TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000112_1.0 # Protein # Selenoprotein K (SelK) # Mus musculus # Complete (94 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 3.9 emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 5.1 >emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_5 Length = 953317 Score = 25.4 bits (54), Expect = 3.9 Identities = 9/27 (33%), Positives = 14/27 (51%) Frame = -3 Query: 2 VYISNGQVLDSRNQSPWRVSFLTDFFW 28 +Y NG++L RN+ PW + W Sbjct: 722153 IYSRNGKLLAYRNREPWTLLNFEAIIW 722073 >emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_1 Length = 1866585 Score = 25.0 bits (53), Expect = 5.1 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = +2 Query: 3 YISNGQVLDSRNQSPWRVSFLTDFFWGIAEFVVFFF 38 YI + L S++ P V L F WG +VVF F Sbjct: 833489 YIQHEIELQSQSHHPCIVPLLECFRWGKRIYVVFSF 833596 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.332 0.142 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,103,417 Number of Sequences: 54 Number of extensions: 7791 Number of successful extensions: 61 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 59 Number of HSP's gapped (non-prelim): 8 length of query: 94 length of database: 6,272,415 effective HSP length: 69 effective length of query: 25 effective length of database: 6,268,689 effective search space: 156717225 effective search space used: 156717225 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 51 (24.3 bits)