TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000044_1.0 # Protein # Selenoprotein K1 (SelK1) #
Drosophila melanogaster # Complete
         (110 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   2.9  
gb|GG738857.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    26   5.0  
gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    25   8.5  

>gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_14, whole genome shotgun
              sequence
          Length = 782252

 Score = 26.9 bits (58), Expect = 2.9
 Identities = 11/38 (28%), Positives = 20/38 (52%), Gaps = 7/38 (18%)
 Frame = -1

Query: 6      HNGRVWEKRPWDWRRIVELF-------VGIWFAIKQLF 36
              + G++W K  + WR +++LF       + +WF  K  F
Sbjct: 759545 YQGQLWNKGRFLWRCLIQLFNKELFSMITMWFLFKGFF 759432


>gb|GG738857.1| Naegleria gruberi genomic scaffold NAEGRscaffold_13, whole genome shotgun
              sequence
          Length = 573072

 Score = 26.2 bits (56), Expect = 5.0
 Identities = 11/16 (68%), Positives = 11/16 (68%)
 Frame = +2

Query: 24     LFVGIWFAIKQLFLTF 39
              LFVGIWF I Q  L F
Sbjct: 529151 LFVGIWFVILQRILVF 529198


>gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_61, whole genome shotgun
              sequence
          Length = 290827

 Score = 25.4 bits (54), Expect = 8.5
 Identities = 12/35 (34%), Positives = 16/35 (45%)
 Frame = -3

Query: 9      RVWEKRPWDWRRIVELFVGIWFAIKQLFLTFLAPF 43
              R W KR      I+  F G    +   F+T +APF
Sbjct: 198986 RCWSKRVCHNLWILLFFFGFGLTLYSFFITIIAPF 198882


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.328    0.144    0.508 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,191,780
Number of Sequences: 784
Number of extensions: 42881
Number of successful extensions: 299
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 256
Number of HSP's gapped (non-prelim): 90
length of query: 110
length of database: 13,654,695
effective HSP length: 85
effective length of query: 25
effective length of database: 13,588,055
effective search space: 339701375
effective search space used: 339701375
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)