TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000016_1.0 # Protein # Selenoprotein K (SelK) # Homo sapiens # Complete (94 letters) Database: H.arabidopsidis/genome.fa 5422 sequences; 67,459,135 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABWE01000378.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 41 2e-04 >gb|ABWE01000378.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont29.9, whole genome shotgun sequence Length = 46228 Score = 41.2 bits (95), Expect = 2e-04 Identities = 18/38 (47%), Positives = 25/38 (65%) Frame = -1 Query: 1 MVYISNGQVLDSRSQSPWRLSLITDFFWGIAEFVVLFF 38 M Y++ G V+ +RS WRLS+I D FWG+ FV L + Sbjct: 40162 MTYVTEGNVVKTRSS--WRLSIIPDTFWGVLNFVGLLY 40055 Database: H.arabidopsidis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 67,459,135 Number of sequences in database: 5422 Lambda K H 0.325 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,870,673 Number of Sequences: 5422 Number of extensions: 45864 Number of successful extensions: 321 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 236 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 81 Number of HSP's gapped (non-prelim): 253 length of query: 94 length of database: 22,486,378 effective HSP length: 69 effective length of query: 25 effective length of database: 22,112,260 effective search space: 552806500 effective search space used: 552806500 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 55 (25.8 bits)