TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000047_1.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Drosophila melanogaster # Complete
         (398 letters)

Database: B.hominis/genome.fa 
           54 sequences; 18,817,245 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN668651.1| Blastocystis hominis, Singapore isolate B (sub-t...   287   7e-78
emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-t...    28   5.5  

>emb|FN668651.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome
              shotgun sequence assembly, scaffold_20
          Length = 315468

 Score =  287 bits (734), Expect = 7e-78
 Identities = 152/302 (50%), Positives = 196/302 (64%), Gaps = 12/302 (3%)
 Frame = +3

Query: 105    VQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLLAVSTKMTEKERDVVIP 164
              + TTDFFYP VDDPY+ GKIA  NVLSD+YAMGVT+ D MLM+L VS  M     D V  
Sbjct: 201756 ISTTDFFYPSVDDPYVQGKIAACNVLSDMYAMGVTEVDTMLMILGVSVDMEPDAMDYVTK 201935

Query: 165    LIMRGFK-----------DSALEAGTTVTGGQSVVNPWCTIGGVASTICQPNE-YIVPDN 212
               ++RGF            D A  A T VTGGQ+V NPW  IGGVAS+ C+  E +I P +
Sbjct: 201936 ELIRGFNGFPFLSSL*RVDQARVAVTNVTGGQTVRNPWPMIGGVASSTCREGEDFIRPVH 202115

Query: 213    AVVGDVLVLTKPLGTQVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMNSMARLNRV 272
                 GDV+VLTKPLGTQ+AVN  +W+    ++NR++ V+S EDV  +Y  AM SM+ LN  
Sbjct: 202116 GEPGDVIVLTKPLGTQLAVNMFEWLRTAAKFNRVRAVLSVEDVVASYKMAMESMSTLNLE 202295

Query: 273    AARLMHKYNAHGATDITGFGLLGHAQTLAAHQKKDVSFVIHNLPVIAKMAAVAKACGNMF 332
               ARLM K++AHG TD+TGFG+ GHA  LAA Q+K V  V+  LP+I  MA V     N +
Sbjct: 202296 GARLMWKHHAHGCTDVTGFGVTGHATNLAAEQEKAVDLVVDTLPIIKNMARVESHLNNNW 202475

Query: 333    QLLQGHSAETSGGLLICLPREQAAAYCKDIEKQEGYQAWIIGIVEKGNKTARIIDKPRVI 392
               L +G SAETSGGL+I +  E A AY K+ ++  G+ AWIIG V +G +   +++ P +I
Sbjct: 202476 NLFKGRSAETSGGLMIMMRPEDAEAYIKEYKEITGHDAWIIGKVVEGQRKGYVVENPTLI 202655

Query: 393    EV 394
              EV
Sbjct: 202656 EV 202661


>emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome
             shotgun sequence assembly, scaffold_9
          Length = 642864

 Score = 28.5 bits (62), Expect = 5.5
 Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
 Frame = +3

Query: 19    GDAELRRPFDPTAHDLDASFR--LTRFADLKGRGCKVPQDVL 58
             G+A+ RRPF P  HD+ + +   L R A  +    +VP+ VL
Sbjct: 72111 GNAQARRPFRPAGHDVPSRWHGSLLRPALRRDLPLRVPRQVL 72236


  Database: B.hominis/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 18,817,245
  Number of sequences in database:  54
  
Lambda     K      H
   0.321    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,064,786
Number of Sequences: 54
Number of extensions: 115998
Number of successful extensions: 517
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 509
Number of HSP's gapped (non-prelim): 7
length of query: 398
length of database: 6,272,415
effective HSP length: 99
effective length of query: 299
effective length of database: 6,267,069
effective search space: 1873853631
effective search space used: 1873853631
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)