TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|116242735|sp|Q8IV42.2|PSTK_HUMAN RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase (348 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668657.1| Blastocystis hominis, Singapore isolate B (sub-t... 39 0.004 emb|FN668660.1| Blastocystis hominis, Singapore isolate B (sub-t... 31 0.93 emb|FN668689.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 1.2 emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t... 29 3.5 emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-t... 29 3.5 emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-t... 29 3.5 emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-t... 28 7.9 >emb|FN668657.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_26 Length = 145118 Score = 38.5 bits (88), Expect = 0.004 Identities = 25/92 (27%), Positives = 48/92 (52%), Gaps = 4/92 (4%) Frame = +3 Query: 127 AAFEAQSCYLLTKTAVSRPL----FLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCP 182 AAF+ ++ + + KT VSR L +++LD + + RYE+Y +AR C +++D P Sbjct: 69489 AAFDEKNTHEVIKTQVSRILRSDNYVILDTMNFIKGYRYEMYCIARCCKSTRCVVWVDTP 69668 Query: 183 LETCLQRNGQRPQALPPETIHLMGRKLEKPNP 214 + + ++ Q E + + + E P+P Sbjct: 69669 I-SAIESFNQSCSHYSNEVLQDLACRFEPPSP 69761 >emb|FN668660.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_29 Length = 89994 Score = 30.8 bits (68), Expect = 0.93 Identities = 28/116 (24%), Positives = 46/116 (39%), Gaps = 16/116 (13%) Frame = +1 Query: 212 PNP-----EKNAWEHNSLTIPSPACASEASLEVTD---LLLTALENPVKYAEDNMEQKDT 263 PNP + +AW+ SL +P C+S S + D +L + P A + T Sbjct: 50155 PNPPPFFSQDSAWDFLSLFLPRWICSSTRSFSIFDPVEAILRCVSLPPT*AMPLLRSLST 50334 Query: 264 DRIICSTNILHKTDQTLRRIVSQTMKEAKGNQEA--------FSEMTFKQRWVRAN 311 +CS + K++ T R + A ++ F M F R +R+N Sbjct: 50335 AWFLCSNILSPKSESTFPRFLHFNFGSAFSISDSFIISCPIFFLPMAFTGRSIRSN 50502 >emb|FN668689.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_7 Length = 870417 Score = 30.4 bits (67), Expect = 1.2 Identities = 35/165 (21%), Positives = 67/165 (40%), Gaps = 1/165 (0%) Frame = +3 Query: 23 LCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMPDAFLAGARARPAPSQWK-LLRQ 81 LCG PA GKST A L L+ E + ++YD + R R S+ K L + Sbjct: 81249 LCGAPATGKSTLASHLRDVLRPEAD--VFYISYDRI--------ERGRYERSEQKPLWER 81398 Query: 82 ELLKYLEYFLMAVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQSCYLLTKTA 141 E ++ + +++ + + + ED ++ + Sbjct: 81399 ETWRWARIHAIHILSDLLQILRSDGEGKIAEDILS-------------------YPARNR 81521 Query: 142 VSRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCPLETC 186 S+ +++DD + +SMR+ + +L + L C L + P++ C Sbjct: 81522 ESKLNVVIVDDTMHLRSMRHSIMKLCQICIL--CFLLIPRPVQLC 81650 Score = 29.3 bits (64), Expect = 2.7 Identities = 28/111 (25%), Positives = 44/111 (39%), Gaps = 18/111 (16%) Frame = +1 Query: 192 QRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTI---------------PSPACASEAS 236 QRP LPP + L R L P + W+ SL + SP + A Sbjct: 107251 QRPLHLPPHRLLLPHRHLRSARPRRPRWQRISLLVRIEEPLNLAAQRLYASSPLPTATAR 107430 Query: 237 LEVT-DLLLTALENPVKYA--EDNMEQKDTDRIICSTNILHKTDQTLRRIV 284 L + + L + E P+ A E + Q+D ++ N H D+ + R + Sbjct: 107431 LHASIERLHPSREIPLAVARTESLVHQQDDAEVVLVPN--HAADRLVHRAI 107577 >emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_1 Length = 1866585 Score = 28.9 bits (63), Expect = 3.5 Identities = 16/34 (47%), Positives = 19/34 (55%) Frame = +2 Query: 23 LCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYD 56 + G P AGKSTF AL L QG + V+A D Sbjct: 902531 IAGPPGAGKSTFCEALG-TLLTSQGHRVAVLAID 902629 >emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_2 Length = 1458350 Score = 28.9 bits (63), Expect = 3.5 Identities = 18/67 (26%), Positives = 29/67 (43%), Gaps = 5/67 (7%) Frame = +1 Query: 186 CLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAW-----EHNSLTIPSPACASEASLEVT 240 C R RP+ PP T H L P W +S + P+ C+ +AS ++ Sbjct: 818878 CTPRRAARPRGWPPSTSHF----LSTPTIIPATWGLLLSSESSSSSPNTPCSQDASSLMS 819045 Query: 241 DLLLTAL 247 ++ TA+ Sbjct: 819046 LIICTAI 819066 >emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_4 Length = 1031885 Score = 28.9 bits (63), Expect = 3.5 Identities = 18/67 (26%), Positives = 29/67 (43%), Gaps = 5/67 (7%) Frame = +2 Query: 186 CLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAW-----EHNSLTIPSPACASEASLEVT 240 C R RP+ PP T H L P W +S + P+ C+ +AS ++ Sbjct: 262343 CTPRRAARPRGWPPSTSHF----LSTPTIIPATWGLLLSSESSSSSPNTPCSQDASSLMS 262510 Query: 241 DLLLTAL 247 ++ TA+ Sbjct: 262511 LIICTAI 262531 >emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_0 Length = 1966360 Score = 27.7 bits (60), Expect = 7.9 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = -2 Query: 101 SVPPNRTEAMWEDFITCLKDQDLIFSA 127 S PPNR+ A+ F +C ++ +FSA Sbjct: 86640 STPPNRSSALGPSFSSCQSNKVFLFSA 86560 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.321 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,628,796 Number of Sequences: 54 Number of extensions: 140279 Number of successful extensions: 724 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 661 Number of HSP's gapped (non-prelim): 192 length of query: 348 length of database: 6,272,415 effective HSP length: 97 effective length of query: 251 effective length of database: 6,267,177 effective search space: 1573061427 effective search space used: 1573061427 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 59 (27.3 bits)