TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000047_1.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Drosophila melanogaster # Complete
         (398 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   122   5e-28
gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   109   5e-24
gb|GG738856.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    30   3.1  
gb|GG738873.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    29   9.0  

>gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_8, whole genome shotgun
              sequence
          Length = 938756

 Score =  122 bits (307), Expect = 5e-28
 Identities = 109/378 (28%), Positives = 166/378 (43%), Gaps = 38/378 (10%)
 Frame = +1

Query: 39     RLTRFADLKGRGCKVPQDVLSKLVSALQQDYSAQDQEPQFLNVAIPRIGIGL--DCSVIP 96
              +LT      G GCK+    LS ++S + +    Q  +P  L      +G     DC V  
Sbjct: 697966 KLTSTTHGLGCGCKIRAQTLSSILSKIPK----QPFDPNVL------VGTETSDDCGVYK 698115

Query: 97     LRHGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLLAVSTKMTE 156
              L    L L  T DFF PIVD P   G IACAN LSD+YAMG  +C   L ++A   K   
Sbjct: 698116 LT-SELALCATVDFFTPIVDSPKDFGAIACANALSDVYAMG-GECKVALNIVAFPVKRLS 698289

Query: 157    KERDVVIPLIMRGFKDSALEAGTTVTGGQSVVNPWCTIGGVASTICQPNEYIVPDNAVVG 216
               +   ++ LI++G ++ A EAG T+ GG S+ +     G   S +  P           G
Sbjct: 698290 LD---ILQLILQGAQEKASEAGVTIIGGHSIEDNEPKFGMCVSGLVHPERIWKNVGCRNG 698460

Query: 217    DVLVLTKPLGTQVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMNSMARLNRVAARL 276
              DVL+LTKP+GT V   A           +  L+   +D  K   + +  M+ LN+ A+ +
Sbjct: 698461 DVLLLTKPIGTGVLTTA----------MKKGLIGPNDDCSK---KCIEWMSTLNKSASEI 698601

Query: 277    MH----------KYNAHGATDITGFGLLGHAQTLAAHQKKD----------VSFV----- 311
              +            Y  H  TDITGFGL+GH + +     +D          V F+     
Sbjct: 698602 LRGLHRVNSSSTPYEIHACTDITGFGLMGHLREMITGDNQDNLYAQIESEKVPFMDKAYE 698781

Query: 312    -----------IHNLPVIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCK 360
                         I+N+  + K+    ++   + + L    A+TSGGLL  +   ++     
Sbjct: 698782 MILSDIIPGGTINNMNFVQKITKFNESVSQIVRTLL-CDAQTSGGLLFSVSPHESEEIIS 698958

Query: 361    DIEKQEGYQAWIIGIVEK 378
              D  K+ G   W +G +++
Sbjct: 698959 DF-KRLGISIWTVGSIKQ 699009


>gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_39, whole genome
             shotgun sequence
          Length = 371401

 Score =  109 bits (272), Expect = 5e-24
 Identities = 104/375 (27%), Positives = 164/375 (43%), Gaps = 33/375 (8%)
 Frame = -2

Query: 39    RLTRFADLKGRGCKVPQDVLSKLVSALQQDYSAQDQEPQFLNVAIPRIGIGLDCSVIPLR 98
             +LT+ A   G GCK+   +L  ++  L +       +P+  +V +       D +V  + 
Sbjct: 75705 KLTQLASGLGCGCKINSRLLDSILQKLPK-------QPENNSVLVGNSTCD-DAAVYKI- 75553

Query: 99    HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLLAVSTKMTEKE 158
             +  + +V T DFF PIVD+PY  G IACAN LSD+YAMG       L ++A  ++    E
Sbjct: 75552 NDHVAIVSTIDFFAPIVDNPYDFGAIACANALSDVYAMGGKP-QTALSVVAFPSRTLPIE 75376

Query: 159   RDVVIPLIMRGFKDSALEAGTTVTGGQSVVNPWCTIGGVASTICQPNEYIVPDNAVVGDV 218
                ++  I+RG  D A EAG  + GG S+ +     G   + I  P++         GD+
Sbjct: 75375 ---ILQEILRGSIDKANEAGVAIVGGHSIDDEEPKFGMSVNGIIHPDKIFRNVGCEEGDL 75205

Query: 219   LVLTKPLGTQVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMNSMARLNRVAARLMH 278
             L LTK +GT V  +A +  + P                +    A+NSM  LNR  + ++ 
Sbjct: 75204 LFLTKRIGTGVITSAMKLGNVPYE-------------SETAQSAINSMKTLNRDTSYVLR 75064

Query: 279   KY-NAHGATDITGFGLLGHAQTL------AAHQKKDVSFVIH------------------ 313
             +  +    TD+TGFGLLGH + +         + KD+   ++                  
Sbjct: 75063 EMKSVSSCTDVTGFGLLGHLKEIMKGSREICEENKDLVAQLNAKQIPLLDSSCLSLMRIV 74884

Query: 314   -------NLPVIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQE 366
                    NL  + +    A       QL+    A+TSGGLL  +  ++         K++
Sbjct: 74883 IPGGTKTNLENVQQFVEFASTLSKEEQLILA-DAQTSGGLLFTISPDEEEKLVNSF-KEK 74710

Query: 367   GYQAWIIG-IVEKGN 380
                 W IG IV+K N
Sbjct: 74709 NLNLWKIGEIVKKPN 74665


>gb|GG738856.1| Naegleria gruberi genomic scaffold NAEGRscaffold_12, whole genome shotgun
              sequence
          Length = 602266

 Score = 30.4 bits (67), Expect = 3.1
 Identities = 42/173 (24%), Positives = 65/173 (37%), Gaps = 21/173 (12%)
 Frame = -1

Query: 62     VSALQQDYSAQDQEPQFLNVAIPRIGIGLDCSVIPLRHGGLCLVQTTDFFYPIVDDPYMM 121
              ++ L+QD    ++E  FL     R  + L    +  + GG+  +  T++   +   P   
Sbjct: 348250 INELEQDSKYNEEEDDFLQQIKARRLLELKQKQMATKFGGVQEISATEYVKEVCQTP--- 348080

Query: 122    GKIACANVLSDLYAMGVTDC---DNMLMLLA---VSTKMTEKERDVVIP----------L 165
                     V+  LYA  + DC   D+ L  L+   +  K         IP          L
Sbjct: 348079 --DKTTFVVVHLYAPAIEDCKILDDRLTKLSNKFLEVKFVRIRGSAAIPNFPEKNCPTLL 347906

Query: 166    IMRGFKDSALEAGTTVTGGQSVVN---PW--CTIGGVASTICQPNEYIVPDNA 213
              I RG  + A   G    GG+ +      W   TIG V S + QP    V  N+
Sbjct: 347905 IYRGGNNVAQFVGLGKIGGREMTANDLEWILSTIGVVKSEMKQPPSSRVAGNS 347747


>gb|GG738873.1| Naegleria gruberi genomic scaffold NAEGRscaffold_29, whole genome
             shotgun sequence
          Length = 461873

 Score = 28.9 bits (63), Expect = 9.0
 Identities = 21/66 (31%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
 Frame = -1

Query: 275   RLMHKYNAHGATDITGFGLLGH--AQTLAAH---QKKDVSFVIHNLPVIAKMAAVAKACG 329
             R M + NA   T++T   ++    A+TL           SFV+  + VI ++ A+AK CG
Sbjct: 55676 RKMKEVNALSTTNVTALAIIMESIAKTLLVII**TPTVHSFVMVTVLVIIRITALAKTCG 55497

Query: 330   NMFQLL 335
              +F +L
Sbjct: 55496 QIFTVL 55479


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.321    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,948,678
Number of Sequences: 784
Number of extensions: 213123
Number of successful extensions: 689
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 612
Number of HSP's gapped (non-prelim): 158
length of query: 398
length of database: 13,654,695
effective HSP length: 104
effective length of query: 294
effective length of database: 13,573,159
effective search space: 3990508746
effective search space used: 3990508746
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)