TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000016_1.0 # Protein # Selenoprotein K (SelK) # Homo sapiens # Complete (94 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-t... 26 3.0 emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 5.1 emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 6.7 >emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_5 Length = 953317 Score = 25.8 bits (55), Expect = 3.0 Identities = 19/67 (28%), Positives = 27/67 (40%), Gaps = 8/67 (11%) Frame = -3 Query: 2 VYISNGQVLDSRSQSPWRLSLITDFFWGIAEFVVLFFKTLLQQD--------VKKRRSYG 53 +Y NG++L R++ PW L W I E F Q+ + K S Sbjct: 722153 IYSRNGKLLAYRNREPWTLLNFEAIIW-IQERKSCFICCQAQEKKEFSL*ILISKMSSSS 721977 Query: 54 NSSDSRY 60 NS +S Y Sbjct: 721976 NSFESEY 721956 >emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_0 Length = 1966360 Score = 25.0 bits (53), Expect = 5.1 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = +3 Query: 39 KTLLQQDVKKRRSYGNSSDSRYDD 62 +T+L DV+ R NSS+ RYDD Sbjct: 727020 ETVLCLDVEVWRQLENSSEPRYDD 727091 Score = 25.0 bits (53), Expect = 5.1 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = -3 Query: 39 KTLLQQDVKKRRSYGNSSDSRYDD 62 +T+L DV+ R NSS+ RYDD Sbjct: 827651 ETVLCLDVEVWRQLENSSEPRYDD 827580 Score = 24.6 bits (52), Expect = 6.7 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = +1 Query: 18 WRLSLITDFFWGIAEFVVLFFKTLLQQ 44 W ++ TD+ I+ + L FKTLLQ+ Sbjct: 136042 WLTTIETDWDRNISSVIKLSFKTLLQE 136122 >emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_4 Length = 1031885 Score = 24.6 bits (52), Expect = 6.7 Identities = 11/42 (26%), Positives = 20/42 (47%) Frame = +2 Query: 16 SPWRLSLITDFFWGIAEFVVLFFKTLLQQDVKKRRSYGNSSD 57 S + ++ G+A V + F + D K+ +YGN+ D Sbjct: 452966 SKMEVPIMVSSILGVASSVFIMFSAAARLDKKQLDNYGNNPD 453091 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.325 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,446,324 Number of Sequences: 54 Number of extensions: 14001 Number of successful extensions: 87 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 76 Number of HSP's gapped (non-prelim): 21 length of query: 94 length of database: 6,272,415 effective HSP length: 69 effective length of query: 25 effective length of database: 6,268,689 effective search space: 156717225 effective search space used: 156717225 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 51 (24.3 bits)