TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor (eEFSec) # Homo sapiens # Complete (596 letters) Database: C.owczarzaki/genome.fa 89 sequences; 28,043,798 total letters Searching........................................................................................done Score E Sequences producing significant alignments: (bits) Value supercontig_1.5 of Capsaspora owczarzaki ATCC 30864 177 2e-78 supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 110 3e-24 supercontig_1.16 of Capsaspora owczarzaki ATCC 30864 61 2e-09 supercontig_1.17 of Capsaspora owczarzaki ATCC 30864 48 2e-05 supercontig_1.3 of Capsaspora owczarzaki ATCC 30864 42 0.001 supercontig_1.1 of Capsaspora owczarzaki ATCC 30864 42 0.001 supercontig_1.7 of Capsaspora owczarzaki ATCC 30864 39 0.010 supercontig_1.6 of Capsaspora owczarzaki ATCC 30864 39 0.010 supercontig_1.11 of Capsaspora owczarzaki ATCC 30864 38 0.017 supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 35 0.14 supercontig_1.15 of Capsaspora owczarzaki ATCC 30864 34 0.32 supercontig_1.8 of Capsaspora owczarzaki ATCC 30864 33 0.42 supercontig_1.9 of Capsaspora owczarzaki ATCC 30864 31 2.1 >supercontig_1.5 of Capsaspora owczarzaki ATCC 30864 Length = 1755934 Score = 177 bits (449), Expect(3) = 2e-78 Identities = 139/471 (29%), Positives = 208/471 (44%), Gaps = 130/471 (27%) Frame = -3 Query: 255 SVEIPALKVVKKVKSMQMFHMPIT------------------------------------ 278 ++EIP K+ +K+KS+QMFH P+ Sbjct: 66953 NIEIPTAKLSRKIKSIQMFHRPVQQITAVKEASDSKGRS*TFGLFFFFCFFVFVSCNQKA 66774 Query: 279 ---SAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKF 335 + QGDR GICVTQFD LERG CA ++ HA + V+K+ +F+ P+ +K++F Sbjct: 66773 DLLAKTQGDRAGICVTQFDASTLERGYACAAGAVIFAHALIARVDKVRFFKLPVLSKSRF 66594 Query: 336 HITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLT---------- 385 H + G + + Q P + + + + K ++ Sbjct: 66593 HGRRASLKLTGGVCGLI*SSGAPAQCPWVTKL*WERPHSLHAVSKQKSVSGEAAWPF*PF 66414 Query: 386 -----PAVTDND-----EADKKAGQATEGHCPRQQ--------------WALVEFEKPVT 421 A D + E DK+ A E P Q+ +AL+E + PV Sbjct: 66413 YSCVVRASLDGEGNARFEVDKEYAFAEELGRPPQENASNSGQPPAPLATFALIELDHPVA 66234 Query: 422 CPRLCLVIGSR--LDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLV 479 C L+IGSR LD D+HT CRLAF G + L D++Y ++FLP+LKV+K K + G V Sbjct: 66233 CLSNALLIGSRLDLDPDVHTKACRLAFSGRVHEALTDKSYRETFLPKLKVFKRKSRTGTV 66054 Query: 480 ERA----------------------------------MDDYSVIGRSLFKKETNIQLFVG 505 ER +DDY++IG+ LFKKET ++ F+G Sbjct: 66053 ERVRAPRVLVLPMGVFPHSQFAVLMPVLFCATTQTQILDDYTLIGKGLFKKETTLEPFLG 65874 Query: 506 LKVHLST---------------------GELGIIDSAFGQSGKFKIHIPGGLSPESKKIL 544 L+V LS+ GE+G I+ AFGQSGKF++ + +S +K L Sbjct: 65873 LQVGLSSGKQVELETSVACRSDSKASPAGEVGEIEGAFGQSGKFRVRMRQAVSDATKATL 65694 Query: 545 TPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQS 595 + K + G+G+A E A + + L+FKRYV+D KRM+QS Sbjct: 65693 AASDKPK---GKGKAPAPEAEA--------IEIVLSFKRYVYDRQKRMIQS 65574 Score = 100 bits (250), Expect(3) = 2e-78 Identities = 65/160 (40%), Positives = 77/160 (48%), Gaps = 62/160 (38%) Frame = -3 Query: 87 QVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE----------------- 129 Q TLVDCPGHA LIRTIIGGAQIID+MMLV+DV KG+QTQ+AE Sbjct: 67781 QFTLVDCPGHADLIRTIIGGAQIIDMMMLVVDVNKGIQTQTAEVRTHNLSLSGFARMLLT 67602 Query: 130 -------------CLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDK-------------- 162 CLVIG+I C LVV LNKID +PE +R I+K Sbjct: 67601 LARNPPPSSRLAQCLVIGEIMCDILVVALNKIDQIPEDQRAQTIEKARLA*NERSSFLCS 67422 Query: 163 ------------------MTKKMQKTLENTKFRGAPIIPV 184 +T+ + KT TKF +IPV Sbjct: 67421 LLTWGGGFRAVPTTVWMQVTRGLAKTFATTKFGQVAVIPV 67302 Score = 55.5 bits (132), Expect(3) = 2e-78 Identities = 23/48 (47%), Positives = 33/48 (68%) Frame = -2 Query: 207 ELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGD 254 ++L + D + PF+ +VDHCF+IKGQGTV+TGT LSG I + + Sbjct: 67182 QVLAKSVPRQRHDSAAPFVFAVDHCFAIKGQGTVLTGTALSGQIQVNE 67039 Score = 42.0 bits (97), Expect = 0.001 Identities = 21/30 (70%), Positives = 23/30 (76%), Gaps = 1/30 (3%) Frame = -2 Query: 36 FDKQPQSRERGITLDLGFSCFSV-PLPARL 64 FDK PQS+ RGITLDLGFS SV P+P L Sbjct: 68016 FDKNPQSQARGITLDLGFSSLSVTPMPEHL 67927 >supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 Length = 2668053 Score = 110 bits (275), Expect = 3e-24 Identities = 79/226 (34%), Positives = 117/226 (51%), Gaps = 7/226 (3%) Frame = +2 Query: 91 VDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIG-QIACQKLVVVLNKID 149 VDCPGHA I+ +I GA +D +LV+ T G Q+ E L++ Q+ + LVV +NK D Sbjct: 2000582 VDCPGHADYIKNMITGAAQMDGAILVVAGTDGQMPQTREHLLLAKQVGVKALVVYINKAD 2000761 Query: 150 LLPEGKRQAAIDKMTKKMQKTLENTKFRG--APIIPVXXXXXXXXXXXXXXXXXIPELIE 207 + E K Q + +M +M++ L KF G PII I +L++ Sbjct: 2000762 AVAE-KDQLELVEM--EMREILNEYKFDGDNTPIIIGSALCALEDREPELGRQSILKLLD 2000932 Query: 208 LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPAL---KVV 264 + + I P+RD PFLMS++ FSI G+GTV TG + G ++ GD VEI + Sbjct: 2000933 AVDNFIPQPSRDLDKPFLMSIEDVFSIGGRGTVATGRVERGIVNKGDEVEIVGFGTTPIK 2001112 Query: 265 KKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERG-LVCAPESL 309 V ++MFH + GD LG + + L RG ++CAP +L Sbjct: 2001113 TTVTGLEMFHKQLERGEAGDNLGALLRGVKREDLRRGHMICAPGTL 2001250 >supercontig_1.16 of Capsaspora owczarzaki ATCC 30864 Length = 797998 Score = 60.8 bits (146), Expect = 2e-09 Identities = 67/292 (22%), Positives = 110/292 (37%), Gaps = 25/292 (8%) Frame = -3 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 DK RERGIT+D+ F VT++D PGH Sbjct: 232604 DKLKAERERGITIDIALWKFETS-------------------------KFYVTIIDAPGH 232500 Query: 97 ASLIRTIIGGAQIIDLMMLVI---------DVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 I+ +I G D +L++ ++K QT+ L+ + ++L+V +NK Sbjct: 232499 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKDGQTRE-HALLAYTLGVKQLIVGINK 232323 Query: 148 IDLLP--EGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIP-- 203 +D + E + + +++ ++K + K +P+ Sbjct: 232322 MDSIKFAEERYNEIVTEVSNYIKKIGYDPKT--VAFVPISGWHGDNMLEASENMPWFKGW 232149 Query: 204 ------------ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSIS 251 LIE L + IS P R P + + + I G GTV G + +G + Sbjct: 232148 TIERKEGNASGKTLIEALDA-ISPPKRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLK 231972 Query: 252 LGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLV 303 G V V +VKS++M H + A GD +G V K + RG V Sbjct: 231971 PGMVVTFAPSNVTTEVKSVEMHHESLPEANPGDNVGFNVKNVAVKDIRRGNV 231816 >supercontig_1.17 of Capsaspora owczarzaki ATCC 30864 Length = 792894 Score = 48.1 bits (113), Expect = 2e-05 Identities = 39/152 (25%), Positives = 62/152 (40%), Gaps = 9/152 (5%) Frame = +3 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 D P RE+G T+++G + F + T++D PGH Sbjct: 585975 DTNPDEREKGKTVEVGRATFGTS-------------------------KKRFTILDAPGH 586079 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKGM---------QTQSAECLVIGQIACQKLVVVLNK 147 S + +IGGA D+ +LVI KG QT+ +++ + LVVV+NK Sbjct: 586080 KSYVPNMIGGAAQADVAVLVISARKGEFETGFERGGQTRE-HAMLVKTAGVKHLVVVINK 586256 Query: 148 IDLLPEGKRQAAIDKMTKKMQKTLENTKFRGA 179 +D G Q D+ K+ L+ + A Sbjct: 586257 MDDPTVGWAQERYDECVSKLTPYLKQCGYNTA 586352 >supercontig_1.3 of Capsaspora owczarzaki ATCC 30864 Length = 2526540 Score = 42.0 bits (97), Expect = 0.001 Identities = 34/169 (20%), Positives = 68/169 (40%) Frame = +3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + +L++D +G+Q Q+ ++ + +V VLNK Sbjct: 102420 INLIDTPGHVDFSYEVSRSLYACQGCILLVDAAQGIQAQTVANFMLAFDSNLSIVPVLNK 102599 Query: 148 IDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPELIE 207 IDLL A ++TK+MQ I I +++ Sbjct: 102600 IDLL-----HADAARVTKQMQSVFGTEPEEALRI-------------SAKTNLGIDQVLP 102725 Query: 208 LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSV 256 + ++ P+ +P P + + + +G V ++ GS+ +G+ + Sbjct: 102726 AIIDRVPPPSGNPDAPPKVLLFDSWYDDFRGVVCLIAVVDGSLKVGEKI 102872 >supercontig_1.1 of Capsaspora owczarzaki ATCC 30864 Length = 3794667 Score = 42.0 bits (97), Expect = 0.001 Identities = 19/60 (31%), Positives = 34/60 (56%) Frame = -1 Query: 90 LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149 ++D PGH S G+ + D+ +LV+D+ G++ Q+ E + + + VV LNK+D Sbjct: 3383196 IIDTPGHESFSNLRSRGSSLCDIAILVVDIMHGLEPQTIESINLLKKMKTPFVVALNKVD 3383017 Score = 33.1 bits (74), Expect = 0.55 Identities = 16/62 (25%), Positives = 30/62 (48%) Frame = +1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + ++ D ++V+D G+ Q+ L K V+++NK Sbjct: 1294534 INLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNK 1294713 Query: 148 ID 149 +D Sbjct: 1294714 MD 1294719 Score = 29.3 bits (64), Expect = 7.9 Identities = 31/110 (28%), Positives = 46/110 (41%), Gaps = 2/110 (1%) Frame = +1 Query: 413 LVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILL--HGLEDRNYADSFLPRLKVY 470 LVE K V R LV+G L+A + A G L H L DR + + L Sbjct: 227989 LVEGHKLVNAERTQLVVGQALEAALQLGEVD-AREGNLQQPHKLGDRQSSRALRVALPKV 228165 Query: 471 KLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDS 520 + HGL +RA+ +V+G + + V L G +G +D+ Sbjct: 228166 ADERVHGLADRALRS-NVLGLGFLGERFRVAAVVHGAGRLRNGCVGAVDA 228312 >supercontig_1.7 of Capsaspora owczarzaki ATCC 30864 Length = 1544042 Score = 38.9 bits (89), Expect = 0.010 Identities = 32/126 (25%), Positives = 58/126 (46%), Gaps = 8/126 (6%) Frame = -3 Query: 141 LVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXX 200 ++++ NKIDL+ R+A + +++ K ++ T +GAPIIP+ Sbjct: 697167 IIILQNKIDLV----REAQALEQHQEILKFVDGTVAQGAPIIPI----------SAQLKY 697030 Query: 201 XIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKG--------QGTVMTGTILSGSISL 252 I + E + ++I IP RD + + V F + +G V G+IL G + Sbjct: 697029 NIDCVCEYIANKIPIPVRDFTAAPRLIVIRSFDVNKPGAEVDDLKGGVAGGSILVGVLKA 696850 Query: 253 GDSVEI 258 G +E+ Sbjct: 696849 GQEIEV 696832 >supercontig_1.6 of Capsaspora owczarzaki ATCC 30864 Length = 1617845 Score = 38.9 bits (89), Expect = 0.010 Identities = 22/72 (30%), Positives = 38/72 (52%), Gaps = 9/72 (12%) Frame = -2 Query: 87 QVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDV---------TKGMQTQSAECLVIGQIA 137 ++TL+D PGH I +I GA D+ +LV++ G QT+ L++ + Sbjct: 1409206 RITLLDAPGHRDFIPNMISGAAQADVAVLVVNAGVGEFEAGFEGGGQTRE-HALLVRSLG 1409030 Query: 138 CQKLVVVLNKID 149 +L+V +NK+D Sbjct: 1409029 VNQLIVAVNKLD 1408994 Score = 35.0 bits (79), Expect = 0.14 Identities = 18/51 (35%), Positives = 25/51 (49%) Frame = -1 Query: 208 LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI 258 L + Q P R P SV+ FS G + G ++SGS+ +GD V I Sbjct: 1408613 LASDQFQPPERPIDKPLRFSVNDIFSRPNSGVSLGGKVISGSVQIGDKVLI 1408461 >supercontig_1.11 of Capsaspora owczarzaki ATCC 30864 Length = 1292811 Score = 38.1 bits (87), Expect = 0.017 Identities = 30/122 (24%), Positives = 49/122 (40%), Gaps = 9/122 (7%) Frame = +3 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 DK RERGIT+D+ F VT++D PGH Sbjct: 611094 DKLKAERERGITIDIALWKFETS-------------------------KFYVTIIDAPGH 611198 Query: 97 ASLIRTIIGGAQIIDLMMLVI---------DVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 I+ +I G D +L++ ++K QT+ L+ + ++L+V +NK Sbjct: 611199 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKDGQTRE-HALLAYTLGVKQLIVGINK 611375 Query: 148 ID 149 +D Sbjct: 611376 MD 611381 >supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 Length = 955311 Score = 35.0 bits (79), Expect = 0.14 Identities = 19/64 (29%), Positives = 34/64 (53%) Frame = +1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + ++ D ++++DV +G+ Q+ L + K V+VLNK Sbjct: 236941 LNLIDSPGHVDFSSEVSTAVRLSDGAVVLVDVVEGVCPQTHAVLRQAWLEGIKPVLVLNK 237120 Query: 148 IDLL 151 +D L Sbjct: 237121 MDRL 237132 >supercontig_1.15 of Capsaspora owczarzaki ATCC 30864 Length = 930169 Score = 33.9 bits (76), Expect = 0.32 Identities = 17/62 (27%), Positives = 30/62 (48%) Frame = +3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + ++ D ++V+D G+ Q+ L K V++LNK Sbjct: 67080 INLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAITERIKPVLMLNK 67259 Query: 148 ID 149 +D Sbjct: 67260 MD 67265 >supercontig_1.8 of Capsaspora owczarzaki ATCC 30864 Length = 1512001 Score = 33.5 bits (75), Expect = 0.42 Identities = 18/84 (21%), Positives = 39/84 (46%), Gaps = 4/84 (4%) Frame = -1 Query: 231 CFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVK----KVKSMQMFHMPITSAMQGDRL 286 C KG GTV++GT+++G I D++ + + ++K++Q +P+ G Sbjct: 378199 CCHAKGVGTVISGTVMAGVIRSTDTLYLGPDTLGNFQPVQLKTIQRKRLPVRDVRAGQAA 378020 Query: 287 GICVTQFDPKLLERGLVCAPESLH 310 + + + +G+V + H Sbjct: 378019 SFALKKIKRSAIRKGMVLLAKDAH 377948 Score = 32.3 bits (72), Expect = 0.94 Identities = 12/44 (27%), Positives = 25/44 (56%) Frame = -2 Query: 90 LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVI 133 L+D PGHA+ + ++ D ++LV+D +G++ C ++ Sbjct: 1427514 LIDTPGHANFYDEAVAAMRLADGVLLVVDAVEGVRDTGRFCWLL 1427383 >supercontig_1.9 of Capsaspora owczarzaki ATCC 30864 Length = 1380613 Score = 31.2 bits (69), Expect = 2.1 Identities = 22/74 (29%), Positives = 31/74 (41%) Frame = -3 Query: 203 PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALK 262 PE + +S S+ T D + +V C +G+ T SGS S +S PAL Sbjct: 605882 PEDLPFYSSAASVETPDST-----TVSACTPSPAEGSANTSLASSGSASASESTAPPALA 605718 Query: 263 VVKKVKSMQMFHMP 276 + V S MP Sbjct: 605717 DAQTVSSDDSMEMP 605676 Database: C.owczarzaki/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 28,043,798 Number of sequences in database: 89 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,358,665 Number of Sequences: 89 Number of extensions: 255515 Number of successful extensions: 1304 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 916 Number of HSP's gapped (non-prelim): 742 length of query: 596 length of database: 9,347,932 effective HSP length: 105 effective length of query: 491 effective length of database: 9,338,587 effective search space: 4585246217 effective search space used: 4585246217 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 63 (28.9 bits)