TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|70870239|gb|AAHK01001632.1|:subseq(1423,2260) Trypanosoma
cruzi strain CL Brener tcruzi_1047053508727, whole genome shotgun
sequence:[translate(1)]
         (89 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738862.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   3.8  
gb|GG738887.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    26   5.0  
gb|GG738950.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    26   6.5  
gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    26   6.5  
gb|GG738890.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    25   8.5  
gb|GG738885.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    25   8.5  
gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    25   8.5  

>gb|GG738862.1| Naegleria gruberi genomic scaffold NAEGRscaffold_18, whole genome shotgun
              sequence
          Length = 502116

 Score = 26.6 bits (57), Expect = 3.8
 Identities = 13/32 (40%), Positives = 22/32 (68%)
 Frame = +3

Query: 9      VVDSKPFSLVDFFLSILRLMWLFFATLVSSEP 40
              ++ + P SLV FFL +L+++ +FF+  VS  P
Sbjct: 478830 IISNSP-SLV-FFLKVLKILTIFFSASVSERP 478919


>gb|GG738887.1| Naegleria gruberi genomic scaffold NAEGRscaffold_43, whole genome
             shotgun sequence
          Length = 508457

 Score = 26.2 bits (56), Expect = 5.0
 Identities = 13/25 (52%), Positives = 15/25 (60%)
 Frame = -1

Query: 15    FSLVDFFLSILRLMWLFFATLVSSE 39
             F+  DFF  I+RL W   ATL S E
Sbjct: 76589 FN*FDFFFEIVRLFWRS*ATLQSRE 76515


>gb|GG738950.1| Naegleria gruberi genomic scaffold NAEGRscaffold_106, whole genome
            shotgun sequence
          Length = 20111

 Score = 25.8 bits (55), Expect = 6.5
 Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 1/59 (1%)
 Frame = +3

Query: 30   LFFATLVSSEPVSQ-HVQEYRSTTRIVPFGGGRSVGHAPRGPNIHGLPKGSLTGGCATG 87
            +FF+TL   E  S  +         IV   GGR +G    G N  G+P G L+   A G
Sbjct: 9327 MFFSTLDKGEMKSMVYNWPVEEVDVIVVTDGGRILGLGDLGANGDGIPIGKLSLYVAAG 9503


>gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_7, whole genome shotgun
              sequence
          Length = 693645

 Score = 25.8 bits (55), Expect = 6.5
 Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 6/42 (14%)
 Frame = +3

Query: 3      YVANGRVVDSKPFSLVDFFLSILRL------MWLFFATLVSS 38
              +VANG +++  PF +V  F  IL L      M  +F  LVSS
Sbjct: 583065 WVANGSIMNLFPFGVVSMFERILFLNLSIIEMDSYFGFLVSS 583190


>gb|GG738890.1| Naegleria gruberi genomic scaffold NAEGRscaffold_46, whole genome shotgun
              sequence
          Length = 350200

 Score = 25.4 bits (54), Expect = 8.5
 Identities = 15/40 (37%), Positives = 21/40 (52%)
 Frame = -1

Query: 9      VVDSKPFSLVDFFLSILRLMWLFFATLVSSEPVSQHVQEY 48
              VVDSK  S ++F +         F T ++S    QHVQ+Y
Sbjct: 105337 VVDSKVGSEIEFTMLTTNNYHDNFPTTITSPHHHQHVQQY 105218


>gb|GG738885.1| Naegleria gruberi genomic scaffold NAEGRscaffold_41, whole genome
             shotgun sequence
          Length = 362555

 Score = 25.4 bits (54), Expect = 8.5
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = -1

Query: 2     PYVANGRVVDSKPFSLVDFFLSILRLMWLF 31
             P  +NG VVDS   +L+ F + + +L+ LF
Sbjct: 51623 PLESNGLVVDSSSQALIIFMVDL*KLLVLF 51534


>gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome
             shotgun sequence
          Length = 1001995

 Score = 25.4 bits (54), Expect = 8.5
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +1

Query: 15    FSLVDFFLSILRLMWLFFATLVSSEPVSQH 44
             F+  D FL+ +   WLFF++ +S E +  H
Sbjct: 69559 FNSKDHFLNFIPGKWLFFSSHLSLELIVNH 69648


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.323    0.141    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,967,023
Number of Sequences: 784
Number of extensions: 47415
Number of successful extensions: 279
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 67
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 192
Number of HSP's gapped (non-prelim): 144
length of query: 89
length of database: 13,654,695
effective HSP length: 64
effective length of query: 25
effective length of database: 13,604,519
effective search space: 340112975
effective search space used: 340112975
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)