TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Anopheles gambiae # Complete
         (369 letters)

Database: B.hominis/genome.fa 
           54 sequences; 18,817,245 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN668651.1| Blastocystis hominis, Singapore isolate B (sub-t...   237   5e-63
emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t...    31   1.0  
emb|FN668690.1| Blastocystis hominis, Singapore isolate B (sub-t...    29   3.8  
emb|FN668689.1| Blastocystis hominis, Singapore isolate B (sub-t...    28   6.5  

>emb|FN668651.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome
              shotgun sequence assembly, scaffold_20
          Length = 315468

 Score =  237 bits (605), Expect = 5e-63
 Identities = 127/312 (40%), Positives = 185/312 (59%), Gaps = 14/312 (4%)
 Frame = +3

Query: 66     SVIALKHDLFLVQSVDFFYPLIDDPFMLGKIALANVVSDVFAVGATEIDQIKLIVTAPTE 125
              +V+   H  + + + DFFYP +DDP++ GKIA  NV+SD++A+G TE+D + +I+    +
Sbjct: 201723 AVLHPSHR*YSISTTDFFYPSVDDPYVQGKIAACNVLSDMYAMGVTEVDTMLMILGVSVD 201902

Query: 126    FTEQEREVVVPMVMQGF-----------LEAAKACNAPVQIGSIAENPWCVIGGAASAVC 174
                    + V   +++GF           ++ A+     V  G    NPW +IGG AS+ C
Sbjct: 201903 MEPDAMDYVTKELIRGFNGFPFLSSL*RVDQARVAVTNVTGGQTVRNPWPMIGGVASSTC 202082

Query: 175    HRSE-LIMPYNAQPGDALVLTKPLGTQLATNAYIWMGEQQSDSWAQLRERFTVADIEQTY 233
                 E  I P + +PGD +VLTKPLGTQLA N + W+  + +  + ++R   +V D+  +Y
Sbjct: 202083 REGEDFIRPVHGEPGDVIVLTKPLGTQLAVNMFEWL--RTAAKFNRVRAVLSVEDVVASY 202256

Query: 234    RIALESMSRLNKTGAELMKKYGAHAATDVTGFGLYGHAENLASHQTADVDFHLDTLPIIK 293
              ++A+ESMS LN  GA LM K+ AH  TDVTGFG+ GHA NLA+ Q   VD  +DTLPIIK
Sbjct: 202257 KMAMESMSTLNLEGARLMWKHHAHGCTDVTGFGVTGHATNLAAEQEKAVDLVVDTLPIIK 202436

Query: 294    NVREIAETLGRGAKLLAGKAVETSGGLLICLPREAAAGFCEEYRRCTKHEAWIVGHVEKG 353
              N+  +   L     L  G++ ETSGGL+I +  E A  + +EY+  T H+AWI+G V +G
Sbjct: 202437 NMARVESHLNNNWNLFKGRSAETSGGLMIMMRPEDAEAYIKEYKEITGHDAWIIGKVVEG 202616

Query: 354    ARG--VKMNPNL 363
               R   V  NP L
Sbjct: 202617 QRKGYVVENPTL 202652


>emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun
               sequence assembly, scaffold_1
          Length = 1866585

 Score = 30.8 bits (68), Expect = 1.0
 Identities = 17/34 (50%), Positives = 22/34 (64%)
 Frame = +2

Query: 202     ATNAYIWMGEQQSDSWAQLRERFTVADIEQTYRI 235
               +TNA +   E + D  + L ERFTVADI QTY +
Sbjct: 1240748 STNAVL--EEVRMDECSGLLERFTVADISQTYHL 1240843


>emb|FN668690.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome
              shotgun sequence assembly, scaffold_8
          Length = 854764

 Score = 28.9 bits (63), Expect = 3.8
 Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 6/34 (17%)
 Frame = -2

Query: 37     LAGVYGEQLGDKDGKGPNKEEGV------GIGLD 64
              + G +G  +GD+DG G  + EGV      G+GLD
Sbjct: 480075 VGGRFGVGIGDEDGLGGERSEGVPIEGRFGVGLD 479974


>emb|FN668689.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome
              shotgun sequence assembly, scaffold_7
          Length = 870417

 Score = 28.1 bits (61), Expect = 6.5
 Identities = 11/35 (31%), Positives = 20/35 (57%)
 Frame = +3

Query: 145    AAKACNAPVQIGSIAENPWCVIGGAASAVCHRSEL 179
              ++ + N+P+   S     +C+I  ++SAVCH   L
Sbjct: 753813 SSDSVNSPIIFSSTLSTRYCIICPSSSAVCHSPSL 753917


  Database: B.hominis/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 18,817,245
  Number of sequences in database:  54
  
Lambda     K      H
   0.319    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,883,642
Number of Sequences: 54
Number of extensions: 91705
Number of successful extensions: 403
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 386
Number of HSP's gapped (non-prelim): 32
length of query: 369
length of database: 6,272,415
effective HSP length: 98
effective length of query: 271
effective length of database: 6,267,123
effective search space: 1698390333
effective search space used: 1698390333
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)