TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000037_1.0 # Protein # SECIS binding protein 2 (SBP2) # Homo sapiens # Complete (854 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 79 9e-15 gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 34 0.41 gb|CM000607.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 33 0.71 gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 30 7.8 >gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun sequence Length = 2535400 Score = 79.3 bits (194), Expect = 9e-15 Identities = 44/140 (31%), Positives = 75/140 (53%) Frame = +2 Query: 630 RDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVXXXXXXXXX 689 R Y + + + V +T++ EL+R Q R ++ K KRRLV+GLREV Sbjct: 1285895 RPYATLISDESVKPLLTEMFGELMRLQKRAVDEN--NTKVKRRLVMGLREVARGIRSHKV 1286068 Query: 690 XCVIISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGI 749 V ++ N + G +D+ L I+D A E +P + ++ALG+ + K V ++VVG+ Sbjct: 1286069 KLVAMANN---LDDYGAIDEKLQEILDLADENEVPIFYEFTKRALGKVMGKTVKIAVVGV 1286239 Query: 750 FSYDGAQDQFHKMVELTVAA 769 + +GA QF K++ ++ A Sbjct: 1286240 QNAEGAHQQFKKLLAISTKA 1286299 Score = 31.2 bits (69), Expect = 2.7 Identities = 32/130 (24%), Positives = 50/130 (38%) Frame = -2 Query: 527 KKPTSLKKIILKERQERKQRLQENAVSPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDE 586 + PT++ + L R QRLQ + + + GE D Q A L + Sbjct: 210954 RSPTAISRAQLSFADTRAQRLQSSVAATCNCLTLPETGEYRSDKQL---AIL*HAQPASF 210784 Query: 587 LISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFRDYCSQMLSKEVDACVT 646 + T + D +P GT D H + PK + + Y S E+ A +T Sbjct: 210783 MTDTAVIHD---DPSGTNNDDDAVTEHGDSGISHLPKKEAPDGKIYFSY---SEIHATIT 210622 Query: 647 DLLKELVRFQ 656 L+ +L FQ Sbjct: 210621 SLVPQLEAFQ 210592 Score = 30.8 bits (68), Expect = 3.5 Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 9/75 (12%) Frame = +1 Query: 509 DSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRL---------QENAVSPAFTSD 559 D + KG+QR P+ KK ++LKK +L+ER R + L + A S Sbjct: 1284691 DGKMVVKNKGRQRIRPQKKKFSALKKKVLEERLRRWRDLHPEDSLHSVERKASSLVCIIG 1284870 Query: 560 DTQDGESGGDDQFPE 574 T + E DD++ E Sbjct: 1284871 LTSEDELEDDDEYNE 1284915 >gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 8, whole genome shotgun sequence Length = 1007773 Score = 33.9 bits (76), Expect = 0.41 Identities = 36/139 (25%), Positives = 56/139 (40%), Gaps = 27/139 (19%) Frame = -2 Query: 511 SAPLMKKGKQREIPKAKKPTSLKKIILKE--RQERKQ----RLQENAVSPAFTSDDTQDG 564 SA L + GK KKPT LKK I K ++E K+ + ++ A + TS + Sbjct: 594090 SANLTEDGKLTNSSVWKKPTPLKKAIKKSIGKKELKKTVGTKRKKEASIDSKTSKKARKK 593911 Query: 565 ESGGDDQFPEQA---------------------ELSGPEGMDELISTPSVEDKSEEPPGT 603 +SG +++ P A +L G +G + S +V K P + Sbjct: 593910 KSGEEEKKPTTAPAKSKVQKVKVRVEKANPKVEQLQGDDGRSSVDSYSAVHQK-HGPQSS 593734 Query: 604 ELQRDTEASHLAPNHTTFP 622 Q+ H P+H FP Sbjct: 593733 VHQQSQPPPHWGPSHHNFP 593677 >gb|CM000607.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 4, whole genome shotgun sequence Length = 1360148 Score = 33.1 bits (74), Expect = 0.71 Identities = 19/56 (33%), Positives = 26/56 (46%), Gaps = 4/56 (7%) Frame = +3 Query: 759 FHKMVELTVAARQAYK----TMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPP 810 FHK+ + + R Y+ T+ E+V LVG P +PP P C GPP Sbjct: 170124 FHKVTYILTSYRYVYRPTRSTLFESVGTSLVGIP--SSPPFFPNTPTKCVGVAGPP 170285 >gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 21, whole genome shotgun sequence Length = 662217 Score = 29.6 bits (65), Expect = 7.8 Identities = 21/89 (23%), Positives = 33/89 (37%), Gaps = 6/89 (6%) Frame = -1 Query: 558 SDDTQDGESGGDDQFPEQAELSGPE------GMDELISTPSVEDKSEEPPGTELQRDTEA 611 SD TQ G A S E + S S + PP + + ++ + Sbjct: 589989 SDSTQSDHEEGHGTPLVSAPFSSSEMRFLTHALQATSSAVSAVTRRRPPPSHQARDESVS 589810 Query: 612 SHLAPNHTTFPKIHSRRFRDYCSQMLSKE 640 SH AP T P + S + C+ + S + Sbjct: 589809 SHAAPGVATLPILSSSSMAESCAALSSNQ 589723 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.311 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,746,388 Number of Sequences: 31 Number of extensions: 290347 Number of successful extensions: 1457 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 1334 Number of HSP's gapped (non-prelim): 302 length of query: 854 length of database: 7,911,228 effective HSP length: 107 effective length of query: 747 effective length of database: 7,907,911 effective search space: 5907209517 effective search space used: 5907209517 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 64 (29.3 bits)