TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor (eEFSec) # Homo sapiens # Complete (596 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 354 9e-98 gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 92 6e-19 gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 70 3e-12 gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 68 2e-11 gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 44 2e-04 gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 42 8e-04 gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 42 0.001 gb|CM000626.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 38 0.014 gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 37 0.025 gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 33 0.36 gb|CM000624.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 31 2.3 >gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun sequence Length = 2535400 Score = 354 bits (908), Expect = 9e-98 Identities = 214/592 (36%), Positives = 310/592 (52%), Gaps = 52/592 (8%) Frame = +2 Query: 13 LGHIDSGKXXXXXXXXXXXXXXXFDKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXX 72 LGH+DSGK DK QSR+RG+TLDLGFSCF + +P+ L++S Sbjct: 950741 LGHVDSGKTSLVKALSTLLSTAALDKSKQSRQRGMTLDLGFSCFFLDMPSHLQASYPDKK 950920 Query: 73 XXXXXXXXXXXXXXQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLV 132 Q+TLVDCPGHASLIRTIIGGAQIID+++LV+D KG Q Q+ ECLV Sbjct: 950921 NL------------QITLVDCPGHASLIRTIIGGAQIIDMVLLVVDAVKGWQAQTTECLV 951064 Query: 133 IGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPV-------- 184 + ++ LVV LNK D P +R +++ K++ L+ T+F API+ V Sbjct: 951065 LAELTSPYLVVALNKADQFPPPERDQLLEQAAAKIRDRLKATRFANAPIVGVAACVGGEK 951244 Query: 185 --XXXXXXXXXXXXXXXXXIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMT 242 + L++ L ++ P R P G F SVDHCFSI+G+GTV+T Sbjct: 951245 VAAASEDMSKLEVRNETYNMETLVQTLQDKLPPPKRTPEGSFYFSVDHCFSIRGRGTVLT 951424 Query: 243 GTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGL 302 GT+L+G+ S+ D +E P L + +K+KS+QMF + QGDR G+CV+ D KLLERG+ Sbjct: 951425 GTVLNGAASVNDVLEFPTLGLDRKIKSIQMFKRQVQRIQQGDRAGVCVSNLDAKLLERGI 951604 Query: 303 VCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFF----------S 352 P ++ + A+ V K+PY+ G L + +KFHI+VGH T+M + F+ + Sbjct: 951605 AATPGAVQLLKGAIALVRKVPYYVGTLHSGSKFHISVGHTTIMATVTFWGAVELLGKEQN 951784 Query: 353 PAP----DNFDQEPILDSFNFS-------------QEYLFQEQYLSKDLTPAVTDNDEAD 395 PA + D +P S + F+E+++ +D D + Sbjct: 951785 PADLSVVNPVDTKPSKRYVGSSSLGGDADMAGLPRMTFSFEEEFIHQD--------DLLE 951940 Query: 396 KKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHT----NTCRLAFHGILL 451 GQ + H WAL++F+ PV CP LVIGSRLD ++ + CRLAF G L+ Sbjct: 951941 SLDGQESLLH-----WALLDFQIPVHCPMDSLVIGSRLDTVDNSTGSASACRLAFSGRLM 952105 Query: 452 HGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDD-----------YSVIGRSLFKKETNI 500 ++ + +L++Y K + G + R D Y V G LFKKETN+ Sbjct: 952106 QSVD----PGKDISKLRLYTPKERRGTISRLGDPHKRTDDDKLVRYEVFGSDLFKKETNM 952273 Query: 501 QLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRA 552 + F G+KV E+G I S+FG SGK+++ P G + + L K+ A Sbjct: 952274 KAFFGMKVQTERNEIGEIKSSFGTSGKYRVFFPAGTEAKEGEPLILRFKRFA 952429 Score = 35.4 bits (80), Expect = 0.094 Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 7/69 (10%) Frame = +1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQ-------K 140 VT +D PGHA+ G+ D+++LVI G+ Q+ E L + Sbjct: 742393 VTFLDTPGHAAFKTMRQSGSDAADILVLVIAADDGVSEQTVEILDFYKSIITSSGGSGIS 742572 Query: 141 LVVVLNKID 149 LV+ +NKID Sbjct: 742573 LVIAMNKID 742599 >gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 21, whole genome shotgun sequence Length = 662217 Score = 92.4 bits (228), Expect = 6e-19 Identities = 65/224 (29%), Positives = 112/224 (50%), Gaps = 6/224 (2%) Frame = +3 Query: 94 PGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIG-QIACQKLVVVLNKIDLLP 152 PGHA ++ +I GA +D +LV+ T G Q+ E +++ Q+ LVV LNK+DL+ Sbjct: 404019 PGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLAKQVGIPNLVVFLNKVDLVD 404198 Query: 153 EGKRQAAIDKMTKKMQKTLENTKFRG--APIIPVXXXXXXXXXXXXXXXXXIPELIELLT 210 + + ++ + ++++ L+ F G PII I EL+ + Sbjct: 404199 D---EELLELVEMEIRELLDFYDFPGDDIPIIRGSALAAAEGRNPEIGSEKILELMAAVD 404369 Query: 211 SQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKV--K 268 +I P RD FLM ++ FSI G+GTV+TG + G +++GD +++ L K Sbjct: 404370 EKIPEPMRDLDKDFLMPIEDVFSIAGRGTVVTGRVQQGKVNVGDELDVIGLDQNHKTICT 404549 Query: 269 SMQMFHMPITSAMQGDRLGICVTQFDPKLLERG-LVCAPESLHT 311 ++MF + M GD +G + + + RG ++C P S+ T Sbjct: 404550 GVEMFKKLLDFGMAGDNIGALLRGLKREDVRRGQILCKPGSMKT 404681 >gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 13, whole genome shotgun sequence Length = 887524 Score = 70.1 bits (170), Expect = 3e-12 Identities = 64/282 (22%), Positives = 111/282 (39%), Gaps = 13/282 (4%) Frame = +3 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 D RERGIT+D+ F P T++D PGH Sbjct: 601719 DNLKAERERGITIDIALWKFESP-------------------------KYSFTVIDAPGH 601823 Query: 97 ASLIRTIIGGAQIIDLMMLVID---------VTKGMQTQSAECLVIGQIACQKLVVVLNK 147 I+ +I G D+ +LVID ++K QT+ L+ + ++++V +NK Sbjct: 601824 RDFIKNMITGTSQADVAVLVIDSSQGGFEAGISKDGQTRE-HALLAYTLGVKQMIVAMNK 602000 Query: 148 IDLLPEGKRQAAIDKMTKKMQKTLENTKFR--GAPIIPVXXXXXXXXXXXXXXXXXI--P 203 +D + ++ ++ L+ ++ P +P+ P Sbjct: 602001 MDDKTVKYAEDRYTEIKNEVSAYLKKVGYKPMKIPFVPISGWEGDNMVEKSTNMAWYKGP 602180 Query: 204 ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263 L+E L S ++ P R + + + I G GTV G + +G I G + Sbjct: 602181 YLLEALDS-VTPPKRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVIKPGIHAMFAPSGI 602357 Query: 264 VKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305 + ++KS++M H + A+ GD +G V K L RG V + Sbjct: 602358 IAEIKSVEMHHESLPEAVPGDNVGFNVKNVAVKDLRRGFVAS 602483 >gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 23, whole genome shotgun sequence Length = 512847 Score = 67.8 bits (164), Expect = 2e-11 Identities = 62/232 (26%), Positives = 109/232 (46%), Gaps = 22/232 (9%) Frame = +2 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQI-ACQKLVVVL 145 V+ VDCPGH L+ T++ GA ++D +L+I + Q Q++E L +I + ++++ Sbjct: 45905 VSFVDCPGHDILMATMLNGAAVMDAALLLIAGNETCPQPQTSEHLAAVEIMRLEHILILQ 46084 Query: 146 NKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPEL 205 NK+DL+ K AA+ + ++++K + T API+P+ + L Sbjct: 46085 NKVDLV---KPDAAVAQQ-EQIRKFVAGTVADAAPILPI----------SAVLRYNMDVL 46222 Query: 206 IELLTSQISIPTRDPSGPFLMSVDHCFSI--------KGQGTVMTGTILSGSISLGDSVE 257 E L +I +P RD + + V F + K QG V G+IL G + +GD +E Sbjct: 46223 CEYLIRRIPLPVRDFTSQPRLIVIRSFDVNRPGQDVSKLQGGVAGGSILQGVLRVGDEIE 46402 Query: 258 ------------IPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKL 297 I + + K+ S+ + A+ G +G+ T+ DP L Sbjct: 46403 VRPGIVHKQDDKIVCVPIFSKISSLYAEQNDLQFAVPGGLIGV-GTKIDPTL 46555 >gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 5, whole genome shotgun sequence Length = 1098047 Score = 44.3 bits (103), Expect = 2e-04 Identities = 52/225 (23%), Positives = 88/225 (39%), Gaps = 27/225 (12%) Frame = -1 Query: 89 TLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM---------QTQSAECLVIGQIACQ 139 T++D PGH + + +I GA D+ +LVI KG QT+ ++ + Sbjct: 710684 TILDAPGHKNYVPNMIQGASQADVGVLVISARKGEFETGFDRGGQTRE-HAMLAKTLGVS 710508 Query: 140 KLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKF---RGAPIIPV------------ 184 LVV +NK+D ++ D+ K++ L++ F R IP+ Sbjct: 710507 YLVVAVNKMDEPTVQWKKERFDECVTKIRPFLKSCGFVIKREVKFIPISGLSGANVKDEV 710328 Query: 185 ---XXXXXXXXXXXXXXXXXIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVM 241 P L+ LL + + I RDP P + V ++ +G + Sbjct: 710327 ALSECSWWKEYYTSNENNTSAPTLLSLLDT-LEIAGRDPEAPLRIPVLDRYT--DRGIIS 710157 Query: 242 TGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRL 286 G + SG I G V I + KV + P++ A G+ + Sbjct: 710156 MGKVESGIIRPGMKVTIMPTRNKYKVDEVWANEDPVSGARPGENV 710022 >gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 18, whole genome shotgun sequence Length = 702471 Score = 42.4 bits (98), Expect = 8e-04 Identities = 37/200 (18%), Positives = 79/200 (39%), Gaps = 3/200 (1%) Frame = -3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + + +LV+D ++G++ Q+ + + +++ +LNK Sbjct: 516373 LNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGIEAQTLANVYLALENDLEIIPILNK 516194 Query: 148 IDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPELIE 207 IDL A +++ ++++ T + I +++E Sbjct: 516193 IDL-----PAADPERVAEEIEAT-------------IGLDCSGIVHASAKTGIGIDDILE 516068 Query: 208 LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV---V 264 + + P GPF + + +G ++ ++ G +S GD V A V Sbjct: 516067 RIVQMVPPPPAATGGPFRALIFDSYYDAYRGVIVFFRVMDGEVSQGDKVRFLASDAEHDV 515888 Query: 265 KKVKSMQMFHMPITSAMQGD 284 +V MQ +P+ G+ Sbjct: 515887 TEVGIMQPNQVPVDCLHAGE 515828 Score = 39.3 bits (90), Expect = 0.006 Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 7/71 (9%) Frame = +1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE-------CLVIGQIACQK 140 V L D PGHA + +I G D +LV+D T G + + V + + Sbjct: 619192 VVLQDAPGHADYVPAMITGTASADAALLVVDATDGAAHTALQSGQLKEHAYVARGLGVNQ 619371 Query: 141 LVVVLNKIDLL 151 ++VV+NK+DL+ Sbjct: 619372 ILVVINKMDLV 619404 Score = 30.8 bits (68), Expect = 2.3 Identities = 14/46 (30%), Positives = 25/46 (54%) Frame = +2 Query: 544 LTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTH 589 + P++KK R + A R++ +A R S V + K+Y+ D+H Sbjct: 126701 VAPSIKKYRRPRQNAAIREKTAAPRQTRSTSVCCKIHSKKYLLDSH 126838 >gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 27, whole genome shotgun sequence Length = 404298 Score = 42.0 bits (97), Expect = 0.001 Identities = 20/60 (33%), Positives = 34/60 (56%) Frame = -2 Query: 90 LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149 ++D PGH S G+ + D+ +LV+D+ G++ Q+ E L + + VV LNK+D Sbjct: 276575 VIDTPGHESFTNLRSRGSSLCDVAILVVDLMHGLEQQTIESLTMLRKRGVPFVVALNKVD 276396 >gb|CM000626.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 24, whole genome shotgun sequence Length = 511739 Score = 38.1 bits (87), Expect = 0.014 Identities = 23/62 (37%), Positives = 31/62 (50%) Frame = +3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 V VD PGHA + ++D ++LV+D +G TQ+ L K VVVLNK Sbjct: 474183 VNCVDTPGHADFCGEVDRILSMVDGVVLVVDAAEGPMTQTKYVLSRALTLGLKPVVVLNK 474362 Query: 148 ID 149 D Sbjct: 474363 CD 474368 >gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 8, whole genome shotgun sequence Length = 1007773 Score = 37.4 bits (85), Expect = 0.025 Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 2/66 (3%) Frame = +1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLV--VVL 145 + L D PGH R + D +LV+D +GM ++ + V + Q+LV +VL Sbjct: 142591 IHLYDSPGHTDFSREVSSAMSCCDTALLVVDAVEGMGPRTHQ--VFREAYAQQLVPILVL 142764 Query: 146 NKIDLL 151 NKID L Sbjct: 142765 NKIDRL 142782 Score = 33.5 bits (75), Expect = 0.36 Identities = 16/62 (25%), Positives = 30/62 (48%) Frame = +1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + ++ D ++V+D G+ Q+ L K V+++NK Sbjct: 702361 INLIDSPGHVDFSSEVTAALRVTDGALVVVDTIDGVCVQTETVLRQAIAERVKPVLMVNK 702540 Query: 148 ID 149 +D Sbjct: 702541 VD 702546 >gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 6, whole genome shotgun sequence Length = 1035082 Score = 33.5 bits (75), Expect = 0.36 Identities = 18/66 (27%), Positives = 36/66 (54%), Gaps = 2/66 (3%) Frame = +2 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQ--KLVVVL 145 ++++DCPGH + + D ++V+DV +G+ + +V+ Q + L +VL Sbjct: 829937 ISMLDCPGHVQFHDESVAALKASDGAVVVVDVVEGIMMHTE--MVVRQAISEGLSLTLVL 830110 Query: 146 NKIDLL 151 +K+D L Sbjct: 830111 SKMDRL 830128 >gb|CM000624.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 22, whole genome shotgun sequence Length = 591336 Score = 30.8 bits (68), Expect = 2.3 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = -3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQS 127 + L+D PGH + ++D +LV+D G+Q Q+ Sbjct: 303304 IALIDTPGHVDFSVEVNRSVAVLDGAVLVVDAVAGVQAQT 303185 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,179,184 Number of Sequences: 31 Number of extensions: 201860 Number of successful extensions: 931 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 822 Number of HSP's gapped (non-prelim): 197 length of query: 596 length of database: 7,911,228 effective HSP length: 104 effective length of query: 492 effective length of database: 7,908,004 effective search space: 3890737968 effective search space used: 3890737968 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 63 (28.9 bits)