TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|116242735|sp|Q8IV42.2|PSTK_HUMAN RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase (348 letters) Database: S.parasitica/genome.fa 1445 sequences; 53,132,636 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG743912.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 51 2e-06 gb|GG743888.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 38 0.021 gb|GG743885.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 33 0.39 gb|GG743900.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 32 0.87 gb|GG743919.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 31 1.9 gb|GG743887.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 31 1.9 gb|GG744279.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 30 5.6 gb|GG744323.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 29 7.4 gb|GG743893.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 29 7.4 gb|GG743962.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 29 9.6 gb|GG743892.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 29 9.6 >gb|GG743912.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.30, whole genome shotgun sequence Length = 383214 Score = 51.2 bits (121), Expect = 2e-06 Identities = 54/212 (25%), Positives = 81/212 (38%), Gaps = 5/212 (2%) Frame = -2 Query: 20 LCVLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMPDAFLAGARARPAPSQWKLL 79 L +LCGLP AGKSTFARA+A + + + +++DDV F A + AP QWK Sbjct: 270758 LLLLCGLPGAGKSTFARAIADAASRT--YEVRCISFDDVFAAHFAADGGSF-APQQWKAA 270588 Query: 80 RQELLKYLEYFLMAVINGCQMS----VPPNRTEAMWEDFITCLKDQDLIFSAAFEA-QSC 134 + +E L A + + R + T L Q ++ A + Q C Sbjct: 270587 VAAIYTEVESALAAASTSGKTHM*AILVSLRCRYQLQLL*TALG*QHVLPQHAQKVLQLC 270408 Query: 135 YLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCPLETCLQRNGQRP 194 T + V+ N GF + +D + C RN R Sbjct: 270407 RQRTTHRADKDGESVVLGN------------------AGFGVIHVDTKPDLCRARNAARQ 270282 Query: 195 QALPPETIHLMGRKLEKPNPEKNAWEHNSLTI 226 +P M E P+P + WE S+ + Sbjct: 270281 SPVPDVVFRRMHALFEPPSPSMHPWEARSIVV 270186 >gb|GG743888.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.6, whole genome shotgun sequence Length = 961754 Score = 37.7 bits (86), Expect = 0.021 Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 1/60 (1%) Frame = +1 Query: 142 VSRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCPLETCLQRNGQRP-QALPPE 200 +S ++LD Y + RYE++ LA++ S C +++D P+ RN +R PPE Sbjct: 355789 LSADKIVILDTLNYIKGCRYELFCLAKELSTTHCVVYIDTPVAMAAARNAERAGDNFPPE 355968 Score = 30.4 bits (67), Expect = 3.3 Identities = 18/67 (26%), Positives = 29/67 (43%), Gaps = 1/67 (1%) Frame = +2 Query: 273 LHKTDQTLRRIVSQTMKEAKGNQEAFSEMTFKQRWVR-ANHAAIWRIILGNEHIKCRSAK 331 LH+ + +SQT K+ K AF + +WV NH W ++ ++ K + Sbjct: 45737 LHEAQDVYFQALSQTSKQLKIESSAFELKVLETQWVSCVNHLCQWPLL--HDFAKATQNQ 45910 Query: 332 VGWLQCC 338 L CC Sbjct: 45911 ELLLDCC 45931 >gb|GG743885.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.3, whole genome shotgun sequence Length = 1275006 Score = 33.5 bits (75), Expect = 0.39 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = -2 Query: 317 RIILGNEHIKCRSAKVGWLQCCRIEKRPLSTG 348 R++ N +CR+A+V W CCR RP TG Sbjct: 345980 RLVPRNRACRCRAARVLWP*CCRAPTRPWRTG 345885 >gb|GG743900.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.18, whole genome shotgun sequence Length = 504189 Score = 32.3 bits (72), Expect = 0.87 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +2 Query: 194 PQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPA 230 P PP + H R+L +P+P ++ H T PSP+ Sbjct: 474461 PSRAPPTSAHRPRRRLRRPSPRRSTRLHRPATHPSPS 474571 >gb|GG743919.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.37, whole genome shotgun sequence Length = 335642 Score = 31.2 bits (69), Expect = 1.9 Identities = 42/178 (23%), Positives = 66/178 (37%), Gaps = 11/178 (6%) Frame = -2 Query: 182 PLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEH--NSLTIPSPACASEASLEV 239 P+ TC NG RP+ + + P+ K+AW +SL PSP AS Sbjct: 222982 PMSTCHTPNGSRPKI----GLKMPSAPTRSPSDRKSAWRSSTSSLRRPSPRRASRTHRTT 222815 Query: 240 TDLLLTALENPVKYAEDNMEQKDTDRII---CSTNILHK-TDQTLRRIVSQTMKEAKGNQ 295 T T K M + T R++ C T K T +T + ++T + + + Sbjct: 222814 TGASATRCWARSKRPRPRM*RPTTKRLLRRPCHTKSTAKSTMRTASSLRTRTSRSRRCSP 222635 Query: 296 EAFS--EMTFKQRW-VRANHAAIWRIILGN--EHIKCRSAKVGWLQCCRIEKRPLSTG 348 S F W R+ H A + L + + S + W C R ++G Sbjct: 222634 RPSSTRRAFFSTSWGRRSRHRASPSLALRSLWRSTRSSSRRSNWRASCTSGFRRCASG 222461 >gb|GG743887.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.5, whole genome shotgun sequence Length = 1129684 Score = 31.2 bits (69), Expect = 1.9 Identities = 23/74 (31%), Positives = 39/74 (52%), Gaps = 3/74 (4%) Frame = -2 Query: 120 DQDLIFSAAFEAQSCYLLTKTAV---SRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQ 176 ++DL FSAA A+S + + A + ++ Y++ R EV L K +L F + Sbjct: 1012914 NRDLGFSAADRAESVRRVGEVAALFAAAGTVTIVGLVSPYRAHRDEVRALHEKMNLPFYE 1012735 Query: 177 LFLDCPLETCLQRN 190 +F+D PLE + R+ Sbjct: 1012734 VFVDTPLEVVMARD 1012693 >gb|GG744279.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.397, whole genome shotgun sequence Length = 12835 Score = 29.6 bits (65), Expect = 5.6 Identities = 25/65 (38%), Positives = 29/65 (44%) Frame = -1 Query: 7 IRGTGSDGPRKRGLCVLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMPDAFLAG 66 +RG + R RGL GL AA AR + HR Q G A +DV D LA Sbjct: 9070 LRGLCRNLTRPRGLRRRPGLVAARTKATARLITHRCSQRTGTRSRHAASNDV--DRPLAT 8897 Query: 67 ARARP 71 RA P Sbjct: 8896 DRASP 8882 >gb|GG744323.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.441, whole genome shotgun sequence Length = 9840 Score = 29.3 bits (64), Expect = 7.4 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 2/54 (3%) Frame = -2 Query: 182 PLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEH--NSLTIPSPACAS 233 P+ TC NG RP+ + + P+ K+AW +SL PSP AS Sbjct: 9221 PMSTCHMPNGSRPKI----GLKMPSAPTRSPSDRKSAWRSSTSSLRRPSPRRAS 9072 >gb|GG743893.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.11, whole genome shotgun sequence Length = 752600 Score = 29.3 bits (64), Expect = 7.4 Identities = 18/86 (20%), Positives = 39/86 (45%), Gaps = 5/86 (5%) Frame = +2 Query: 267 ICSTNILHKTDQTLRRIVSQTMKEAKGNQEAFSEMTFKQRWV-RANHAAIWRIILGNEHI 325 + + ++L D+ +RR+ T +A + Q W+ + + I+ + I Sbjct: 603938 LAAASVLDNVDRIIRRLHDDTALLVVKPLDAVNLSPVLQDWMTKMDFGHTQSIVRSRQDI 604117 Query: 326 K----CRSAKVGWLQCCRIEKRPLST 347 + CR+ + + CC++ +R LST Sbjct: 604118 RLRPACRATRCALVGCCKVYRRQLST 604195 >gb|GG743962.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.80, whole genome shotgun sequence Length = 147863 Score = 28.9 bits (63), Expect = 9.6 Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 1/36 (2%) Frame = -3 Query: 178 FLDCPLETCLQRN-GQRPQALPPETIHLMGRKLEKP 212 F CP++ CL+R+ RPQA P + + LM R +P Sbjct: 55605 FRPCPIQPCLRRDRSARPQA-PRQGLSLMARTSARP 55501 >gb|GG743892.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.10, whole genome shotgun sequence Length = 790954 Score = 28.9 bits (63), Expect = 9.6 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = +1 Query: 74 SQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRTEA 109 S WKLL Q L+Y+ +F ++ I+ Q++ T A Sbjct: 321964 SMWKLLPQPCLQYVRFFSLSTISSWQIAHTSPSTSA 322071 Database: S.parasitica/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 53,132,636 Number of sequences in database: 1445 Lambda K H 0.321 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 28,611,555 Number of Sequences: 1445 Number of extensions: 519355 Number of successful extensions: 3353 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 211 Number of HSP's successfully gapped in prelim test: 77 Number of HSP's that attempted gapping in prelim test: 2093 Number of HSP's gapped (non-prelim): 2055 length of query: 348 length of database: 17,710,878 effective HSP length: 105 effective length of query: 243 effective length of database: 17,559,153 effective search space: 4266874179 effective search space used: 4266874179 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 63 (28.9 bits)