TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000037_1.0 # Protein # SECIS binding protein 2 (SBP2) # Homo sapiens # Complete (854 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-t... 34 0.25 emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-t... 34 0.25 emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-t... 33 0.73 emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-t... 32 0.96 emb|FN668640.1| Blastocystis hominis, Singapore isolate B (sub-t... 32 1.3 emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t... 32 1.6 emb|FN668690.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 3.6 emb|FN668647.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 4.8 emb|FN668649.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 4.8 emb|FN668657.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 4.8 emb|FN668689.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 4.8 emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 6.2 emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 6.2 >emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_2 Length = 1458350 Score = 34.3 bits (77), Expect = 0.25 Identities = 46/233 (19%), Positives = 85/233 (36%), Gaps = 39/233 (16%) Frame = +1 Query: 423 ETPKFQSKQQPQDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQXXXXXXXXXXXX 482 +T K ++K++ K K +LP +L +E K +H + Sbjct: 1083961 QTVKEETKEESPKEEKKEEIKEELPKELPKEEPKPQIEAKPQ-RHGRDSIRRARKANKDA 1084137 Query: 483 XXXXXECASGERGRRMSQMKTPHNPLDSSAPLMKKGKQREIP------KAKKPTSL-KKI 535 E+ + + + P+ + P++++ K ++ KA++P + KK+ Sbjct: 1084138 AVAAPIQKENEKPKEPEKKEEKVEPIPAPVPVIEQEKVEKVEEKKPEEKAEEPLAEEKKV 1084317 Query: 536 ILKERQERKQRLQENAVSPAFTSDDTQDGESGGDDQFP---------------------- 573 L E K EN VSP ++ S G+D+ Sbjct: 1084318 ALSPIMEEK----ENEVSPVIEETKKEEMASSGEDEIVLSVSPSSAGKTPDTPSQSEDKK 1084485 Query: 574 -EQAELSGP---------EGMDELISTPSVEDKSEEPPGTELQRDTEASHLAP 616 E+A+ P E E + P VE+K EEP E +++ + + AP Sbjct: 1084486 LEEAKKEKPVVEEAKVEEEKKVESVPVPVVEEKKEEPEVEEEKKEEKPAESAP 1084644 >emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_4 Length = 1031885 Score = 34.3 bits (77), Expect = 0.25 Identities = 18/64 (28%), Positives = 33/64 (51%) Frame = -3 Query: 314 AAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGSDLE 373 ++ N TS K+ +PK + P S++P+ +KE + +P+ S S GSD Sbjct: 709134 SSESNETSSSPSKSSNEPNEPKEPNEPKESKESNEPNESKESNPSNPSNPSNPSNGSDAL 708955 Query: 374 QNEA 377 +N++ Sbjct: 708954 ENDS 708943 >emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_0 Length = 1966360 Score = 32.7 bits (73), Expect = 0.73 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = +1 Query: 788 PRPQAPPSLPTQGPSCPAEDGPPALKEKEEP 818 PRP PP+ P +GP P GPP + P Sbjct: 332425 PRPPRPPAAPPRGPPRPPPRGPPRAPPRAPP 332517 >emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_3 Length = 1354787 Score = 32.3 bits (72), Expect = 0.96 Identities = 25/110 (22%), Positives = 48/110 (43%) Frame = +2 Query: 334 PKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGSDLEQNEAXXXXXXXXXXXXXXYE 393 PK S S + SSD S ++E+ ++KSK S+ E +E Sbjct: 409604 PKKKSKKSKKESSSDESSSEEEKPKKKSKKSKKESSSESESSE----------------- 409732 Query: 394 VLTVQEPPRIEDAEEFPNLAVASERRDRIETPKFQSKQQPQDNFKNNVKK 443 +E P+ ++ + ++ SE + E PK + +++P++ K K+ Sbjct: 409733 ----EEKPKKKEKKAKKAVSSDSESSEEEEKPKEKKEEEPKEEPKEEAKE 409870 Score = 30.8 bits (68), Expect = 2.8 Identities = 17/45 (37%), Positives = 24/45 (53%) Frame = -3 Query: 730 NRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAYK 774 NR +SLN V +S IF+Y Q+ H + T A+RQ+ K Sbjct: 1262739 NRSTGSKSLNTYVQLSKCLIFAYT*TQNPTHPLQHYTAASRQSLK 1262605 >emb|FN668640.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_10 Length = 640322 Score = 32.0 bits (71), Expect = 1.3 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -3 Query: 790 PQAPPSLPTQGPSCPAEDGPPALKEK 815 P +PPS P PS P+ D PPA ++ Sbjct: 518934 PSSPPSFPPPAPSSPSPDPPPACVDR 518857 Score = 31.2 bits (69), Expect = 2.1 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 2/47 (4%) Frame = +1 Query: 542 ERKQRLQENAVSPAFTSDDTQD--GESGGDDQFPEQAELSGPEGMDE 586 ER + L+E PA DD ++ G G D+F E E G +G+DE Sbjct: 67456 ERDRLLEEQ--KPADVDDDAEE*SGREGSRDEFDEFDEFDGVDGVDE 67590 >emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_1 Length = 1866585 Score = 31.6 bits (70), Expect = 1.6 Identities = 30/108 (27%), Positives = 46/108 (42%), Gaps = 1/108 (0%) Frame = -1 Query: 564 GESG-GDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFP 622 G +G G Q +ELS DE+ P +D SEE + Q++ E S + P T Sbjct: 1020021 GRNGEGFSQLRRNSELSSD---DEISVIPFEDDMSEENNDEKRQKEKEDSSMTPAST--- 1019860 Query: 623 KIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTK 670 S+ FR+ + + K VD D L R+ + D + + K Sbjct: 1019859 --DSQNFRNLFGKKMKKIVDGKWRDCLDPKPRWVESTRVNDELVKEWK 1019722 >emb|FN668690.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_8 Length = 854764 Score = 30.4 bits (67), Expect = 3.6 Identities = 23/104 (22%), Positives = 42/104 (40%) Frame = +2 Query: 509 DSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTSDDTQDGESGG 568 DS KKGK AKK S + E++++ ++ +S D+ D +S Sbjct: 759104 DSDGKKKKKGKALAKKAAKKEESSSESSSDSESEKEEKKKKEEKKKEESSSDSSDSDSDS 759283 Query: 569 DDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEAS 612 D E+ E + +E E+ S + ++ D+ +S Sbjct: 759284 SDSESEKEEEKKKKEEEE---KKKKEESSSDSSDSDSDSDSSSS 759406 Score = 29.3 bits (64), Expect = 8.1 Identities = 17/47 (36%), Positives = 26/47 (55%) Frame = -2 Query: 254 LLREVVKPAAVLSKGEIVVKNNPNESVTANAATNSPSCTRELSWTPM 300 L+R V++P V SK +I + P SPSC+REL++ P+ Sbjct: 130983 LIRFVIRPIGVWSKKDIGEERIP---------LMSPSCSRELAFNPV 130870 >emb|FN668647.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_17 Length = 414243 Score = 30.0 bits (66), Expect = 4.8 Identities = 15/47 (31%), Positives = 26/47 (55%) Frame = -3 Query: 399 EPPRIEDAEEFPNLAVASERRDRIETPKFQSKQQPQDNFKNNVKKSQ 445 EP +ED EE P+LAV E+ ++ + + ++P N V+ +Q Sbjct: 322855 EPRALEDGEERPDLAVLGEKGKPVDELREDAAERPDVNRGGIVRAAQ 322715 >emb|FN668649.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_19 Length = 315857 Score = 30.0 bits (66), Expect = 4.8 Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = -3 Query: 707 LDDTLHTIIDYACE-QNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 756 L+ LH I CE +N+P+VF ++ ALGR+ PV I S + +Q Sbjct: 161625 LEIILH--IPLLCEDKNVPYVFVPSKIALGRACGVTRPVVACSIISDENSQ 161479 >emb|FN668657.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_26 Length = 145118 Score = 30.0 bits (66), Expect = 4.8 Identities = 32/158 (20%), Positives = 57/158 (36%), Gaps = 1/158 (0%) Frame = -3 Query: 454 GMLTALEKKQHSQHAKQXXXXXXXXXXXXXXXXXECASGERGRRMSQMKTPHNPLDSSAP 513 G++T+ S A Q + +GE + + + P Sbjct: 50121 GVMTSTSSLSLSSQASQTSAVLMDGKEEKMEEEEKRGNGETEQGKEENAEVNEPKVEEKE 49942 Query: 514 LMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVS-PAFTSDDTQDGESGGDDQF 572 + K E PK + + ++ I ++++E KQ QE P ++ Q+ + Sbjct: 49941 EKPEEKPEEKPKEESEENPEEKIEEKQEEEKQEKQEKPEEKPEEKIEEKQEEPEENHAEK 49762 Query: 573 PEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTE 610 PE+ E E S E+KSEE P + + E Sbjct: 49761 PEEKSEEKSEEKLEEKSEEKPEEKSEEKPEEKSEEKPE 49648 >emb|FN668689.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_7 Length = 870417 Score = 30.0 bits (66), Expect = 4.8 Identities = 18/71 (25%), Positives = 31/71 (43%) Frame = -1 Query: 536 ILKERQERKQRLQENAVSPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVED 595 +L++ +E +R + + T+ D G GGD + E P G+D + T Sbjct: 68871 VLRDAEEHGERYASHRAMDSPTAYDAGPGRHGGDGEVLVDEEGEEPAGLDHVDETADAAH 68692 Query: 596 KSEEPPGTELQ 606 +E G EL+ Sbjct: 68691 HAEH-EGVELE 68662 >emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_23 Length = 240939 Score = 29.6 bits (65), Expect = 6.2 Identities = 20/70 (28%), Positives = 28/70 (40%), Gaps = 10/70 (14%) Frame = -3 Query: 283 NAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKN----------VTSMINLKTIASSA 332 + A PSCT +SW + R S ++P TS N T +S++ Sbjct: 70027 SCAKQKPSCTNAISWFRFSFTTRCRSSRAFFSSPSTSRICPRWNHASTSFGNALTASSNS 69848 Query: 333 DPKNVSIPSS 342 VS PSS Sbjct: 69847 SCARVSSPSS 69818 >emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_5 Length = 953317 Score = 29.6 bits (65), Expect = 6.2 Identities = 12/22 (54%), Positives = 17/22 (77%) Frame = +2 Query: 160 DSERADGTISSEIKSARGSHHL 181 D++RADG +S ++SARG H L Sbjct: 561803 DADRADGAMSECVQSARGDHSL 561868 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.311 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,767,745 Number of Sequences: 54 Number of extensions: 220506 Number of successful extensions: 1478 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 1228 Number of HSP's gapped (non-prelim): 803 length of query: 854 length of database: 6,272,415 effective HSP length: 105 effective length of query: 749 effective length of database: 6,266,745 effective search space: 4693792005 effective search space used: 4693792005 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 63 (28.9 bits)