TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000055_1.0 # Protein # Selenophosphate synthetase 1 (SPS1) # Anopheles gambiae # Complete (396 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000622.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 75 8e-14 gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 6.9 >gb|CM000622.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 20, whole genome shotgun sequence Length = 683011 Score = 74.7 bits (182), Expect = 8e-14 Identities = 62/206 (30%), Positives = 94/206 (45%), Gaps = 3/206 (1%) Frame = -2 Query: 92 VIPLRHGGLCMVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTEVDNMLMLLAVSTK 151 ++P + GG +VQT D+F IV DP+ GKI + LSD++AMG T M LAV+ Sbjct: 128631 ILPNKAGG-AIVQTIDYFREIVKDPFTFGKIVAVHALSDIHAMGAT--PQTAMTLAVAPF 128461 Query: 152 MIEKE-RDVVIPLIMRGFKDSALEAGTSVTGGHSVVNPWCTIGGVATTICQQNEFIV-PD 209 ++E + + ++ G D + + GGH+ G + ++ Sbjct: 128460 AADEEVTESTLLHLLSGVSDILQDENVQLVGGHTCEGLELACGLSVQGYTDNPKLLLRKQ 128281 Query: 210 NAVVGDVLVLTKALGTQVAVNAHQWLDQSERWNRIKLVVSEEDVRKAYHRAMDSMSRLNR 269 +GD +VLTK +GT L ++ R K E A+DSM N Sbjct: 128280 GGAIGDKIVLTKPIGTGA-------LFAADMRARCKGSYMSE--------ALDSMIHSNC 128146 Query: 270 VAARL-MHKYNAHGATDVTGFGLLGH 294 A+++ M TDVTGFGL+GH Sbjct: 128145 HASQVAMRAKGIRSCTDVTGFGLIGH 128068 >gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 18, whole genome shotgun sequence Length = 702471 Score = 28.5 bits (62), Expect = 6.9 Identities = 18/44 (40%), Positives = 23/44 (52%) Frame = +3 Query: 24 RPFDPTAHDLDASFRLTRFADLKGRCCKVPKEVLEKLVSSLQQD 67 RP T +LD FR+ R R VPK + KLVS+ Q+D Sbjct: 145806 RPETATVDNLDVGFRVER------RITIVPKTWIVKLVSNAQKD 145919 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.322 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,122,205 Number of Sequences: 31 Number of extensions: 167895 Number of successful extensions: 771 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 765 Number of HSP's gapped (non-prelim): 18 length of query: 396 length of database: 7,911,228 effective HSP length: 100 effective length of query: 296 effective length of database: 7,908,128 effective search space: 2340805888 effective search space used: 2340805888 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 61 (28.1 bits)