TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000037_1.0 # Protein # SECIS binding protein 2 (SBP2) # Homo sapiens # Complete (854 letters) Database: N.gruberi/genome.fa 784 sequences; 40,964,085 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 105 1e-22 gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 37 0.083 gb|GG738868.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 35 0.24 gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 34 0.54 gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 33 0.92 gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 33 1.2 gb|GG738864.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 32 2.1 gb|GG738882.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 32 2.7 gb|GG738874.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 31 4.6 gb|GG738865.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 31 6.0 gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 31 6.0 gb|GG738925.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 30 7.8 gb|GG738871.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 30 7.8 gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 30 7.8 >gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_1, whole genome shotgun sequence Length = 1555553 Score = 105 bits (263), Expect = 1e-22 Identities = 74/252 (29%), Positives = 127/252 (50%), Gaps = 9/252 (3%) Frame = +2 Query: 523 IPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTSDDTQDGESGGDDQFPEQAELS-GP 581 + K KKP LKK+ILKE++ RK + ++P D + ++ +D E + P Sbjct: 898847 LTKKKKPNKLKKLILKEKEIRKL----HNIAPGEFLDVKKFLKNLKEDTETESVVIEIVP 899014 Query: 582 EGMDE---LISTPSVEDKSEEPPGTE--LQRDTEASHLA---PNHTTFPKIHSRRFRDYC 633 E +E L ++ ++ K ++ E L D E L+ + R+ R+Y Sbjct: 899015 EKTEETTILENSKTILTKEDDSSDGEEKLVADDEMDILSIAESMEVVLKNSNERKIREYV 899194 Query: 634 SQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVXXXXXXXXXXCVI 693 + L + + + +L +L +Q R+ + D K K + R V+G+REV V+ Sbjct: 899195 NYPLDPDYENIIFQMLTKLQFYQFRLKKTDKKKFKQRLRYVIGIREVLKGLKLKHIRAVV 899374 Query: 694 ISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYD 753 I+PN +K+ + GGLD + II EQ +P FAL +K LG ++ + +S+VG++S D Sbjct: 899375 IAPNIDKVTTPGGLDTKIEEIIQKCNEQELPVFFALTKKKLGTAMKTSTKISLVGLYSMD 899554 Query: 754 GAQDQFHKMVEL 765 GA D+F K ++ Sbjct: 899555 GAFDEFKKAKQI 899590 Score = 34.3 bits (77), Expect = 0.54 Identities = 26/103 (25%), Positives = 40/103 (38%) Frame = -3 Query: 268 GEIVVKNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKT 327 G + +NNP+ + +N N+P T + S M Y E S +N+ S +N Sbjct: 63903 GSSMHQNNPHSRIESNLYQNNPPSTNDDSINSMNYQNNPHSRNETSLY-QNIPSSMNQNY 63727 Query: 328 IASSADPKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGS 370 +S P NV ++ P+ E K K S S Sbjct: 63726 NSSQPQPSNVEQSKIPLYNTGPNLTDEFRTPTSQLKKKVSSSS 63598 >gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_4, whole genome shotgun sequence Length = 712659 Score = 37.0 bits (84), Expect = 0.083 Identities = 26/100 (26%), Positives = 48/100 (48%), Gaps = 2/100 (2%) Frame = -1 Query: 260 KPAAVLSKGEIVVKNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLSTEL-SAAPKN 318 KP + G+ VV +N + +++ + SPS T LS T ++ LST + P Sbjct: 474975 KPIPLYMSGDEVVNSNETTTASSSLTSPSPSVTSNLSTTTTTPMMMSNLSTTTPTTTPST 474796 Query: 319 VTSMINLKTIASSADPKNVSIPS-SEALSSDPSYNKEKHI 357 +++ +N +SS+ + I S A +++ Y+ HI Sbjct: 474795 LSTAVNHHQQSSSSTTTTIDIHDVSPANTNNQLYHLSNHI 474676 >gb|GG738868.1| Naegleria gruberi genomic scaffold NAEGRscaffold_24, whole genome shotgun sequence Length = 427589 Score = 35.4 bits (80), Expect = 0.24 Identities = 76/441 (17%), Positives = 156/441 (35%), Gaps = 33/441 (7%) Frame = -1 Query: 269 EIVVKNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTI 328 E +V ++TA + +R + TP V T +E S P N ++ K + Sbjct: 279455 EDIVPEGEMANLTAQLSELGILNSRIIDSTPPASPVPSTGVSEKSPVPSNPNHIVARKVV 279276 Query: 329 ASSADPKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGSDLEQNEAXXXXXXXXXXX 388 IPS P K + + P S+ S + + + + Sbjct: 279275 QPQTARNQTPIPSDLKSVPKPQILKVQPSVQPLPSSRPSSSNQVVKEKKIDQEILKLHKE 279096 Query: 389 XXXYEVLTVQ----EPPRIEDAEEFPNLAVASERRDRIETP-KFQSKQQPQDNFKNNV-- 441 E + Q + +++ E N + S++ + P KF+SKQ N K + Sbjct: 279095 QMRVEKMLEQVRREKEEKLKKEREENNFSEFSKKFQNLNVPSKFESKQNQFSNEKERILF 278916 Query: 442 KKSQLPVQLDLGGMLTALEKKQHSQHAKQXXXXXXXXXXXXXXXXXECASGERGRRMSQM 501 ++ Q Q + L++ + HA + + ++ +Q Sbjct: 278915 ERQQKRAQ-EEEQEKRKLQEARIESHANRLV--------------------AKNQQYNQF 278799 Query: 502 KTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQ-------------------- 541 +N L S +KG ++E+ + K PT ++ I ++ + Sbjct: 278798 HNQNNILAS-----EKGLKKEVDQVKSPTDIESITQRQAEIDSELEKEYTEMINLTHKKI 278634 Query: 542 ERKQRLQENAVS--PAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEE 599 E Q+ +E+ ++ P D QD G D +E E ++ + E++ + Sbjct: 278633 ETLQKKKESIMNLLPESEKDKQQDTMFGDSD----DSETVDSEDIEYETQSEDEENEYSD 278466 Query: 600 PPGTELQRDTEASHLAPNHTTFPKIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRM 659 E D + + + P + R D ++++++ D ++L + Sbjct: 278465 NEDEEENDDEDEYYDQTEYDDVPVV--GRLEDRVTKLITQNEQIFGKDRFQKLYAKYKDI 278292 Query: 660 YQKDPVKAK----TKRRLVLG 676 Y D ++ + T R+ +LG Sbjct: 278291 YNNDQIQNEEAKITPRKEILG 278229 >gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_62, whole genome shotgun sequence Length = 286411 Score = 34.3 bits (77), Expect = 0.54 Identities = 32/120 (26%), Positives = 55/120 (45%), Gaps = 4/120 (3%) Frame = +2 Query: 239 QPKWGPVHSVSTDISLLREVVKPAAVLSKGEIVVKNNPNESVTANAATNSPSCTRELSWT 298 QP V SVST L EV+KP+ K E + NP+ + + SP ++ S Sbjct: 254837 QPPPVVVASVSTQTFLAGEVIKPSTERQKSE--SERNPSPTTIRSV---SPVISQMRSSL 255001 Query: 299 PMGYVVRQTLSTELSAAPKNVTSMIN----LKTIASSADPKNVSIPSSEALSSDPSYNKE 354 V+++ + + +++ P++ S + LK P+++ E LSSD S K+ Sbjct: 255002 DKNEVIKKRIDSNVNSQPESRNSKRDSIDFLKYKMKLVKPEDIYSLFKEILSSDKSVRKQ 255181 >gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_14, whole genome shotgun sequence Length = 782252 Score = 33.5 bits (75), Expect = 0.92 Identities = 31/123 (25%), Positives = 54/123 (43%), Gaps = 8/123 (6%) Frame = -1 Query: 223 FPELQGAENNMSEIQKQPKWGPVHSVSTDISLLREVVKPAAVLSKGEIV--------VKN 274 FP + N+ E+QK+P + + R++ A +L K + + Sbjct: 694937 FPLNKNPSKNLEELQKRPPYNSQNL*KLFKFSRRKL*FIAFLLKKNLVF*PFNIITPL*K 694758 Query: 275 NPNESVTANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADP 334 NPN+++ N + S SC R LSW V+ L L N+ S +N T++++ P Sbjct: 694757 NPNKTIRNNTMS-STSCRRNLSWNVQQLVL---LLISLCMLIANILSDMNTITVSTNEYP 694590 Query: 335 KNV 337 +V Sbjct: 694589 SSV 694581 >gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_16, whole genome shotgun sequence Length = 536960 Score = 33.1 bits (74), Expect = 1.2 Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 2/118 (1%) Frame = +1 Query: 516 KKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTSDDTQDGESGGDDQFPEQ 575 K KQ++ PK+ KK K+ ++++++ +E A+S D DG+ G E+ Sbjct: 13954 KDDKQKDQPKSDNKEDKKKDEKKKNEKKQEQKKEVAISDDSWEDFDIDGD--GSAATTEK 14127 Query: 576 AELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLA--PNHTTFPKIHSRRFRD 631 E +E + E++ EE EL + + A N + ++ S+ R+ Sbjct: 14128 VETVKQPVKEEEVQVDDEEEEEEEKEEVELDDENTIGNAAEQSNEDSQTEVESQEDRE 14301 >gb|GG738864.1| Naegleria gruberi genomic scaffold NAEGRscaffold_20, whole genome shotgun sequence Length = 441990 Score = 32.3 bits (72), Expect = 2.1 Identities = 29/101 (28%), Positives = 48/101 (47%) Frame = +3 Query: 499 SQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTS 558 +Q KT N D +KK K+ K K+ K+ K+++ +K + Q N A Sbjct: 391647 AQAKTDDN--DELITKLKKEKESSDDKPKRQRKEKRE--KKKENKKDKKQVNNKRKA--- 391805 Query: 559 DDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEE 599 + DGES DD+ ++A+ S E D + +DK++E Sbjct: 391806 KEESDGES--DDEDKQEAKKSKYEAKDGELDEKDDDDKNDE 391922 >gb|GG738882.1| Naegleria gruberi genomic scaffold NAEGRscaffold_38, whole genome shotgun sequence Length = 381539 Score = 32.0 bits (71), Expect = 2.7 Identities = 20/82 (24%), Positives = 37/82 (45%) Frame = -3 Query: 273 KNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSA 332 KNN ++ V NSP T+ S TP+ + + + S++ N +++ + Sbjct: 113673 KNNKSQHVNEQPGINSPLITQNYSLTPLNLLQKSNPTLASSSSANNSIELVS--SSGQYL 113500 Query: 333 DPKNVSIPSSEALSSDPSYNKE 354 +P N S SS+PS + + Sbjct: 113499 EPSNPSSYLRPLKSSNPSSSND 113434 >gb|GG738874.1| Naegleria gruberi genomic scaffold NAEGRscaffold_30, whole genome shotgun sequence Length = 401612 Score = 31.2 bits (69), Expect = 4.6 Identities = 21/102 (20%), Positives = 39/102 (38%) Frame = +1 Query: 37 SSEACVFPSSAATYYPFVQEPPVTEQKIYTEDMAFGASTFPPQYLSSEITLHPYAYSPYT 96 S +A V P+ +Y V +P + +++ + T P Y + + PY Sbjct: 63394 SGQAFVQPAHPHVHYHHV-DPNMQRKQVIPPQVITQTGTQMPSYYYPQQPYYAVPGQPYG 63570 Query: 97 LDSTQNVYSVPGSQYLYNQPSCYRGFQTVKHRNENTCPLPQE 138 + Y+ G QY Y + Y + H+ P+P + Sbjct: 63571 MSPQSFPYAPYGYQYPYGAQTAYPPYPHQGHQPHTVPPIPNQ 63696 >gb|GG738865.1| Naegleria gruberi genomic scaffold NAEGRscaffold_21, whole genome shotgun sequence Length = 820293 Score = 30.8 bits (68), Expect = 6.0 Identities = 21/82 (25%), Positives = 43/82 (52%) Frame = +3 Query: 211 TSKPEFEFTTLDFPELQGAENNMSEIQKQPKWGPVHSVSTDISLLREVVKPAAVLSKGEI 270 +SKP+ + T + L NM++I ++P + P S++++ LRE+ P + + Sbjct: 36009 SSKPQLDITFNNQSAL-----NMTDISQKPLFTPTKP-SSNLAKLRELTSPNSPSTVFSS 36170 Query: 271 VVKNNPNESVTANAATNSPSCT 292 +V + ++ +T+ SPS T Sbjct: 36171 IVSKSLSKKLTSPPPKTSPSIT 36236 >gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_7, whole genome shotgun sequence Length = 693645 Score = 30.8 bits (68), Expect = 6.0 Identities = 44/177 (24%), Positives = 64/177 (36%), Gaps = 39/177 (22%) Frame = -1 Query: 161 SERADGTISSEIKSARGSHHLSIYAENSLKS--------------DGYH---KRTDRKSR 203 +E DGT SS + + LS A LK+ D ++ K T KS Sbjct: 631596 TETNDGTFSSVFSTPKSLKRLSNGATGLLKNKLDLFVSKGSTFADDDFYLGSKYTRSKSV 631417 Query: 204 IIAKNVSTSKPEFEF-------------TTLDFPELQGAENNMSEIQ---------KQPK 241 K+ S S PE E +T DF E NN + I+ K + Sbjct: 631416 SSLKSKSHSAPELESQAFDELIEEPTKSSTSDFDETTKIINNFNNIETLIDDMDGRKLVR 631237 Query: 242 WGPVHSVSTDISLLREVVKPAAVLSKGEIVVKNNPNESVTANAATNSPSCTRELSWT 298 + ++ L EVV+ ++ + +NP ES T SP T +S T Sbjct: 631236 EFMKKRCNEELLLYLEVVRTFKKINAAQCKQSSNPTESNTDGTPVESPRVTNLISAT 631066 Score = 30.4 bits (67), Expect = 7.8 Identities = 37/206 (17%), Positives = 78/206 (37%) Frame = -2 Query: 171 EIKSARGSHHLSIYAENSLKSDGYHKRTDRKSRIIAKNVSTSKPEFEFTTLDFPELQGAE 230 E+K + + E S + + + K ++ ++ E E T + P Q E Sbjct: 27755 EVKDPAAENQETKQVEKSGSENVVEEGKEAKENSVSSTSTSQIQEEEKTPSEDPPQQVGE 27576 Query: 231 NNMSEIQKQPKWGPVHSVSTDISLLREVVKPAAVLSKGEIVVKNNPNESVTANAATNSPS 290 N S + ++ +I+ +E +P E K+ E + + Sbjct: 27575 NTESNTPPVENVNTENVMTEEIATTKEETQPTV-----ENEEKSEVTEPIVEEVVEQVEN 27411 Query: 291 CTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPS 350 T+E + + ++T + + T+ N++ ++ DPK S+P S+ + +P+ Sbjct: 27410 NTKEQAEPEETSLPSTEITTTVEETTEPETTNENIQR--TTEDPKPGSVPLSQTVFDEPA 27237 Query: 351 YNKEKHIIHPTQKSKASQGSDLEQNE 376 + I Q + S +EQ E Sbjct: 27236 KKETNESITQEQTVEVS----IEQTE 27171 >gb|GG738925.1| Naegleria gruberi genomic scaffold NAEGRscaffold_81, whole genome shotgun sequence Length = 182562 Score = 30.4 bits (67), Expect = 7.8 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = -2 Query: 779 NVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEE 817 N +L+G+P+ PP T P+ DGP + K KEE Sbjct: 43904 NRSVQLLGQPKQTTPPKKVTPPPNNLDIDGPISKKRKEE 43788 >gb|GG738871.1| Naegleria gruberi genomic scaffold NAEGRscaffold_27, whole genome shotgun sequence Length = 417575 Score = 30.4 bits (67), Expect = 7.8 Identities = 15/54 (27%), Positives = 31/54 (57%) Frame = +3 Query: 273 KNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLK 326 +N+P S + ++TNS S +R+ W + Y Q +T ++ + +N++ N+K Sbjct: 155313 RNSPTCSTSTTSSTNSVSVSRDGFWKRIVY--NQCHTTNINTSTQNISCHYNIK 155468 >gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_10, whole genome shotgun sequence Length = 665159 Score = 30.4 bits (67), Expect = 7.8 Identities = 22/78 (28%), Positives = 29/78 (37%), Gaps = 5/78 (6%) Frame = -1 Query: 44 PSSAATYYPFVQE--PPVTEQKIYTEDMAFGASTFPPQYLSSEITLHPYA---YSPYTLD 98 P+ Y P +Q P T Q+ + +G PPQ + PY YSP T Sbjct: 113054 PNVVGQYSPQIQPFGQPQTNQQYPPQQGYYGQPNVPPQQYPPQYVNQPYGQPQYSPQT-- 112881 Query: 99 STQNVYSVPGSQYLYNQP 116 +PG Y QP Sbjct: 112880 ------GIPGQPQPYGQP 112845 Database: N.gruberi/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 40,964,085 Number of sequences in database: 784 Lambda K H 0.311 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 30,223,018 Number of Sequences: 784 Number of extensions: 374692 Number of successful extensions: 1586 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 40 Number of HSP's successfully gapped in prelim test: 57 Number of HSP's that attempted gapping in prelim test: 1251 Number of HSP's gapped (non-prelim): 756 length of query: 854 length of database: 13,654,695 effective HSP length: 111 effective length of query: 743 effective length of database: 13,567,671 effective search space: 10080779553 effective search space used: 10080779553 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 66 (30.0 bits)