TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000016_1.0 # Protein # Selenoprotein K (SelK) # Homo sapiens # Complete (94 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000618.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 27 1.7 gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 26 3.8 gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 25 8.4 >gb|CM000618.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 16, whole genome shotgun sequence Length = 764225 Score = 26.9 bits (58), Expect = 1.7 Identities = 16/50 (32%), Positives = 28/50 (56%) Frame = -2 Query: 11 DSRSQSPWRLSLITDFFWGIAEFVVLFFKTLLQQDVKKRRSYGNSSDSRY 60 D +S + WR++++ DFF +E V +++ K RR G SS+ +Y Sbjct: 216583 DKQSLASWRVAMVADFF-DESEIVP------MERGPKGRR*LGTSSNCKY 216455 >gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 23, whole genome shotgun sequence Length = 512847 Score = 25.8 bits (55), Expect = 3.8 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = +2 Query: 7 GQVLDSRSQSPWRLSLITDFFWGI 30 G V S S S S ITDF+WG+ Sbjct: 109307 GHVAPSSSDSGTNGSWITDFYWGM 109378 >gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun sequence Length = 2535400 Score = 24.6 bits (52), Expect = 8.4 Identities = 8/25 (32%), Positives = 17/25 (68%) Frame = +3 Query: 9 VLDSRSQSPWRLSLITDFFWGIAEF 33 V+ +++++PW + L T + WG+ F Sbjct: 1867482 VIMTKAKNPWTVRLETTWRWGLRAF 1867556 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.325 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,830,651 Number of Sequences: 31 Number of extensions: 17513 Number of successful extensions: 126 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 121 Number of HSP's gapped (non-prelim): 9 length of query: 94 length of database: 7,911,228 effective HSP length: 69 effective length of query: 25 effective length of database: 7,909,089 effective search space: 197727225 effective search space used: 197727225 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 51 (24.3 bits)