TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|116242735|sp|Q8IV42.2|PSTK_HUMAN RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase (348 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000632.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 54 2e-07 gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 31 0.89 gb|CM000610.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 30 1.5 gb|CM000634.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 30 2.6 gb|CM000618.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 29 3.4 gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 29 3.4 gb|CM000628.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 5.8 gb|CM000631.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 9.9 gb|CM000626.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 9.9 gb|CM000614.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 9.9 gb|CM000613.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 9.9 >gb|CM000632.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 30, whole genome shotgun sequence Length = 317207 Score = 53.5 bits (127), Expect = 2e-07 Identities = 68/280 (24%), Positives = 108/280 (38%), Gaps = 17/280 (6%) Frame = -3 Query: 22 VLCGLPAAGKSTFARALAHRLQQEQGWAIGV--VAYDDVMPDAFLAGARARPAPSQWKLL 79 ++CGLP GKST AR+L + I V V YD + LA W+ Sbjct: 118230 LVCGLPGCGKSTLARSLCSSCTDDPHSPIHVDYVEYDALQET--LARRDNLTPLEAWRRT 118057 Query: 80 RQELLKYLEYFLM-AVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQSCYLLT 138 R L+++ FL AV G +T + Q Sbjct: 118056 RTVALEHVRSFLTHAVTTG---------------PIVTNTQPQQ---------------- 117970 Query: 139 KTAVSRPLFLVLDDNFYYQSMRYEVYQLARKY-----SLGFCQLFLDCPLETCLQRNGQR 193 ++P L+LDDNF+ + MR +VY++ R + ++ F +L P + L RN R Sbjct: 117969 ---PAQPHVLLLDDNFHLRGMRQQVYRVCRSFVTPTVAVAFGIAWLAVPSDRALSRNAGR 117799 Query: 194 --PQALPPETIHLMGRKLEKPNPEKNAWEHN-------SLTIPSPACASEASLEVTDLLL 244 P+ + T + E P+P + +WE + T P ++ + D + Sbjct: 117798 DPPRRVTTVTWDRLAAAAEPPDPTR-SWETHVRVFRLGDHTTDDPHASTRHVSVLRDWIA 117622 Query: 245 TALENPVKYAEDNMEQKDTDRIICSTNILHKTDQTLRRIV 284 +A + +TD + N H DQ LRR+V Sbjct: 117621 SAAPRNDETVPWLPPVVETDAVPVPANEAHVRDQQLRRLV 117502 >gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 6, whole genome shotgun sequence Length = 1035082 Score = 31.2 bits (69), Expect = 0.89 Identities = 18/43 (41%), Positives = 24/43 (55%) Frame = +3 Query: 20 LCVLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMPDA 62 + VL G GKST A L+H L QG+A+G++ D P A Sbjct: 877827 ILVLSGKGGVGKSTVAAQLSHTL-SNQGYAVGLLDVDLCGPSA 877952 >gb|CM000610.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 7, whole genome shotgun sequence Length = 1029019 Score = 30.4 bits (67), Expect = 1.5 Identities = 30/120 (25%), Positives = 46/120 (38%), Gaps = 5/120 (4%) Frame = +3 Query: 121 QDLIFSAAFEAQSCYLLTKTAVSRP-----LFLVLDDNFYYQSMRYEVYQLARKYSLGFC 175 QDL S FEA + + L T RP F D + + Y+V Sbjct: 55911 QDLFESRKFEANAAFRLETTGQRRPRRRWDFFNGFDVFAEREIVPYQVGGGNAAAHHIVR 56090 Query: 176 QLFLDCPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPACASEA 235 Q+ + +R G+RP + + GR+ E PN K W + + +P + EA Sbjct: 56091 QVLRIAVSQVLAERGGRRPVYACGKVVSHHGRRHESPNSRKPGWWVDDQDLTNPDGSPEA 56270 >gb|CM000634.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 32, whole genome shotgun sequence Length = 157053 Score = 29.6 bits (65), Expect = 2.6 Identities = 31/99 (31%), Positives = 39/99 (39%), Gaps = 5/99 (5%) Frame = -2 Query: 136 LLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFL-----DCPLETCLQRN 190 LL SRPL L +QS R + Y L L F Q L PL QR Sbjct: 56624 LLRVVQSSRPLLRRLYLPHLHQSSRPQAYPLRLLEVLNFPQPHLLRREAPLPLREASQR- 56448 Query: 191 GQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSP 229 R +A PP + L+ + L + P SL P+P Sbjct: 56447 --RREAFPPLRLRLVSQLLREMYPLLVLTSPKSLVCPTP 56337 >gb|CM000618.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 16, whole genome shotgun sequence Length = 764225 Score = 29.3 bits (64), Expect = 3.4 Identities = 14/53 (26%), Positives = 24/53 (45%) Frame = -3 Query: 211 KPNPEKNAWEHNSLTIPSPACASEASLEVTDLLLTALENPVKYAEDNMEQKDT 263 +P P ++W+ IP P A S L L+ +EN ++ N + D+ Sbjct: 637803 EPIPPVSSWKSKDTDIPEPIDAESVSKTSQGLFLSPIENAKRHPPFNNSEDDS 637645 >gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun sequence Length = 2535400 Score = 29.3 bits (64), Expect = 3.4 Identities = 18/49 (36%), Positives = 26/49 (53%) Frame = +1 Query: 22 VLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMPDAFLAGARAR 70 +L G GKS A A+A+ L Q WAI V+ +++ LA +AR Sbjct: 2278342 LLTGCAGVGKSWLASAVANTLTVSQSWAIHWVSCQELL----LAAGQAR 2278476 Score = 28.9 bits (63), Expect = 4.4 Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 3/58 (5%) Frame = +2 Query: 106 RTEAMW---EDFITCLKDQDLIFSAAFEAQSCYLLTKTAVSRPLFLVLDDNFYYQSMR 160 +T MW E + +CL + +A F + C +TKT +S + DNFY+Q+MR Sbjct: 1568978 KTILMWSARELWHSCLPTE---VNAKFHPKRCLWITKT-ISTLMTSADGDNFYFQTMR 1569139 >gb|CM000628.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 26, whole genome shotgun sequence Length = 441226 Score = 28.5 bits (62), Expect = 5.8 Identities = 12/43 (27%), Positives = 22/43 (51%) Frame = -1 Query: 301 MTFKQRWVRANHAAIWRIILGNEHIKCRSAKVGWLQCCRIEKR 343 M K RW++ + A I + +G + + + GW +C + KR Sbjct: 279913 MRAKPRWLQTSRATIS*LAVGRDSVSR*KLEHGWKKCTKTTKR 279785 >gb|CM000631.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 29, whole genome shotgun sequence Length = 384256 Score = 27.7 bits (60), Expect = 9.9 Identities = 15/30 (50%), Positives = 18/30 (60%) Frame = +3 Query: 91 LMAVINGCQMSVPPNRTEAMWEDFITCLKD 120 L VIN Q +V P+ T+ WED T LKD Sbjct: 11085 LRDVINSVQ-NVYPSETDMSWEDLRTVLKD 11171 >gb|CM000626.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 24, whole genome shotgun sequence Length = 511739 Score = 27.7 bits (60), Expect = 9.9 Identities = 15/30 (50%), Positives = 18/30 (60%) Frame = +1 Query: 91 LMAVINGCQMSVPPNRTEAMWEDFITCLKD 120 L VIN Q +V P+ T+ WED T LKD Sbjct: 493408 LRDVINSVQ-NVYPSETDMSWEDLRTVLKD 493494 >gb|CM000614.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 12, whole genome shotgun sequence Length = 901853 Score = 27.7 bits (60), Expect = 9.9 Identities = 20/61 (32%), Positives = 27/61 (44%), Gaps = 3/61 (4%) Frame = +3 Query: 179 LDCPLETCLQRN---GQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPACASEA 235 LD L+ C N G + LPP HL+ R+ + N WE TI P+ S A Sbjct: 889002 LDPDLQECSGGNRVEGVEEEGLPP---HLLFRRPLHQSVPVNPWEFPDSTIRKPSALSSA 889172 Query: 236 S 236 + Sbjct: 889173 A 889175 >gb|CM000613.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 10, whole genome shotgun sequence Length = 976485 Score = 27.7 bits (60), Expect = 9.9 Identities = 14/56 (25%), Positives = 23/56 (41%) Frame = +3 Query: 211 KPNPEKNAWEHNSLTIPSPACASEASLEVTDLLLTALENPVKYAEDNMEQKDTDRI 266 +P P+ H S P P S +E+ D T E + + D +E D + + Sbjct: 224367 QPQPQTPTDTHQSAHPPRPPFRSFPKMEMFDCCFTCFERGYESSGDEIEDDDAENL 224534 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.321 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,586,944 Number of Sequences: 31 Number of extensions: 194092 Number of successful extensions: 1097 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 1011 Number of HSP's gapped (non-prelim): 182 length of query: 348 length of database: 7,911,228 effective HSP length: 99 effective length of query: 249 effective length of database: 7,908,159 effective search space: 1969131591 effective search space used: 1969131591 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 60 (27.7 bits)