TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: E.siliculosus/genome.fa 
           35 sequences; 195,934,119 total letters

Searching...................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genom...    40   0.016
emb|FN649735.1| Ectocarpus siliculosus strain Ec 32, whole genom...    32   7.5  

>emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG02
          Length = 9289656

 Score = 40.4 bits (93), Expect = 0.016
 Identities = 20/50 (40%), Positives = 28/50 (56%)
 Frame = -1

Query: 116     GIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAM 165
               G+   + V P    G   V T DFF   V DP++ G+IA  + LSD++AM
Sbjct: 3477516 GLDDAAVVAPPSEPGAVTVHTVDFFRSFVSDPFVFGQIAANHALSDVHAM 3477367



 Score = 35.0 bits (79), Expect = 0.68
 Identities = 16/37 (43%), Positives = 22/37 (59%)
 Frame = -3

Query: 289     QEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGH 325
               Q A+ +M   N  AA ++    A A TD+TGFG+ GH
Sbjct: 3476047 QPAVRSMLQSNGPAATVLRDHGARACTDVTGFGVAGH 3475937


>emb|FN649735.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG10
          Length = 4527641

 Score = 31.6 bits (70), Expect = 7.5
 Identities = 16/50 (32%), Positives = 22/50 (44%)
 Frame = +1

Query: 61      GCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSP 110
               G ++P+      L G   P    P GR  V  +E++  EAG      PSP
Sbjct: 3966433 GSRLPRRCSTSSLPGANSPSGANPFGRAKVLSEEKSRSEAGANIRVSPSP 3966582


  Database: E.siliculosus/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 195,934,119
  Number of sequences in database:  35
  
Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,073,625
Number of Sequences: 35
Number of extensions: 1705305
Number of successful extensions: 9342
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 9148
Number of HSP's gapped (non-prelim): 570
length of query: 448
length of database: 65,311,373
effective HSP length: 116
effective length of query: 332
effective length of database: 65,307,313
effective search space: 21682027916
effective search space used: 21682027916
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 69 (31.2 bits)