TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|70870239|gb|AAHK01001632.1|:subseq(1423,2260) Trypanosoma cruzi strain CL Brener tcruzi_1047053508727, whole genome shotgun sequence:[translate(1)] (89 letters) Database: C.owczarzaki/genome.fa 89 sequences; 28,043,798 total letters Searching........................................................................................done Score E Sequences producing significant alignments: (bits) Value supercontig_1.3 of Capsaspora owczarzaki ATCC 30864 31 0.14 supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 30 0.18 supercontig_1.12 of Capsaspora owczarzaki ATCC 30864 27 2.6 supercontig_1.1 of Capsaspora owczarzaki ATCC 30864 27 2.6 supercontig_1.19 of Capsaspora owczarzaki ATCC 30864 26 3.4 supercontig_1.14 of Capsaspora owczarzaki ATCC 30864 26 4.5 supercontig_1.10 of Capsaspora owczarzaki ATCC 30864 25 5.8 supercontig_1.7 of Capsaspora owczarzaki ATCC 30864 25 7.6 supercontig_1.6 of Capsaspora owczarzaki ATCC 30864 25 7.6 supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 25 7.6 supercontig_1.11 of Capsaspora owczarzaki ATCC 30864 25 9.9 supercontig_1.5 of Capsaspora owczarzaki ATCC 30864 25 9.9 >supercontig_1.3 of Capsaspora owczarzaki ATCC 30864 Length = 2526540 Score = 30.8 bits (68), Expect = 0.14 Identities = 13/28 (46%), Positives = 14/28 (50%) Frame = -1 Query: 57 FGGGRSVGHAPRGPNIHGLPKGSLTGGC 84 FG G P GP+ H LP GS T C Sbjct: 662058 FGRGHPWPRCPGGPSCHQLPSGSATSAC 661975 Score = 26.2 bits (56), Expect = 3.4 Identities = 13/26 (50%), Positives = 14/26 (53%) Frame = -3 Query: 64 GHAPRGPNIHGLPKGSLTGGCATGUR 89 G PR P H LPK SLT C + R Sbjct: 501841 GGCPRAPFAH*LPKPSLTVRCRSQVR 501764 Score = 25.8 bits (55), Expect = 4.5 Identities = 17/53 (32%), Positives = 28/53 (52%) Frame = +1 Query: 4 VANGRVVDSKPFSLVDFFLSILRLMWLFFATLVSSEPVSQHVQEYRSTTRIVP 56 ++ G V+ +PF FF +LFF L+S ++QHV+ S+T + P Sbjct: 573064 LSGGGVLLPRPF----FFC----FCFLFFFVLLSPSMLNQHVETECSSTTLTP 573198 Score = 24.6 bits (52), Expect = 9.9 Identities = 17/49 (34%), Positives = 22/49 (44%) Frame = +1 Query: 3 YVANGRVVDSKPFSLVDFFLSILRLMWLFFATLVSSEPVSQHVQEYRST 51 +V+ R V PFSL+ L ILRL +S +S H R T Sbjct: 2440954 FVSFFRFVSFHPFSLLLMLLLILRLSLPIILPCISFRFISFHFLSLRFT 2441100 >supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 Length = 2141673 Score = 30.4 bits (67), Expect = 0.18 Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 9/47 (19%) Frame = -2 Query: 52 TRIVPFGGGRSVGHAPRGPNIHGLPKGSL---------TGGCATGUR 89 TR +P G +V P GP HG P+GS+ T CAT R Sbjct: 162287 TRCLPTRPGAAVCKTPPGPPGHGFPRGSIQCR*RLGRRTLACATSVR 162147 Score = 25.4 bits (54), Expect = 5.8 Identities = 15/33 (45%), Positives = 16/33 (48%) Frame = +3 Query: 44 HVQEYRSTTRIVPFGGGRSVGHAPRGPNIHGLP 76 H Q R RIVP GGG + G N GLP Sbjct: 1996599 HHQRERLNDRIVPIGGGTTRGFG-TDDNDDGLP 1996694 Score = 25.0 bits (53), Expect = 7.6 Identities = 11/18 (61%), Positives = 13/18 (72%) Frame = -2 Query: 59 GGRSVGHAPRGPNIHGLP 76 GGRSVG A R P++ G P Sbjct: 846005 GGRSVGAAFRVPDLRGCP 845952 >supercontig_1.12 of Capsaspora owczarzaki ATCC 30864 Length = 1052152 Score = 26.6 bits (57), Expect = 2.6 Identities = 17/51 (33%), Positives = 19/51 (37%), Gaps = 9/51 (17%) Frame = +1 Query: 16 SLVDFFLSILRLMWLFF---------ATLVSSEPVSQHVQEYRSTTRIVPF 57 S +DFF S L WLFF TL P + TT PF Sbjct: 21946 SCLDFFASFLSFFWLFFFLFF*VILVGTLFVRRPRMMQNTSKKQTTARCPF 22098 Score = 26.2 bits (56), Expect = 3.4 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = -2 Query: 59 GGRSVGHAPRGPNIHGLPKGSLTGGCATG 87 GG VGH PR +HG G C +G Sbjct: 409614 GGLDVGHIPRVETMHGFGGEEGHGLCCSG 409528 >supercontig_1.1 of Capsaspora owczarzaki ATCC 30864 Length = 3794667 Score = 26.6 bits (57), Expect = 2.6 Identities = 10/13 (76%), Positives = 11/13 (84%) Frame = -1 Query: 54 IVPFGGGRSVGHA 66 I+PFGGG SV HA Sbjct: 2106519 IIPFGGGTSVSHA 2106481 Score = 25.8 bits (55), Expect = 4.5 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +1 Query: 20 FFLSILRLMWLFFATLVSSEPVSQHVQEYRSTTR 53 FFL I+ L WLFF S + SQ ++ R + Sbjct: 1913926 FFLIIVFL*WLFFFFFFSKKEFSQESRKQRKRAK 1914027 Score = 25.4 bits (54), Expect = 5.8 Identities = 21/61 (34%), Positives = 28/61 (45%), Gaps = 5/61 (8%) Frame = -3 Query: 9 VVDSKPFSLVDFFLSILRLMWLFFATLVSSEPVSQ-----HVQEYRSTTRIVPFGGGRSV 63 ++ S FSL FFL L L +LF +S V Q VQ S+++ G R V Sbjct: 401701 LLHSNTFSLRFFFLFFLFLFFLFIFAFNTSSKVRQ*LLRMRVQVSLSSSQSQKRGRNRQV 401522 Query: 64 G 64 G Sbjct: 401521 G 401519 Score = 24.6 bits (52), Expect = 9.9 Identities = 9/15 (60%), Positives = 9/15 (60%) Frame = +1 Query: 68 RGPNIHGLPKGSLTG 82 RGPN H P G L G Sbjct: 503263 RGPNAHAAPAGQLAG 503307 >supercontig_1.19 of Capsaspora owczarzaki ATCC 30864 Length = 161746 Score = 26.2 bits (56), Expect = 3.4 Identities = 11/18 (61%), Positives = 14/18 (77%) Frame = +1 Query: 18 VDFFLSILRLMWLFFATL 35 V FFLS+L L+WL F +L Sbjct: 112585 VSFFLSLLLLVWLCFLSL 112638 >supercontig_1.14 of Capsaspora owczarzaki ATCC 30864 Length = 919646 Score = 25.8 bits (55), Expect = 4.5 Identities = 8/17 (47%), Positives = 12/17 (70%) Frame = -3 Query: 16 SLVDFFLSILRLMWLFF 32 + +DFF +L+L W FF Sbjct: 219498 NFIDFFFFVLKLTWFFF 219448 >supercontig_1.10 of Capsaspora owczarzaki ATCC 30864 Length = 1357310 Score = 25.4 bits (54), Expect = 5.8 Identities = 13/25 (52%), Positives = 15/25 (60%) Frame = -2 Query: 8 RVVDSKPFSLVDFFLSILRLMWLFF 32 R V KPF FF +LRL+W FF Sbjct: 1189483 RRVGRKPFL---FFFLLLRLLWWFF 1189418 >supercontig_1.7 of Capsaspora owczarzaki ATCC 30864 Length = 1544042 Score = 25.0 bits (53), Expect = 7.6 Identities = 14/30 (46%), Positives = 17/30 (56%) Frame = -3 Query: 15 FSLVDFFLSILRLMWLFFATLVSSEPVSQH 44 F L FFL + L +LFF+ L S VS H Sbjct: 1355097 FPLFYFFLFLFFLFFLFFSFLFSCLVVSLH 1355008 Score = 25.0 bits (53), Expect = 7.6 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = +1 Query: 15 FSLVDFFLSILRLMWLFFATLVSSEPVSQHVQEYRSTTRIVPF 57 F + FFL L L + FF +L+ + H + RST + PF Sbjct: 1136188 FLFLLFFLLFLPLFFFFFVSLLL---LLLHCRRRRSTAKTPPF 1136307 Score = 24.6 bits (52), Expect = 9.9 Identities = 19/64 (29%), Positives = 25/64 (39%), Gaps = 8/64 (12%) Frame = -1 Query: 32 FATLVSSEPVSQHVQE--------YRSTTRIVPFGGGRSVGHAPRGPNIHGLPKGSLTGG 83 F +LVSS + +R T V G G S+ PN+ GS+T G Sbjct: 39749 FMSLVSSSRAPRRASSCAR*CSNIWRMRTSCVRIGDGNSM------PNVASNCSGSVTAG 39588 Query: 84 CATG 87 C G Sbjct: 39587 CEFG 39576 >supercontig_1.6 of Capsaspora owczarzaki ATCC 30864 Length = 1617845 Score = 25.0 bits (53), Expect = 7.6 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = +3 Query: 55 VPFGGGRSVGHAPRGPNIHGLPKG 78 +P G GRS GH+P P I +G Sbjct: 723876 LPHGPGRSQGHSPLWPCIRPARRG 723947 >supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 Length = 2668053 Score = 25.0 bits (53), Expect = 7.6 Identities = 17/41 (41%), Positives = 21/41 (51%) Frame = -2 Query: 20 FFLSILRLMWLFFATLVSSEPVSQHVQEYRSTTRIVPFGGG 60 FFL +L L+++F L SS V Y S V FGGG Sbjct: 2054810 FFLFVLFLLFVFVMRLRSSVDVLVLSLGYGSWMPGVAFGGG 2054688 >supercontig_1.11 of Capsaspora owczarzaki ATCC 30864 Length = 1292811 Score = 24.6 bits (52), Expect = 9.9 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 1/31 (3%) Frame = -1 Query: 55 VPFGG-GRSVGHAPRGPNIHGLPKGSLTGGC 84 VP G + V H P P+IH L KG + C Sbjct: 71409 VPTGRIAKQVSHLPVIPSIHSLQKGQRSQTC 71317 >supercontig_1.5 of Capsaspora owczarzaki ATCC 30864 Length = 1755934 Score = 24.6 bits (52), Expect = 9.9 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +1 Query: 59 GGRSVGHAPRGPNIHGLPKGSLTGGCAT 86 GG + P GP + G+P ++ GG +T Sbjct: 255121 GGGTTLIVPGGPGVLGVPNANVHGGYST 255204 Database: C.owczarzaki/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 28,043,798 Number of sequences in database: 89 Lambda K H 0.323 0.141 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,400,572 Number of Sequences: 89 Number of extensions: 49124 Number of successful extensions: 396 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 283 Number of HSP's gapped (non-prelim): 255 length of query: 89 length of database: 9,347,932 effective HSP length: 64 effective length of query: 25 effective length of database: 9,342,236 effective search space: 233555900 effective search space used: 233555900 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 52 (24.6 bits)