TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|70870239|gb|AAHK01001632.1|:subseq(1423,2260) Trypanosoma cruzi strain CL Brener tcruzi_1047053508727, whole genome shotgun sequence:[translate(1)] (89 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-t... 29 0.35 emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-t... 28 0.79 emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-t... 26 2.3 emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-t... 26 2.3 emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 3.9 emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 6.7 emb|FN668640.1| Blastocystis hominis, Singapore isolate B (sub-t... 24 8.7 emb|FN668642.1| Blastocystis hominis, Singapore isolate B (sub-t... 24 8.7 emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-t... 24 8.7 >emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_23 Length = 240939 Score = 28.9 bits (63), Expect = 0.35 Identities = 9/20 (45%), Positives = 16/20 (80%) Frame = +2 Query: 17 LVDFFLSILRLMWLFFATLV 36 L+ FF+S++ + W+FF TL+ Sbjct: 216953 LIIFFISVMNIFWMFFLTLM 217012 >emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_5 Length = 953317 Score = 27.7 bits (60), Expect = 0.79 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%) Frame = +2 Query: 61 RSVGHAPRGPNIHGLPKG--SLTGGCATGUR 89 R P GP +HGLP+G + GG G R Sbjct: 789695 RHAAAVPDGPRLHGLPRGVVEVHGGPGVGAR 789787 >emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_0 Length = 1966360 Score = 26.2 bits (56), Expect = 2.3 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = -2 Query: 47 EYRSTTRIVPFGGGRSVGHAPRGPNIHGLP 76 E R+ R P GRS+GH RG H +P Sbjct: 1738953 ERRARNRPRPPRSGRSLGHLDRGAADHPIP 1738864 Score = 24.3 bits (51), Expect = 8.7 Identities = 8/16 (50%), Positives = 11/16 (68%) Frame = -3 Query: 60 GRSVGHAPRGPNIHGL 75 G+S H P G +IHG+ Sbjct: 554906 GKSFAHVPNGNSIHGI 554859 Score = 24.3 bits (51), Expect = 8.7 Identities = 18/52 (34%), Positives = 25/52 (48%) Frame = -3 Query: 10 VDSKPFSLVDFFLSILRLMWLFFATLVSSEPVSQHVQEYRSTTRIVPFGGGR 61 +D +V FL L+L LFF LVS+ P+ Q + +R V G R Sbjct: 100925 IDQINEKMVSLFLGWLKLE-LFFPWLVSTRPLRTFDQ*ANTESRAVSHGRER 100773 >emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_2 Length = 1458350 Score = 26.2 bits (56), Expect = 2.3 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = +2 Query: 58 GGGRSVGHAPRGPNIHGLPKGSLTGGCATG 87 GGG G+A RGP H +G+ G C G Sbjct: 458858 GGG---GNAGRGPGNHEEARGNAAGDCVFG 458938 Score = 26.2 bits (56), Expect = 2.3 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = -2 Query: 58 GGGRSVGHAPRGPNIHGLPKGSLTGGCATG 87 GGG G+A RGP H +G+ G C G Sbjct: 727846 GGG---GNAGRGPGNHEEARGNAAGDCVFG 727766 Score = 25.4 bits (54), Expect = 3.9 Identities = 14/42 (33%), Positives = 25/42 (59%), Gaps = 3/42 (7%) Frame = +2 Query: 1 MPY---VANGRVVDSKPFSLVDFFLSILRLMWLFFATLVSSE 39 +PY + +VV S + L F +SIL+L ++FAT + ++ Sbjct: 723764 LPYAFQLTRNKVVKSIHYILYLF*MSILQLFQIYFATFIFNQ 723889 >emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_1 Length = 1866585 Score = 25.4 bits (54), Expect = 3.9 Identities = 9/12 (75%), Positives = 11/12 (91%) Frame = +2 Query: 72 IHGLPKGSLTGG 83 +HGLP+GSL GG Sbjct: 1073669 LHGLPRGSLCGG 1073704 Score = 25.4 bits (54), Expect = 3.9 Identities = 22/72 (30%), Positives = 29/72 (40%), Gaps = 13/72 (18%) Frame = -1 Query: 29 WLFFATLVSSEPVSQHVQEYRSTTRIVPFGG---GR--SVGHAPRGPNIH--------GL 75 WL +E V Q Q ++P G GR V H P GP +H GL Sbjct: 526578 WLHHGRAAEAEGVVQGEQLPAPNRAVLPLAGLPAGRRSDVYHEPEGPEVHQAAEVQGEGL 526399 Query: 76 PKGSLTGGCATG 87 +G + GG + G Sbjct: 526398 -QGGVPGGGSEG 526366 Score = 24.3 bits (51), Expect = 8.7 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -1 Query: 17 LVDFFLSILRLMWLFFATLVSSEPVS 42 +V S L LMW AT+V+ +P+S Sbjct: 923874 VVQLLHSQLILMWNILATIVTLQPIS 923797 >emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_3 Length = 1354787 Score = 24.6 bits (52), Expect = 6.7 Identities = 10/17 (58%), Positives = 10/17 (58%) Frame = -3 Query: 58 GGGRSVGHAPRGPNIHG 74 GGGR G A GP HG Sbjct: 1174446 GGGRHRGRAEEGPGDHG 1174396 Score = 24.6 bits (52), Expect = 6.7 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = +2 Query: 26 RLMWLFFATLVSSEPVSQHVQEYRSTT 52 RL +FA +V+ SQH+ Y S T Sbjct: 380633 RLGMFYFALIVAKSRASQHLDHYISPT 380713 >emb|FN668640.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_10 Length = 640322 Score = 24.3 bits (51), Expect = 8.7 Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 6/52 (11%) Frame = +1 Query: 8 RVVDSKPFSLVDFFLSILRLMWL------FFATLVSSEPVSQHVQEYRSTTR 53 RVV+ + L+ +F I+ +WL + TLV++ + + YRS+ + Sbjct: 307864 RVVNRRNIGLISYFHKIIMALWL*N*SI*YLTTLVTA--IGLLCRRYRSSRK 308013 >emb|FN668642.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_12 Length = 491035 Score = 24.3 bits (51), Expect = 8.7 Identities = 9/30 (30%), Positives = 14/30 (46%) Frame = +3 Query: 41 VSQHVQEYRSTTRIVPFGGGRSVGHAPRGP 70 V H++ RS R++ R +G GP Sbjct: 55632 VHHHIETVRSLIRVIQSSNEREIGFLQNGP 55721 >emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_9 Length = 642864 Score = 24.3 bits (51), Expect = 8.7 Identities = 13/42 (30%), Positives = 20/42 (47%) Frame = +1 Query: 16 SLVDFFLSILRLMWLFFATLVSSEPVSQHVQEYRSTTRIVPF 57 S + FLS L L+ L+ ++L S+ QH R + F Sbjct: 462010 SFLLLFLSFLHLLHLYLSSLPSTLTCQQHALSILQRRRFLQF 462135 Score = 24.3 bits (51), Expect = 8.7 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = +1 Query: 15 FSLVDFFLSILRLMWLFFATLVSSEP 40 FSL FFL + +W FF++ V P Sbjct: 468679 FSLSFFFLLLSCFLWFFFSSSVVVAP 468756 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.323 0.141 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,027,244 Number of Sequences: 54 Number of extensions: 28382 Number of successful extensions: 253 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 208 Number of HSP's gapped (non-prelim): 98 length of query: 89 length of database: 6,272,415 effective HSP length: 64 effective length of query: 25 effective length of database: 6,268,959 effective search space: 156723975 effective search space used: 156723975 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 51 (24.3 bits)