TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|116242735|sp|Q8IV42.2|PSTK_HUMAN RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase (348 letters) Database: P.pallidum/genome.fa 42 sequences; 32,942,533 total letters Searching..........................................done Score E Sequences producing significant alignments: (bits) Value gb|GL290985.1| Polysphondylium pallidum PN500 unplaced genomic s... 36 1e-06 gb|GL290983.1| Polysphondylium pallidum PN500 unplaced genomic s... 31 1.6 gb|GL290991.1| Polysphondylium pallidum PN500 unplaced genomic s... 30 3.6 gb|GL290993.1| Polysphondylium pallidum PN500 unplaced genomic s... 29 4.7 gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic s... 28 8.0 >gb|GL290985.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold3, whole genome shotgun sequence Length = 2290287 Score = 36.2 bits (82), Expect(2) = 1e-06 Identities = 14/25 (56%), Positives = 21/25 (84%) Frame = +2 Query: 148 LVLDDNFYYQSMRYEVYQLARKYSL 172 ++LDDNFYY+SMRY Y++A++ L Sbjct: 850751 ILLDDNFYYKSMRYIYYKIAKQCKL 850825 Score = 33.9 bits (76), Expect(2) = 1e-06 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 13/100 (13%) Frame = +3 Query: 169 KYSLGFCQLFLDCPLETCLQRNGQRPQAL-------------PPETIHLMGRKLEKPNPE 215 K + GF QLFL L N R Q L + I M + E PNP+ Sbjct: 850905 KDNTGFTQLFLYKDLYLSKLNNKLRLQQLLNCQTKPFEQLIVTDDVIDKMNQIFEDPNPQ 851084 Query: 216 KNAWEHNSLTIPSPACASEASLEVTDLLLTALENPVKYAE 255 + WE N++ IP+ ++ S LL T L+ + + E Sbjct: 851085 SHKWELNTVVIPTINSSNHNSSVSM*LLFTYLKLSLIFIE 851204 Score = 30.0 bits (66), Expect = 2.7 Identities = 15/42 (35%), Positives = 24/42 (57%) Frame = +2 Query: 298 FSEMTFKQRWVRANHAAIWRIILGNEHIKCRSAKVGWLQCCR 339 FS + +QR+ + H+ WR ++ + + KCR VG CCR Sbjct: 1805981 FSGRSSRQRFRASVHSISWRRVV-SAYCKCRLFSVGISGCCR 1806103 >gb|GL290983.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold1, whole genome shotgun sequence Length = 1995969 Score = 30.8 bits (68), Expect = 1.6 Identities = 43/188 (22%), Positives = 74/188 (39%), Gaps = 14/188 (7%) Frame = -3 Query: 129 FEAQSCYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCPLETCLQ 188 F+ YL+ T S LV FYYQ + E Y + K LG + D + Sbjct: 128737 FQTFKRYLIAATVGST---LVGGAYFYYQYLGNENYMFSSK--LGVSKDTFDKYNQLVEN 128573 Query: 189 RNGQRPQALPPET-----IHLMGRKLEKPNPEKNAWEHNSLT-------IPSPACASEAS 236 Q PQ I + +L +PN + E ++T I S A+ Sbjct: 128572 LKEQSPQTATATEADSTQIKRLQDQLVQPNETDTSVESVAVTSLPTKSEIKSIVQQQNAN 128393 Query: 237 LEVTDLLLTALENPVKYAEDNMEQKDTDRIICSTN--ILHKTDQTLRRIVSQTMKEAKGN 294 E+ ++ LTA P+ EDN DT ++ S N ++ +++ + ++ K+ + Sbjct: 128392 EELPEIRLTASGAPINQEEDN----DTTSVVVSLNNDVVSQSNTDSDSVTTEQSKQQQAR 128225 Query: 295 QEAFSEMT 302 + +T Sbjct: 128224 ADIIENLT 128201 >gb|GL290991.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold9, whole genome shotgun sequence Length = 2264991 Score = 29.6 bits (65), Expect = 3.6 Identities = 20/86 (23%), Positives = 41/86 (47%), Gaps = 3/86 (3%) Frame = -3 Query: 227 PSPACASEASLEVTDLLLTALENPVKYAEDNMEQKD---TDRIICSTNILHKTDQTLRRI 283 P+P+ +S +S L+ L++ +K +D ++QKD + I + + +K Q R Sbjct: 1696501 PAPSSSSSSSSSKDTKLIADLQDQLKQKDDQLKQKDDHLRQKEIANQELTNKNKQLEREK 1696322 Query: 284 VSQTMKEAKGNQEAFSEMTFKQRWVR 309 S T + N + E+ +R ++ Sbjct: 1696321 NSPTTASPQLNGHSSEEVESLKREIQ 1696244 >gb|GL290993.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold11, whole genome shotgun sequence Length = 1505626 Score = 29.3 bits (64), Expect = 4.7 Identities = 17/44 (38%), Positives = 25/44 (56%) Frame = +3 Query: 2 KTAENIRGTGSDGPRKRGLCVLCGLPAAGKSTFARALAHRLQQE 45 K+A+ G + PR +L G ++GK T ARALAH L ++ Sbjct: 72912 KSAKQYEKYGLEKPRS---LLLYGPASSGKRTLARALAHELGEQ 73034 >gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold8, whole genome shotgun sequence Length = 2270872 Score = 28.5 bits (62), Expect = 8.0 Identities = 11/33 (33%), Positives = 22/33 (66%) Frame = -3 Query: 233 SEASLEVTDLLLTALENPVKYAEDNMEQKDTDR 265 S+ + E+ + LL +NP Y+E++ +QK+ D+ Sbjct: 2233247 SDPTFELYNKLLKHFDNPTGYSEEDEQQKEQDK 2233149 Database: P.pallidum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 32,942,533 Number of sequences in database: 42 Lambda K H 0.321 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,227,988 Number of Sequences: 42 Number of extensions: 195648 Number of successful extensions: 815 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 764 Number of HSP's gapped (non-prelim): 192 length of query: 348 length of database: 10,980,844 effective HSP length: 101 effective length of query: 247 effective length of database: 10,976,602 effective search space: 2711220694 effective search space used: 2711220694 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 61 (28.1 bits)