TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor (eEFSec) # Homo sapiens # Complete (596 letters) Database: E.siliculosus/genome.fa 35 sequences; 195,934,119 total letters Searching...................................done Score E Sequences producing significant alignments: (bits) Value emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genom... 84 2e-15 emb|FN649736.1| Ectocarpus siliculosus strain Ec 32, whole genom... 69 5e-11 emb|FN649731.1| Ectocarpus siliculosus strain Ec 32, whole genom... 41 0.014 emb|FN649740.1| Ectocarpus siliculosus strain Ec 32, whole genom... 34 1.7 emb|FN649745.1| Ectocarpus siliculosus strain Ec 32, whole genom... 33 4.9 emb|FN649741.1| Ectocarpus siliculosus strain Ec 32, whole genom... 32 6.4 >emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, unassigned sequences LGUn Length = 58155991 Score = 83.6 bits (205), Expect = 2e-15 Identities = 39/61 (63%), Positives = 50/61 (81%), Gaps = 3/61 (4%) Frame = +1 Query: 91 VDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQK---LVVVLNK 147 VDCPGHASLIRTIIGGAQI+D+M+LV+D TKG+Q Q+ EC+VIG+ ++VVLNK Sbjct: 17111767 VDCPGHASLIRTIIGGAQIMDMMLLVVDATKGIQAQTTECVVIGEAVMSSGADVIVVLNK 17111946 Query: 148 I 148 + Sbjct: 17111947 V 17111949 Score = 52.8 bits (125), Expect = 5e-06 Identities = 19/44 (43%), Positives = 32/44 (72%) Frame = +2 Query: 209 LTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISL 252 L+ + +P+R S P + DHCFS++GQGT++TGT+L+G + + Sbjct: 17112824 LSRRARVPSRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKV 17112955 Score = 50.8 bits (120), Expect = 2e-05 Identities = 68/270 (25%), Positives = 101/270 (37%), Gaps = 69/270 (25%) Frame = +2 Query: 306 PESLHTVHAALI--SVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPI 363 P H H + + ++P F G + A H+++GH T M FF + + + Sbjct: 17113688 PPPPHARHVCRLFPAPSQVPAFTGQCLSGAYCHVSLGHSTAMATATFFGASEL---KNGL 17113858 Query: 364 LDSFNFSQEYLF---------QEQYLSKDLTPAVTDNDEA-------------DKKAGQA 401 L F S + L Q Q LS + ++ EA + A Sbjct: 17113859 LGLFEHSADGLKALEALRDAGQGQDLSLNEIASLPFPSEAAFVQQTHLCGAGSENSIAGA 17114038 Query: 402 TEGHC----PRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNT---------------- 441 + C P Q+ + F PV P +V+GSRLD+ + Sbjct: 17114039 DDEVCVDERPLLQYCHLTFHTPVITPPSSVVLGSRLDSTSSSPNKTEAAHPTGETIKGLA 17114218 Query: 442 -----CRLAFHGILLH----------GLEDRNYADSF---LPRLKVYKLKHKHGLVERAM 483 CR+AFHG L+ G D A F +LK++ K K G+V R Sbjct: 17114219 AVQEHCRIAFHGRLVSMSADVVGTRKGPGDARAALDFGTQAGQLKLFTEKRKTGVVFRVT 17114398 Query: 484 DD-------YSVIGRSLFKKETNIQLFVGL 506 + V G+ LFKKETN+ FVG+ Sbjct: 17114399 PNDQTGGGAVEVFGKDLFKKETNMPPFVGM 17114488 Score = 47.0 bits (110), Expect = 2e-04 Identities = 23/64 (35%), Positives = 35/64 (54%) Frame = +1 Query: 262 KVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEK 321 +++ KS + P GDR+G+CV D KL+ERG+V P S+ + AA+ V K Sbjct: 17113321 RLILPPKSTEYSFPPTHLCRAGDRVGMCVASLDAKLMERGVVTTPGSVRPISAAIALVRK 17113500 Query: 322 IPYF 325 + F Sbjct: 17113501 VCRF 17113512 Score = 40.8 bits (94), Expect = 0.018 Identities = 18/62 (29%), Positives = 35/62 (56%) Frame = -3 Query: 90 LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149 ++D PGH S G+ + D+ +LV+D+ G++ Q+ E L + + V+ LNK+ Sbjct: 5639318 IIDTPGHESFTNLRSRGSSLCDIAILVVDLMHGLEPQTIESLNLLRSKRTPFVIALNKVG 5639139 Query: 150 LL 151 ++ Sbjct: 5639138 MV 5639133 Score = 40.4 bits (93), Expect = 0.023 Identities = 19/24 (79%), Positives = 20/24 (83%) Frame = +2 Query: 41 QSRERGITLDLGFSCFSVPLPARL 64 QSRERGITLDLGFS F+V LP L Sbjct: 17111201 QSRERGITLDLGFSAFTVSLPEHL 17111272 Score = 35.4 bits (80), Expect = 0.75 Identities = 15/25 (60%), Positives = 21/25 (84%) Frame = +3 Query: 256 VEIPALKVVKKVKSMQMFHMPITSA 280 VE+P+L++ KKVKSMQMF P++ A Sbjct: 17113185 VELPSLRLQKKVKSMQMFRKPVSIA 17113259 >emb|FN649736.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG11 Length = 5521395 Score = 69.3 bits (168), Expect = 5e-11 Identities = 64/280 (22%), Positives = 112/280 (40%), Gaps = 13/280 (4%) Frame = +1 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 D RERGIT+D+ F P T++D PGH Sbjct: 2322784 DNLKAERERGITIDIALWKFESP-------------------------KYNFTVIDAPGH 2322888 Query: 97 ASLIRTIIGGAQIIDLMMLVI---------DVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 I+ +I G D+ +LV+ ++K QT+ L+ + ++++V +NK Sbjct: 2322889 RDFIKNMITGTSQADVAVLVVASGVGEFEAGISKNGQTRE-HALLAFTLGVKQMIVCINK 2323065 Query: 148 IDLLPEGKRQAAIDKMTKKMQKTLENTKFRGA--PIIPVXXXXXXXXXXXXXXXXXI--P 203 +D + ++ +++ L+ ++ A P +P+ P Sbjct: 2323066 MDDSSVMYGEPRYTEIKEEVAIYLKKVGYKPAKIPFVPISGWAGDNMIDKSTNMPWYKGP 2323245 Query: 204 ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263 L+E L + + PTR P + + + I G GTV G + +G + G V + Sbjct: 2323246 YLLEALDT-MKEPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGCLKPGMVVTFAPCML 2323422 Query: 264 VKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLV 303 +VKS++M H + A+ GD +G V K + RG V Sbjct: 2323423 DTEVKSVEMHHEALPEAVPGDNVGFNVKNVSVKDIRRGYV 2323542 Score = 69.3 bits (168), Expect = 5e-11 Identities = 64/280 (22%), Positives = 112/280 (40%), Gaps = 13/280 (4%) Frame = -2 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 D RERGIT+D+ F P T++D PGH Sbjct: 2318396 DNLKAERERGITIDIALWKFESP-------------------------KYNFTVIDAPGH 2318292 Query: 97 ASLIRTIIGGAQIIDLMMLVI---------DVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 I+ +I G D+ +LV+ ++K QT+ L+ + ++++V +NK Sbjct: 2318291 RDFIKNMITGTSQADVAVLVVASGVGEFEAGISKNGQTRE-HALLAFTLGVKQMIVCINK 2318115 Query: 148 IDLLPEGKRQAAIDKMTKKMQKTLENTKFRGA--PIIPVXXXXXXXXXXXXXXXXXI--P 203 +D + ++ +++ L+ ++ A P +P+ P Sbjct: 2318114 MDDSSVMYGEPRYTEIKEEVAIYLKKVGYKPAKIPFVPISGWAGDNMIDKSTNMPWYKGP 2317935 Query: 204 ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263 L+E L + + PTR P + + + I G GTV G + +G + G V + Sbjct: 2317934 YLLEALDT-MKEPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGCLKPGMVVTFAPCML 2317758 Query: 264 VKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLV 303 +VKS++M H + A+ GD +G V K + RG V Sbjct: 2317757 DTEVKSVEMHHEALPEAVPGDNVGFNVKNVSVKDIRRGYV 2317638 >emb|FN649731.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG06 Length = 3939081 Score = 41.2 bits (95), Expect = 0.014 Identities = 18/34 (52%), Positives = 24/34 (70%) Frame = -3 Query: 223 PFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSV 256 PFLM V+ FSI G+GTV+TG + G + +GD V Sbjct: 1610707 PFLMPVEDVFSIAGRGTVVTGRVEQGVVKVGDEV 1610606 >emb|FN649740.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG15 Length = 5788546 Score = 34.3 bits (77), Expect = 1.7 Identities = 22/65 (33%), Positives = 34/65 (52%) Frame = -1 Query: 529 KIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDT 588 KIH PGGL+ K I+ L + +A R + R A++ LSL + R VF++ Sbjct: 5281585 KIHKPGGLTEAQKSIVDHILMDKIKA-RKKVPRVRRHADKVH-----ALSLNYCRMVFNS 5281424 Query: 589 HKRMV 593 H+ +V Sbjct: 5281423 HRSLV 5281409 >emb|FN649745.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG20 Length = 1840930 Score = 32.7 bits (73), Expect = 4.9 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = -3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKG 122 VTL+D PGH I +I GA D +LV+ G Sbjct: 586916 VTLLDAPGHRDFIPKMISGASAADAAVLVVPAAVG 586812 >emb|FN649741.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG16 Length = 8064047 Score = 32.3 bits (72), Expect = 6.4 Identities = 16/37 (43%), Positives = 23/37 (62%) Frame = -3 Query: 114 MLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDL 150 +L++D T+G+Q Q+ Q A KLV V+ KIDL Sbjct: 5186802 LLLVDATQGVQAQTLSTAKAAQAAGLKLVPVVTKIDL 5186692 Database: E.siliculosus/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 195,934,119 Number of sequences in database: 35 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 125,793,127 Number of Sequences: 35 Number of extensions: 1896832 Number of successful extensions: 8249 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 7564 Number of HSP's gapped (non-prelim): 3040 length of query: 596 length of database: 65,311,373 effective HSP length: 119 effective length of query: 477 effective length of database: 65,307,208 effective search space: 31151538216 effective search space used: 31151538216 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 70 (31.6 bits)