TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Anopheles gambiae # Complete
         (369 letters)

Database: E.siliculosus/genome.fa 
           35 sequences; 195,934,119 total letters

Searching...................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genom...    41   0.007
emb|FN649736.1| Ectocarpus siliculosus strain Ec 32, whole genom...    32   5.8  
emb|FN649756.1| Ectocarpus siliculosus strain Ec 32, whole genom...    32   5.8  
emb|FN649737.1| Ectocarpus siliculosus strain Ec 32, whole genom...    31   9.9  

>emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG02
          Length = 9289656

 Score = 41.2 bits (95), Expect = 0.007
 Identities = 29/89 (32%), Positives = 42/89 (47%), Gaps = 3/89 (3%)
 Frame = -1

Query: 23      GUGSKVPQDVLNRLLAGVYGEQLGDKDGKGPNKEEGVGIGLDSSVIAL---KHDLFLVQS 79
               G GSKV   VL R+L+ +                 GV  GLD + +     +     V +
Sbjct: 3477582 GCGSKVGAGVLGRVLSRL-----------------GVAGGLDDAAVVAPPSEPGAVTVHT 3477454

Query: 80      VDFFYPLIDDPFMLGKIALANVVSDVFAV 108
               VDFF   + DPF+ G+IA  + +SDV A+
Sbjct: 3477453 VDFFRSFVSDPFVFGQIAANHALSDVHAM 3477367



 Score = 38.5 bits (88), Expect = 0.047
 Identities = 17/35 (48%), Positives = 24/35 (68%)
 Frame = -3

Query: 236     ALESMSRLNKTGAELMKKYGAHAATDVTGFGLYGH 270
               A+ SM + N   A +++ +GA A TDVTGFG+ GH
Sbjct: 3476041 AVRSMLQSNGPAATVLRDHGARACTDVTGFGVAGH 3475937


>emb|FN649736.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG11
          Length = 5521395

 Score = 31.6 bits (70), Expect = 5.8
 Identities = 29/108 (26%), Positives = 45/108 (41%), Gaps = 8/108 (7%)
 Frame = -1

Query: 255     GAHAATDVTGFGLYGHAENLASHQT-----ADVDFHLDTLPIIKNVREIAETLGRGAKLL 309
               G +A   V     YG++    +H T     A+    L TLP  K+        G+   L 
Sbjct: 4159341 GVYAGAAVWRCAAYGNSSG-GAHTTRVKLQANQHHELKTLPTHKD--------GQHYSLC 4159189

Query: 310     AGKAVETSGGLLICLPREAAAGFCEEYRRCTKHEAWIVG---HVEKGA 354
                GK VE  GG+ IC+ +   A     Y  C +   + V    +++KG+
Sbjct: 4159188 RGKIVEKEGGICICIWKREEAESSSTYAYCCERTRFQVNGGMNLQKGS 4159045


>emb|FN649756.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
             assembly, chromosome LG31
          Length = 577967

 Score = 31.6 bits (70), Expect = 5.8
 Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 7/58 (12%)
 Frame = +1

Query: 121   TAPTEFTEQEREVVVPMVMQGFLEAA-------KACNAPVQIGSIAENPWCVIGGAAS 171
             T+P + T   R ++  +    F+EAA       + CNA   +GS     +  +GG AS
Sbjct: 55561 TSPVDLTLARRGLIFNLCFGDFIEAAYPFMERCRCCNAGAYLGSALSGGFVAVGGVAS 55734


>emb|FN649737.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG12
          Length = 3842133

 Score = 30.8 bits (68), Expect = 9.9
 Identities = 18/69 (26%), Positives = 31/69 (44%)
 Frame = +2

Query: 157     SIAENPWCVIGGAASAVCHRSELIMPYNAQPGDALVLTKPLGTQLATNAYIWMGEQQSDS 216
               S++   WC +G       H        +AQ    L +T+PL   +++  ++  G+  +  
Sbjct: 1227224 SLSRPTWC-LGSPGHGSAHNKH---QPDAQQRQFLHVTRPLSGVISSGRWLSAGQLLAQK 1227391

Query: 217     WAQLRERFT 225
               W Q RER T
Sbjct: 1227392 WIQRRERLT 1227418


  Database: E.siliculosus/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 195,934,119
  Number of sequences in database:  35
  
Lambda     K      H
   0.319    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,617,227
Number of Sequences: 35
Number of extensions: 1246789
Number of successful extensions: 6283
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 6144
Number of HSP's gapped (non-prelim): 469
length of query: 369
length of database: 65,311,373
effective HSP length: 114
effective length of query: 255
effective length of database: 65,307,383
effective search space: 16653382665
effective search space used: 16653382665
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 68 (30.8 bits)