPTDHGLREDFRLTDFSALKG*GCKLPQEKLLSYISGIAND 142608



 Score = 43.9 bits (102), Expect(3) = 6e-55
 Identities = 18/35 (51%), Positives = 26/35 (74%), Gaps = 3/35 (8%)
 Frame = +2

Query: 89     GLDCSVIPLRH---GGLCLVQTTDFFYPIVDDPYM 120
              G+D SV+ ++H   G L ++ TTDFFYP+V DPY+
Sbjct: 142676 GMDSSVVKIKHPKFGDLYMISTTDFFYPLVQDPYV 142780



 Score = 26.9 bits (58), Expect(2) = 2e-14
 Identities = 12/38 (31%), Positives = 20/38 (52%)
 Frame = +1

Query: 357    AYCKDIEKQEGYQAWIIGIVEKGNKTARIIDKPRVIEV 394
              A+  D+   +   AWI+G V +G K AR+      +E+
Sbjct: 143788 AFIDDLVALDMQPAWIVGRVVQGTKQARLAPDVTFVEI 143901


>gb|GG744697.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.815,
            whole genome shotgun sequence
          Length = 3158

 Score = 30.8 bits (68), Expect = 3.0
 Identities = 15/44 (34%), Positives = 27/44 (61%)
 Frame = +3

Query: 194  IGGVASTICQPNEYIVPDNAVVGDVLVLTKPLGTQVAVNAHQWI 237
            IG V  +IC   EY+     V+G+ +V+T+ LG ++A N+ + +
Sbjct: 3021 IGTVVHSICV-EEYVDGVTVVIGNFVVVTRELGPEIAQNSRKLV 3149


>gb|GG743913.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.31, whole
              genome shotgun sequence
          Length = 358087

 Score = 30.0 bits (66), Expect = 5.2
 Identities = 31/120 (25%), Positives = 52/120 (43%), Gaps = 4/120 (3%)
 Frame = -2

Query: 242    RWNRIKLVVSEEDVRKAY-HRAMNSMARLN---RVAARLMHKYNAHGATDITGFGLLGHA 297
              R  R++ V     VR+ +  R   ++A ++      A L+H  +A  A  +   GL    
Sbjct: 118881 RRRRVRRVRWRHRVRRGHVGRVRTTLAHVHTHVHAVAHLLHLGHAVHAHHVVAMGLAVVR 118702

Query: 298    QTLAAHQKKDVSFVIHNLPVIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAA 357
              + +AAH     +  +H   V A +AAVA    +    + GH+A    G ++   R   AA
Sbjct: 118701 RHVAAHAGH--AHRVHGRGVAAAVAAVAAGHAHAHAHVHGHAAVRHAGHVVHAGRVHHAA 118528


  Database: S.parasitica/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 53,132,636
  Number of sequences in database:  1445
  
Lambda     K      H
   0.321    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,132,213
Number of Sequences: 1445
Number of extensions: 406794
Number of successful extensions: 1999
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 55
Number of HSP's that attempted gapping in prelim test: 1491
Number of HSP's gapped (non-prelim): 965
length of query: 398
length of database: 17,710,878
effective HSP length: 106
effective length of query: 292
effective length of database: 17,557,708
effective search space: 5126850736
effective search space used: 5126850736
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 64 (29.3 bits)