TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor (eEFSec) # Homo sapiens # Complete (596 letters) Database: N.gruberi/genome.fa 784 sequences; 40,964,085 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 252 1e-70 gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 111 2e-24 gb|GG738872.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 67 6e-11 gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 67 6e-11 gb|GG738913.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 64 3e-10 gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 64 3e-10 gb|GG738869.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 64 3e-10 gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 59 2e-08 gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 47 7e-05 gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 45 3e-04 gb|GG738893.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 42 0.002 gb|GG738899.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 38 0.032 gb|GG738894.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 38 0.032 gb|GG738871.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 38 0.032 gb|GG738859.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 37 0.055 gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 36 0.094 gb|GG738907.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 32 1.4 gb|GG738873.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 32 1.4 gb|GG738931.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 30 5.1 gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 30 5.1 >gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_11, whole genome shotgun sequence Length = 613270 Score = 252 bits (644), Expect(2) = 1e-70 Identities = 136/317 (42%), Positives = 188/317 (59%), Gaps = 16/317 (5%) Frame = -1 Query: 36 FDKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPG 95 FDK PQS+ERGIT DLGFS F P Q TLVDCPG Sbjct: 66814 FDKSPQSKERGITQDLGFSAFYSDSPLS------------------EYDRIQYTLVDCPG 66689 Query: 96 HASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGK 155 HASLIRTIIGGAQIID M+LVID KG+QTQ+AEC+VIG+I+C L+V LNK+D++P + Sbjct: 66688 HASLIRTIIGGAQIIDFMVLVIDAVKGVQTQTAECIVIGEISCNNLIVALNKVDMIPADQ 66509 Query: 156 RQAAIDKMTK-----------KMQKTL-----ENTKFRGAPIIPVXXXXXXXXXXXXXXX 199 R+ +DK++ +++K+L TKF+ PI+ V Sbjct: 66508 REDRVDKVSNFKIYLGN*FSLQIKKSLIKNVFSQTKFKNPPIVSVCADPREESESITKEQ 66329 Query: 200 XXIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIP 259 + LI + I +D + PFL VDHCF+IKG+GT++TGT+L GS+S+G +++IP Sbjct: 66328 G-LKNLIAEFQKHVKISEKDENEPFLYMVDHCFTIKGKGTIITGTVLQGSVSIGKTIQIP 66152 Query: 260 ALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISV 319 ++ KKVKS+QMF P+ A++GDR+GI VT+ D K LERG C + V L + Sbjct: 66151 SIGEEKKVKSIQMFKTPVDKAIKGDRIGIAVTKLDSKKLERGFACDKGYIEIVDTILANA 65972 Query: 320 EKIPYFRGPLQTKAKFH 336 +I +F+ ++ AK+H Sbjct: 65971 SRIRFFKKEIKNSAKYH 65921 Score = 66.6 bits (161), Expect = 6e-11 Identities = 38/100 (38%), Positives = 60/100 (60%), Gaps = 6/100 (6%) Frame = -3 Query: 437 IHTNTCRLAFHGILLHGLEDRNYA-DSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFK 495 I N CRLAF+G ++ N + + + L V+K K K +V+R +D ++IG++L Sbjct: 65615 IDANMCRLAFNGKVIKMWNKSNVSKEQLVNSLPVFKYKEKKAVVDRVVDANTIIGKNLLT 65436 Query: 496 KET-NIQLFVGLKVHLSTGEL----GIIDSAFGQSGKFKI 530 +T ++Q F+G K+ + + GIIDS FGQSGKFK+ Sbjct: 65435 SKTQSLQFFIGKKIDILIDDQPRFSGIIDSPFGQSGKFKV 65316 Score = 34.3 bits (77), Expect(2) = 1e-70 Identities = 33/122 (27%), Positives = 48/122 (39%), Gaps = 9/122 (7%) Frame = -2 Query: 337 ITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADK 396 +++GHETVMG + + A I D+ + EY E+ +T+ +K Sbjct: 65880 VSIGHETVMGNVSLIARAKKEGLSSNIFDT---TVEYSLLEE---------LTETTYQEK 65737 Query: 397 KAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADI---------HTNTCRLAFH 447 + +EFEKPV C I S+LD DI +TN C Sbjct: 65736 DV------------FVKIEFEKPVCCYPNSNYIASKLDTDISKFFTIIFSNTNRCEYV*I 65593 Query: 448 GI 449 GI Sbjct: 65592 GI 65587 >gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun sequence Length = 707703 Score = 111 bits (277), Expect = 2e-24 Identities = 75/225 (33%), Positives = 117/225 (52%), Gaps = 9/225 (4%) Frame = -2 Query: 91 VDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVI-GQIACQKLVVVLNKID 149 +DCPGHA I+ +I GA +D +LVI G Q+ E L++ Q+ + +VV LNK+D Sbjct: 506243 IDCPGHADYIKNMITGAAQMDGSILVISADDGPMLQTREHLLLCKQVGIKHIVVFLNKVD 506064 Query: 150 LLPEGKRQAAIDKMTKKMQKTLENTKFRG--APIIPVXXXXXXXXXXXXXXXXXIPELIE 207 L+P+ + I+ + ++++ T++ F G II I +L++ Sbjct: 506063 LVPDAEM---IEMVEEEIRDTVKQYGFDGDNISIIRGSALKGLEGDSSEIGTQAIQKLMD 505893 Query: 208 LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI-----PALK 262 + I P RD + PFLM V+ F+I G+GTV TG + G + LG VE+ ALK Sbjct: 505892 AVDKDIPEPERDINKPFLMPVEGVFTISGRGTVATGKVDQGQLKLGAEVEVVGFAPTALK 505713 Query: 263 VVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERG-LVCAP 306 V ++MFH + GD LGI + D K ++RG ++C P Sbjct: 505712 --SSVTGIEMFHKLVDRGQAGDNLGILLRGIDKKQIKRGQVICKP 505584 Score = 29.6 bits (65), Expect = 8.8 Identities = 24/79 (30%), Positives = 40/79 (50%), Gaps = 1/79 (1%) Frame = +2 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKR 156 +SL TI +++ + VID +K Q S ECL +I + ++D L E + Sbjct: 451892 SSLSSTINSEDELLKKVFKVIDESKQQQQNSRECL--NKIMIDHEHALSIQLDNLSEIQT 452065 Query: 157 Q-AAIDKMTKKMQKTLENT 174 Q +I+K ++ KT+ NT Sbjct: 452066 QIQSIEKRAVQLGKTISNT 452122 >gb|GG738872.1| Naegleria gruberi genomic scaffold NAEGRscaffold_28, whole genome shotgun sequence Length = 413107 Score = 66.6 bits (161), Expect = 6e-11 Identities = 58/202 (28%), Positives = 97/202 (48%), Gaps = 10/202 (4%) Frame = +3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL- 145 V+ VDCPGH L+ T++ GA ++D +L+I + Q Q++E L +I K +++L Sbjct: 113118 VSFVDCPGHDILMATMLNGAAVMDAALLLIAGNEPCPQPQTSEHLAAVEIMKLKHIIILQ 113297 Query: 146 NKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPEL 205 NKIDL+ E D++ + ++ T+ + +PIIP+ I + Sbjct: 113298 NKIDLVKESDALNQFDEIKRFVKGTVAD----NSPIIPI----------SAQLKYNIDVV 113435 Query: 206 IELLTSQISIPTRDPSGPFLMSVDHCFSIKG--------QGTVMTGTILSGSISLGDSVE 257 E + +I IP RD + + V F + +G V G+IL G + +GD +E Sbjct: 113436 CEHIVKRIPIPIRDFTSSPRLIVIRSFDVNKPGEAVENLKGGVAGGSILRGVLRIGDEIE 113615 Query: 258 IPALKVVKKVKSMQMFHMPITS 279 + +V K K + PITS Sbjct: 113616 VRP-GIVTKEKDGTIKCKPITS 113678 Score = 42.4 bits (98), Expect = 0.001 Identities = 25/81 (30%), Positives = 40/81 (49%) Frame = +3 Query: 234 IKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQF 293 I G GT + G ++SGSI V+I ++ VKS+++FH T +GD G+ + Sbjct: 356040 IGGIGTTVIGRVISGSIQFDQEVKITET-ILSNVKSLEIFHENQTKVNRGDFCGVALKNV 356216 Query: 294 DPKLLERGLVCAPESLHTVHA 314 + RG + + HT A Sbjct: 356217 RMVDITRGSLIGIDPSHTFEA 356279 >gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_5, whole genome shotgun sequence Length = 850158 Score = 66.6 bits (161), Expect = 6e-11 Identities = 68/284 (23%), Positives = 114/284 (40%), Gaps = 14/284 (4%) Frame = -1 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 D+ + R+RG+T+D+G F P VT++D PGH Sbjct: 518343 DEHEEERQRGVTMDVGVRYFETP-------------------------NRHVTILDAPGH 518239 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTK-GMQT------QSAECLVIGQ-IACQKLVVVLNKI 148 I +I GA D +LVID T G +T Q+ E L++ + + ++++VV+NK+ Sbjct: 518238 KDFIPKMITGAAQADFAILVIDSTPGGFETGFANGGQTKEHLILARSLGVEQVIVVVNKL 518059 Query: 149 DLLPEGKRQAAIDKMTKKMQKTLENTKFR---GAPI--IPVXXXXXXXXXXXXXXXXXIP 203 D + G + D + ++ + F+ G+ + IP Sbjct: 518058 DSI--GWSKERYDSIVAQLDDFMRQIGFQTQDGSHVFYIPASGLQGENLITKSGSISWYD 517885 Query: 204 EL-IELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALK 262 L + + RD MSV + G + G I +G+IS GD + I ++ Sbjct: 517884 GLSVVERIDKFEPKPRDIEKALRMSVSDVYKSLATGITVAGKIETGTISEGDELVIMPIR 517705 Query: 263 VVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAP 306 V VKS+ P+ GD + I + D + L G + P Sbjct: 517704 EVCTVKSILRHKSPVKHGYAGDNVEIGLGSIDIEKLMVGQILCP 517573 Score = 32.3 bits (72), Expect = 1.4 Identities = 13/29 (44%), Positives = 20/29 (68%) Frame = -1 Query: 228 VDHCFSIKGQGTVMTGTILSGSISLGDSV 256 +D F + G GTV++G + SGSIS+ D + Sbjct: 621456 IDQTFFVSGVGTVVSGIVQSGSISVNDKL 621370 >gb|GG738913.1| Naegleria gruberi genomic scaffold NAEGRscaffold_69, whole genome shotgun sequence Length = 236320 Score = 64.3 bits (155), Expect = 3e-10 Identities = 68/287 (23%), Positives = 108/287 (37%), Gaps = 18/287 (6%) Frame = -2 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 DK RERGIT+D+ F T++D PGH Sbjct: 70614 DKLKAERERGITIDIALWKFE-------------------------SKKYVFTIIDAPGH 70510 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148 I+ +I G D+ +LV+D T G Q+ E L+ + ++++V +NK Sbjct: 70509 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 70330 Query: 149 D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVXXXXXXXXXXXXXXXXX 201 D + KR I + K+L G P +P+ Sbjct: 70329 DDTTVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 70150 Query: 202 I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258 P L++ L + + P R P + + + I G GTV G + +G + G V Sbjct: 70149 YKGPCLLDALDNLVE-PIRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMMVHFA 69973 Query: 259 PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305 P +VKS++M H + A GD +G V ++RG V + Sbjct: 69972 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 69832 >gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome shotgun sequence Length = 1001995 Score = 64.3 bits (155), Expect = 3e-10 Identities = 68/287 (23%), Positives = 108/287 (37%), Gaps = 18/287 (6%) Frame = -1 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 DK RERGIT+D+ F T++D PGH Sbjct: 892702 DKLKAERERGITIDIALWKFE-------------------------SKKYVFTIIDAPGH 892598 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148 I+ +I G D+ +LV+D T G Q+ E L+ + ++++V +NK Sbjct: 892597 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 892418 Query: 149 D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVXXXXXXXXXXXXXXXXX 201 D + KR I + K+L G P +P+ Sbjct: 892417 DDESVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 892238 Query: 202 I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258 P L++ L + + P R P + + + I G GTV G + +G + G V Sbjct: 892237 YKGPCLLDALDNLVE-PVRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMVVHFA 892061 Query: 259 PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305 P +VKS++M H + A GD +G V ++RG V + Sbjct: 892060 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 891920 >gb|GG738869.1| Naegleria gruberi genomic scaffold NAEGRscaffold_25, whole genome shotgun sequence Length = 747259 Score = 64.3 bits (155), Expect = 3e-10 Identities = 68/287 (23%), Positives = 108/287 (37%), Gaps = 18/287 (6%) Frame = +2 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 DK RERGIT+D+ F T++D PGH Sbjct: 181799 DKLKAERERGITIDIALWKFE-------------------------SKKYVFTIIDAPGH 181903 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148 I+ +I G D+ +LV+D T G Q+ E L+ + ++++V +NK Sbjct: 181904 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 182083 Query: 149 D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVXXXXXXXXXXXXXXXXX 201 D + KR I + K+L G P +P+ Sbjct: 182084 DDESVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 182263 Query: 202 I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258 P L++ L + + P R P + + + I G GTV G + +G + G V Sbjct: 182264 YKGPCLLDALDNLVE-PVRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMVVHFA 182440 Query: 259 PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305 P +VKS++M H + A GD +G V ++RG V + Sbjct: 182441 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 182581 >gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_62, whole genome shotgun sequence Length = 286411 Score = 58.5 bits (140), Expect = 2e-08 Identities = 58/236 (24%), Positives = 98/236 (41%), Gaps = 15/236 (6%) Frame = +3 Query: 89 TLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAECLVIGQ-IACQK 140 T++D PGH + + +I D+ +LVI KG Q+ E V+ + + + Sbjct: 151290 TILDAPGHKAYVPNMISATTQADVGILVISARKGEFEAGLEKGGQTMEHTVLSKTLGVDQ 151469 Query: 141 LVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFR---GAPIIPVXXXXXXXXXXXXX 197 L+VV+NKID K + D++ K+ TL+ + IP+ Sbjct: 151470 LIVVVNKIDDPSVAKDKVRYDEIVTKVSATLKGVGWNLKTQVQFIPIDALTGQNISSRNL 151649 Query: 198 XXXXI--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDS 255 P LIE L D + P + + + KG TV+ G +LSG I +G Sbjct: 151650 NVPWYSGPSLIEALDDIKLTAVHDVNAPLRLPIIDRYRDKG-CTVVLGKVLSGQIKIGQK 151826 Query: 256 VEIPALKVVKKVKSMQMFHMPITSAM--QGDRLGICVTQFDPKLLERGLVCAPESL 309 V + K V +V +++ + I + G+ + + + D + VC PE L Sbjct: 151827 VVVMPNKCVCEVIALKSHEINIHGGVVSAGENVELYLKGDDDNIASGHTVCDPEHL 151994 >gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_16, whole genome shotgun sequence Length = 536960 Score = 46.6 bits (109), Expect = 7e-05 Identities = 27/84 (32%), Positives = 44/84 (52%), Gaps = 2/84 (2%) Frame = +1 Query: 90 LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149 ++D PGH S G+ + D+ +LVID+ G++ Q+ E L + + +V LNK+D Sbjct: 14545 IIDTPGHESFNNLRSRGSSLCDIAILVIDIKHGLERQTIESLQLIKNRKTPFIVALNKVD 14724 Query: 150 LLPEGKR--QAAIDKMTKKMQKTL 171 L E K A I + K +K + Sbjct: 14725 ALYEWKATPNAPIRESLKNQKKNV 14796 >gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_19, whole genome shotgun sequence Length = 467654 Score = 44.7 bits (104), Expect = 3e-04 Identities = 36/168 (21%), Positives = 70/168 (41%), Gaps = 2/168 (1%) Frame = -3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 +T +D PGH + GAQ+ D+++LV+ G+ Q+ E + + A ++V +NK Sbjct: 215007 ITFIDTPGHEAFTSMRSCGAQVTDIIVLVVAADDGVMPQTREVIKHAREANVPMIVAINK 214828 Query: 148 IDLLPEGKRQAAIDKMTKKM--QKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPEL 205 ID K A ++ + + + + + K IP+ E+ Sbjct: 214827 ID-----KPNADVEMVERHLMNEGIVSEEKGGDTQFIPISALKGKGLKDLIEAIGLQAEM 214663 Query: 206 IELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLG 253 +EL ++P + +V C + G G T + G++ +G Sbjct: 214662 LELKAMVDNVPMQG-------TVIECKVLHGFGISATCIVRRGALKVG 214540 >gb|GG738893.1| Naegleria gruberi genomic scaffold NAEGRscaffold_49, whole genome shotgun sequence Length = 337165 Score = 42.0 bits (97), Expect = 0.002 Identities = 34/157 (21%), Positives = 63/157 (40%), Gaps = 11/157 (7%) Frame = -2 Query: 111 DLMMLVIDVTKG---------MQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAID 161 ++ +LV+DVTKG + + + LVV L K D +P + + ++ Sbjct: 261270 NICVLVVDVTKGVFEKDINNSIGNMRTHLQIAAAVGLTHLVVCLTKCDTIPVIELNSRLE 261091 Query: 162 KMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPELIELLTSQISIPTRDP- 220 ++ K++ L ++ P IP+ + +E + I + R Sbjct: 261090 EIRSKLKTIL---LWKNVPFIPICYDRDENIFKPTTGEFSKGKSLESVLKAIQVEPRKEW 260920 Query: 221 -SGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSV 256 P +M + + I G GTV+ G I++G I V Sbjct: 260919 IEKPLMMVIFQIYRIGGIGTVVAGKIITGKIKTDQQV 260809 Score = 37.7 bits (86), Expect = 0.032 Identities = 21/64 (32%), Positives = 34/64 (53%) Frame = +3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + A++ D ++++DV +G+ TQ+ L K +VLNK Sbjct: 279282 INLIDSPGHVDFSSEVSTAARLSDGCLVLVDVVEGVCTQTKTVLEQAFRENIKPCLVLNK 279461 Query: 148 IDLL 151 ID L Sbjct: 279462 IDRL 279473 >gb|GG738899.1| Naegleria gruberi genomic scaffold NAEGRscaffold_55, whole genome shotgun sequence Length = 331038 Score = 37.7 bits (86), Expect = 0.032 Identities = 22/68 (32%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = +2 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKL--VVVL 145 V L+DCPGH + +I D +L++D +G + Q+ VI Q +++ V+ + Sbjct: 171386 VNLIDCPGHIDFSSEVSTALRITDGAVLIVDAAEGCRGQTE--TVIRQSLQERIRPVLFI 171559 Query: 146 NKIDLLPE 153 NK+D L E Sbjct: 171560 NKVDRLIE 171583 >gb|GG738894.1| Naegleria gruberi genomic scaffold NAEGRscaffold_50, whole genome shotgun sequence Length = 499907 Score = 37.7 bits (86), Expect = 0.032 Identities = 22/68 (32%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = +2 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKL--VVVL 145 V L+DCPGH + +I D +L++D +G + Q+ VI Q +++ V+ + Sbjct: 137339 VNLIDCPGHIDFSSEVSTALRITDGAVLIVDAAEGCRGQTE--TVIRQSLQERIRPVLFI 137512 Query: 146 NKIDLLPE 153 NK+D L E Sbjct: 137513 NKVDRLIE 137536 Score = 37.7 bits (86), Expect = 0.032 Identities = 22/68 (32%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = -2 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKL--VVVL 145 V L+DCPGH + +I D +L++D +G + Q+ VI Q +++ V+ + Sbjct: 340666 VNLIDCPGHIDFSSEVSTALRITDGAVLIVDAAEGCRGQTE--TVIRQSLQERIRPVLFI 340493 Query: 146 NKIDLLPE 153 NK+D L E Sbjct: 340492 NKVDRLIE 340469 >gb|GG738871.1| Naegleria gruberi genomic scaffold NAEGRscaffold_27, whole genome shotgun sequence Length = 417575 Score = 37.7 bits (86), Expect = 0.032 Identities = 22/68 (32%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = -1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKL--VVVL 145 V L+DCPGH + +I D +L++D +G + Q+ VI Q +++ V+ + Sbjct: 242201 VNLIDCPGHIDFSSEVSTALRITDGAVLIVDAAEGCRGQTE--TVIRQSLQERIRPVLFI 242028 Query: 146 NKIDLLPE 153 NK+D L E Sbjct: 242027 NKVDRLIE 242004 >gb|GG738859.1| Naegleria gruberi genomic scaffold NAEGRscaffold_15, whole genome shotgun sequence Length = 530355 Score = 37.0 bits (84), Expect = 0.055 Identities = 21/66 (31%), Positives = 38/66 (57%), Gaps = 2/66 (3%) Frame = +3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVL-- 145 V ++D PGH + + +I D ++LV+D +G+ + + E +I Q ++L +VL Sbjct: 287706 VNIMDTPGHINFSDELTASMRISDGIVLVVDALEGVMSNTVE--IIKQAVQERLPIVLLI 287879 Query: 146 NKIDLL 151 NK+D L Sbjct: 287880 NKLDRL 287897 Score = 31.6 bits (70), Expect = 2.3 Identities = 19/66 (28%), Positives = 36/66 (54%), Gaps = 4/66 (6%) Frame = +2 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTK--GMQTQSAECLVIGQIACQK--LVV 143 + L+DCPG L+ + ++ D +++ D T G T+ L+ QI+ + L++ Sbjct: 380618 INLLDCPGRLELVSQVSTSFRLTDSCIMLFDSTSKLGKYTE----LITRQISQENIPLLI 380785 Query: 144 VLNKID 149 +LNK+D Sbjct: 380786 MLNKLD 380803 >gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_39, whole genome shotgun sequence Length = 371401 Score = 36.2 bits (82), Expect = 0.094 Identities = 16/62 (25%), Positives = 31/62 (50%) Frame = +1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+DCPGH + ++ D ++V+D G+ Q+ L + V+++NK Sbjct: 184621 INLIDCPGHVDFSSEVTAALRVTDGALVVVDAVSGVCVQTETVLRQALQERIRPVLMMNK 184800 Query: 148 ID 149 +D Sbjct: 184801 LD 184806 >gb|GG738907.1| Naegleria gruberi genomic scaffold NAEGRscaffold_63, whole genome shotgun sequence Length = 275174 Score = 32.3 bits (72), Expect = 1.4 Identities = 19/78 (24%), Positives = 38/78 (48%), Gaps = 7/78 (8%) Frame = -3 Query: 111 DLMMLVIDVTKGMQTQSAECLV--IGQIACQKL-----VVVLNKIDLLPEGKRQAAIDKM 163 D + V DV + +E +V + ++ C+++ + LNK+DLL + R+ I+K Sbjct: 59016 DTFINVCDVVDEKTVEQSERVVEQVIKLKCREINYFPVIFALNKVDLLNDSNREQVIEKY 58837 Query: 164 TKKMQKTLENTKFRGAPI 181 T + +E + + I Sbjct: 58836 TNLINSIIERNQLKNTAI 58783 >gb|GG738873.1| Naegleria gruberi genomic scaffold NAEGRscaffold_29, whole genome shotgun sequence Length = 461873 Score = 32.3 bits (72), Expect = 1.4 Identities = 25/113 (22%), Positives = 39/113 (34%) Frame = +1 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 D PQ +ERGIT+ F +V L+D PGH Sbjct: 408742 DYMPQEQERGITITSAAITFK-------------------------WKDHRVNLIDTPGH 408846 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149 + ++D + + DV G++ QS +V +NK+D Sbjct: 408847 VDFTFEVERSVSVLDGAVAIFDVVNGVEAQSKTVWRQADRYNVPRIVYMNKMD 409005 >gb|GG738931.1| Naegleria gruberi genomic scaffold NAEGRscaffold_87, whole genome shotgun sequence Length = 138333 Score = 30.4 bits (67), Expect = 5.1 Identities = 21/79 (26%), Positives = 36/79 (45%), Gaps = 7/79 (8%) Frame = -1 Query: 111 DLMMLVIDVTKGMQTQSAECLVIGQIACQK-------LVVVLNKIDLLPEGKRQAAIDKM 163 + +LV D+ + AE LV + + +V LNK+DLL E R I+K Sbjct: 51411 ECFVLVCDLVDSNSVEEAEPLVEQLVRVKDFEENSFPVVFALNKVDLLNETDRPITIEKF 51232 Query: 164 TKKMQKTLENTKFRGAPII 182 T ++ ++ ++ II Sbjct: 51231 TNQIATIIKQSQLTNTFII 51175 Score = 30.4 bits (67), Expect = 5.1 Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 7/79 (8%) Frame = +1 Query: 111 DLMMLVIDVTKGMQTQSAECLVIGQIACQK-------LVVVLNKIDLLPEGKRQAAIDKM 163 + +LV D+ + AE LV + + +V LNK+DLL E R I+K Sbjct: 88582 ECFVLVCDLADINSVEEAEPLVEQLVRVKDFEENSFPVVFALNKVDLLNETDRPITIEKF 88761 Query: 164 TKKMQKTLENTKFRGAPII 182 T ++ ++ T+ II Sbjct: 88762 TNQIATLIKQTQLTNTFII 88818 >gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_4, whole genome shotgun sequence Length = 712659 Score = 30.4 bits (67), Expect = 5.1 Identities = 16/55 (29%), Positives = 27/55 (49%) Frame = +1 Query: 366 SFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPV 420 +F SQ L+ K +T AVT +D ++ A Q+ RQ+ +F+K + Sbjct: 197095 NFLLSQTELYSHFMSKKSITSAVTSDDSTEENAAQSALQASQRQKQFTEDFDKEI 197259 Database: N.gruberi/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 40,964,085 Number of sequences in database: 784 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 21,657,557 Number of Sequences: 784 Number of extensions: 284975 Number of successful extensions: 1036 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 47 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 878 Number of HSP's gapped (non-prelim): 330 length of query: 596 length of database: 13,654,695 effective HSP length: 108 effective length of query: 488 effective length of database: 13,570,023 effective search space: 6622171224 effective search space used: 6622171224 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 65 (29.6 bits)