TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|116242735|sp|Q8IV42.2|PSTK_HUMAN RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase (348 letters) Database: H.arabidopsidis/genome.fa 5422 sequences; 67,459,135 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABWE01001406.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 31 3.1 gb|ABWE01000083.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 30 4.1 gb|ABWE01002537.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 30 5.4 gb|ABWE01001049.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 30 5.4 gb|ABWE01000003.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 30 7.0 gb|ABWE01000153.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 29 9.2 >gb|ABWE01001406.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont190.3, whole genome shotgun sequence Length = 14270 Score = 30.8 bits (68), Expect = 3.1 Identities = 21/74 (28%), Positives = 37/74 (50%), Gaps = 9/74 (12%) Frame = +2 Query: 20 LCVLCGLPAAGKSTFARAL------AHRLQQEQGWAIGVVAYDDVMPDAFLAGARARPA- 72 L ++CGLPAAGK+T + L + RL + ++ DD+ + ++PA Sbjct: 10736 LVLVCGLPAAGKTTLVKQLVASGNTSSRLYER-------LSLDDLYEQQAIDDGISKPAE 10894 Query: 73 --PSQWKLLRQELL 84 P+ WK + E++ Sbjct: 10895 FDPNIWKASQHEMV 10936 >gb|ABWE01000083.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont159.8, whole genome shotgun sequence Length = 88009 Score = 30.4 bits (67), Expect = 4.1 Identities = 14/44 (31%), Positives = 21/44 (47%) Frame = -2 Query: 190 NGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPACAS 233 NG+R Q + + + RK + P K W +S P P+C S Sbjct: 62184 NGERIQKVTSKVERCLNRKTYQVCPVKRTWFPSSRVFPGPSCLS 62053 >gb|ABWE01002537.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont1188.2, whole genome shotgun sequence Length = 2988 Score = 30.0 bits (66), Expect = 5.4 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = -1 Query: 304 KQRWVRANHAAIWRIILGNEHIKCRSAKVGWLQ 336 +QRW+++ H +W+ L +E SA WLQ Sbjct: 2667 EQRWLKSQHGFLWQQALASELAGKNSAASRWLQ 2569 >gb|ABWE01001049.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont100.12, whole genome shotgun sequence Length = 20778 Score = 30.0 bits (66), Expect = 5.4 Identities = 21/69 (30%), Positives = 34/69 (49%) Frame = -2 Query: 184 ETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPACASEASLEVTDLL 243 E+ LQ + P A P ETIH+ + P+ + L +PSP C + A ++ +LL Sbjct: 11990 ESALQASSPTP-APPLETIHVFYMTGDGPSS-------HPLQVPSPPCYASAITQLPELL 11835 Query: 244 LTALENPVK 252 L + +K Sbjct: 11834 LDEMLRALK 11808 >gb|ABWE01000003.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont14.19, whole genome shotgun sequence Length = 194456 Score = 29.6 bits (65), Expect = 7.0 Identities = 18/55 (32%), Positives = 27/55 (49%), Gaps = 6/55 (10%) Frame = -1 Query: 189 RNGQRPQALPPETI---HLMGRKLEKPNPEKNAWEHNSLTIPS---PACASEASL 237 R+ + + LPP+ H EK +P AW H ++ +PS ACAS S+ Sbjct: 2399 RSKRAARWLPPQNALGSHCKANSDEKESPSTCAWRHQTVLLPSISDIACASVGSV 2235 >gb|ABWE01000153.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont76.8, whole genome shotgun sequence Length = 69636 Score = 29.3 bits (64), Expect = 9.2 Identities = 22/101 (21%), Positives = 42/101 (41%), Gaps = 4/101 (3%) Frame = -3 Query: 231 CASEASLEVTD----LLLTALENPVKYAEDNMEQKDTDRIICSTNILHKTDQTLRRIVSQ 286 CA + ++ + +LT ++ + + D +DT S N + +V Sbjct: 6958 CAPKRAISTVEPDGCAILTVYKSNLAASSDASRVEDTKATALSRNERTMSSVKKEEVVGG 6779 Query: 287 TMKEAKGNQEAFSEMTFKQRWVRANHAAIWRIILGNEHIKC 327 ++ A +E SEMT ++RW R A+ + +KC Sbjct: 6778 EVELALRVKEMNSEMTSEERWSRRRGFAVATVTAKLAAVKC 6656 Database: H.arabidopsidis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 67,459,135 Number of sequences in database: 5422 Lambda K H 0.321 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 31,495,285 Number of Sequences: 5422 Number of extensions: 497181 Number of successful extensions: 2585 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 590 Number of HSP's successfully gapped in prelim test: 151 Number of HSP's that attempted gapping in prelim test: 1830 Number of HSP's gapped (non-prelim): 1165 length of query: 348 length of database: 22,486,378 effective HSP length: 106 effective length of query: 242 effective length of database: 21,911,646 effective search space: 5302618332 effective search space used: 5302618332 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 64 (29.3 bits)