TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: E.siliculosus/genome.fa 
           35 sequences; 195,934,119 total letters

Searching...................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genom...    39   0.048
emb|FN649752.1| Ectocarpus siliculosus strain Ec 32, whole genom...    36   0.24 
emb|FN649740.1| Ectocarpus siliculosus strain Ec 32, whole genom...    32   5.8  

>emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG02
          Length = 9289656

 Score = 38.5 bits (88), Expect = 0.048
 Identities = 19/47 (40%), Positives = 28/47 (59%)
 Frame = -1

Query: 57      DCAVIPLKRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAV 103
               D AV+        + + TVDFF   V+DP + G+IA  + LSDV+A+
Sbjct: 3477507 DAAVVAPPSEPGAVTVHTVDFFRSFVSDPFVFGQIAANHALSDVHAM 3477367



 Score = 35.8 bits (81), Expect = 0.31
 Identities = 16/35 (45%), Positives = 22/35 (62%)
 Frame = -3

Query: 233     AVKSMTYLNKNAALLMHKYQAHCATDITGFGLLGH 267
               AV+SM   N  AA ++  + A   TD+TGFG+ GH
Sbjct: 3476041 AVRSMLQSNGPAATVLRDHGARACTDVTGFGVAGH 3475937


>emb|FN649752.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG27
          Length = 4309624

 Score = 36.2 bits (82), Expect = 0.24
 Identities = 23/65 (35%), Positives = 36/65 (55%), Gaps = 1/65 (1%)
 Frame = +1

Query: 27      KIPQKVLEKYLR-GTEIENKNNDGYLIGSGMDCAVIPLKRHKDYLLIQTVDFFYPMVNDP 85
               ++P K L +YL+     E K NDG L+G G+D A   +++ +  LL+  + FF  +V  P
Sbjct: 2458123 RVPSKTLPQYLKYDRTTEGKMNDGGLVGRGIDLA--KMRQKRTLLLVVCLWFF*ILVRVP 2458296

Query: 86      ELLGR 90
                 L R
Sbjct: 2458297 *WLAR 2458311


>emb|FN649740.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG15
          Length = 5788546

 Score = 31.6 bits (70), Expect = 5.8
 Identities = 15/55 (27%), Positives = 27/55 (49%)
 Frame = +2

Query: 125     ERDVVIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSEEIIL 179
               ++ VV G++  GFQ           PL+I   +++ WC + G  T  CR   +++
Sbjct: 4011242 QQAVVHGVLYAGFQTEQLQCWGSARPLLI*SGRVDGWCCLAGAETDSCRVAMVVI 4011406


  Database: E.siliculosus/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 195,934,119
  Number of sequences in database:  35
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,222,583
Number of Sequences: 35
Number of extensions: 1073186
Number of successful extensions: 4587
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4491
Number of HSP's gapped (non-prelim): 294
length of query: 370
length of database: 65,311,373
effective HSP length: 114
effective length of query: 256
effective length of database: 65,307,383
effective search space: 16718690048
effective search space used: 16718690048
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)