TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000016_1.0 # Protein # Selenoprotein K (SelK) # Homo
sapiens # Complete
         (94 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738900.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   2.9  
gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    26   5.0  

>gb|GG738900.1| Naegleria gruberi genomic scaffold NAEGRscaffold_56, whole genome shotgun
              sequence
          Length = 312004

 Score = 26.9 bits (58), Expect = 2.9
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = +3

Query: 3      YISNGQVLDSRSQSPWRLSLITDFFWGIAEFVVLFFKTLLQQDVKK 48
              YIS+  +  S S S W L L    FWG      LFF  L+ + +++
Sbjct: 185445 YISSHGLRFSFSGSSWNLYLCWSKFWGSMVNHFLFFSILVLEYLRR 185582


>gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome shotgun
              sequence
          Length = 1001995

 Score = 26.2 bits (56), Expect = 5.0
 Identities = 14/39 (35%), Positives = 23/39 (58%)
 Frame = -3

Query: 19     RLSLITDFFWGIAEFVVLFFKTLLQQDVKKRRSYGNSSD 57
              R  +I +  W +   +VL    +L QDVK+R S+GN ++
Sbjct: 752237 RSKIINNHSWCVVIKMVLLL--VLIQDVKERHSHGNQNN 752127


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.325    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,127,767
Number of Sequences: 784
Number of extensions: 31657
Number of successful extensions: 194
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 152
Number of HSP's gapped (non-prelim): 67
length of query: 94
length of database: 13,654,695
effective HSP length: 69
effective length of query: 25
effective length of database: 13,600,599
effective search space: 340014975
effective search space used: 340014975
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)