TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor (eEFSec) # Homo sapiens # Complete (596 letters) Database: C.muris/genome.fa 84 sequences; 9,245,251 total letters Searching...................................................................................done Score E Sequences producing significant alignments: (bits) Value gb|DS989728.1| Cryptosporidium muris RN66 scf_1106632373407 geno... 72 5e-13 gb|DS989726.1| Cryptosporidium muris RN66 scf_1106632373453 geno... 67 9e-12 gb|DS989733.1| Cryptosporidium muris RN66 scf_1106632373425 geno... 47 9e-06 gb|DS989729.1| Cryptosporidium muris RN66 scf_1106632373431 geno... 40 0.001 gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 geno... 35 0.063 gb|DS989736.1| Cryptosporidium muris RN66 scf_1106632373433 geno... 32 0.53 gb|DS989732.1| Cryptosporidium muris RN66 scf_1106632373461 geno... 29 3.5 gb|DS989734.1| Cryptosporidium muris RN66 scf_1106632354001 geno... 28 5.9 >gb|DS989728.1| Cryptosporidium muris RN66 scf_1106632373407 genomic scaffold, whole genome shotgun sequence Length = 965821 Score = 71.6 bits (174), Expect = 5e-13 Identities = 65/280 (23%), Positives = 114/280 (40%), Gaps = 11/280 (3%) Frame = +2 Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGH 96 DK RERGIT+D+ F P + T++D PGH Sbjct: 137837 DKLKAERERGITIDIALWKFETP-------------------------RYEYTVIDAPGH 137941 Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE------CLVIGQIACQKLVVVLNKIDL 150 I+ +I G D+ +LV+ + S E L+ + ++++V +NK+D Sbjct: 137942 RDFIKNMITGTSQADVALLVVPADRFEGAFSKEGQTREHALLAFTLGVKQMIVGINKMDT 138121 Query: 151 LPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIP-----EL 205 +Q+ D++ +++ L+ + IP +P L Sbjct: 138122 CDY--KQSRYDEIHNEVEGYLKKVGYN-IEKIPFVAISGFVGDNMVEKSDKMPWYKGRTL 138292 Query: 206 IELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVK 265 +E L + + P R P + + + I G GTV G + +G I G +V + + Sbjct: 138293 VEALDT-MEPPKRPTEKPLRLPLQDVYKIGGVGTVPVGRVETGIIKPGMNVTFAPVGITT 138469 Query: 266 KVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305 +VKS++M H ++ A GD +G V K ++RG V + Sbjct: 138470 EVKSVEMHHEQLSEAGPGDNVGFNVKNVSIKDIKRGYVAS 138589 Score = 41.2 bits (95), Expect = 7e-04 Identities = 18/64 (28%), Positives = 36/64 (56%) Frame = -1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + ++ D ++++DV +G+ Q+ + L I ++++VLNK Sbjct: 143782 INLIDSPGHVDFAHEVSSALRLCDGALILVDVVEGLGDQTRKVLQQAYIERVQMLLVLNK 143603 Query: 148 IDLL 151 +D L Sbjct: 143602 MDRL 143591 Score = 29.3 bits (64), Expect = 2.6 Identities = 32/140 (22%), Positives = 59/140 (42%), Gaps = 15/140 (10%) Frame = +1 Query: 233 SIKGQGTVMTGTILSGSISLGDS--VEIPALKVVKKVKSMQMFHMPITSAMQGDRL--GI 288 S+ G T++ L+ +ISL S V I A + + + + + +P + ++ I Sbjct: 21217 SVNGNSTILAELSLTMNISLSSSTPVYIAAYALCQNIPTQPLAVIPSQVLLSEIQMYGSI 21396 Query: 289 CVTQFDPKL---LERGLVCAPE---SLHTVHAALISV-----EKIPYFRGPLQTKAKFHI 337 +T + P L L + C S + V+++ I E +PY +++ Sbjct: 21397 IITTYIPPLGETLRTSIDCYINIFASFNQVYSSKIGTFSSFGEGVPYTPPNGVAFYSYYV 21576 Query: 338 TVGHETVMGRLMFFSPAPDN 357 +V +MG L F P P N Sbjct: 21577 SVDAPPIMGSLTLFEPNPGN 21636 >gb|DS989726.1| Cryptosporidium muris RN66 scf_1106632373453 genomic scaffold, whole genome shotgun sequence Length = 1324930 Score = 67.4 bits (163), Expect = 9e-12 Identities = 62/233 (26%), Positives = 111/233 (47%), Gaps = 23/233 (9%) Frame = -1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL- 145 V+ VDCPGH L+ T++ GA ++D +L++ + Q Q++E L +I + +++L Sbjct: 1321795 VSFVDCPGHDILMSTMLNGAAVMDAALLLVAGNESCPQPQTSEHLAAVEIMKLRHIIILQ 1321616 Query: 146 NKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPEL 205 NK++L+ E + AI++ K+++ + T + A IIP+ I L Sbjct: 1321615 NKVELIKEAQ---AIEQ-HKQIKDFVAGTSAQDAAIIPI----------SAVLKFNIDVL 1321478 Query: 206 IELLTSQISIPTRDPSGPFLMSVDHCFSIKG--------QGTVMTGTILSGSISLGDSVE 257 E + +QI IP RD + M V F + +G V G+I+ G + LGD +E Sbjct: 1321477 CEYICTQIPIPIRDFTSSPRMIVIRSFDVNKPGEDAKSMRGGVAGGSIIRGVLKLGDEIE 1321298 Query: 258 I-PAL------------KVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKL 297 + P + + ++ S+ H + A+ G +G+ T+ DP L Sbjct: 1321297 VRPGIYNKNSEGAFVCRPIRSRIVSLFAEHNDLQYAVSGGLIGV-GTKIDPTL 1321142 >gb|DS989733.1| Cryptosporidium muris RN66 scf_1106632373425 genomic scaffold, whole genome shotgun sequence Length = 528544 Score = 47.4 bits (111), Expect = 9e-06 Identities = 55/256 (21%), Positives = 98/256 (38%), Gaps = 35/256 (13%) Frame = -1 Query: 89 TLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM---------QTQSAECLVIGQIACQ 139 T++D PGH S + +I GA D+ +L+I KG QT+ ++ + Sbjct: 390982 TILDAPGHKSFVPNMISGASQADIGVLIISARKGEFETGFERGGQTRE-HAMLAKTLGVN 390806 Query: 140 KLVVVLNKID---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXX 196 +L+V +NK+D + R I K K + +P+ Sbjct: 390805 QLIVAINKMDDPTCQWDETRYKDIQKKLTPYLKICGYNPNKDIFFVPISGQLGQNLKYHV 390626 Query: 197 XXXXXI--------------PELIELLTSQISIPTRD------PSGPFLMSVDHCFSIKG 236 P L +++ IS+P R P P ++ + + Sbjct: 390625 SDKSNTKYYEPKAFWYSTDQPTLFDIMDKIISVPDRGINSSGIPIDPEVVRIPLLDGYRD 390446 Query: 237 QGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFH--MPITSAMQGDRLGICVTQFD 294 G + G I G I+ GDS+ + KV K++S+ + + + G+ + + + D Sbjct: 390445 NGVIAMGKIELGIINCGDSLVVMPNKVKVKIQSIWLGEDTEEYSWSGPGENVRLKLVNID 390266 Query: 295 PKLLERGLV-CAPESL 309 L +G V C+P SL Sbjct: 390265 EDSLNKGFVLCSPSSL 390218 Score = 29.3 bits (64), Expect = 2.6 Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 5/51 (9%) Frame = -3 Query: 108 QIIDLMMLVIDVTKGMQTQSAECLVIGQIACQK-----LVVVLNKIDLLPE 153 ++ID ++I V Q C+ I + ++ LV VLNK+DL+P+ Sbjct: 419405 KVIDSSDIIIHVLDSRDPQGTRCIYIEEYLEKECPQKYLVYVLNKVDLIPK 419253 >gb|DS989729.1| Cryptosporidium muris RN66 scf_1106632373431 genomic scaffold, whole genome shotgun sequence Length = 772423 Score = 40.4 bits (93), Expect = 0.001 Identities = 19/61 (31%), Positives = 34/61 (55%) Frame = -2 Query: 91 VDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDL 150 +D PGH S G+ + D+ +LV+D+ G++ Q+ E + + + ++ LNKID Sbjct: 152274 IDTPGHESFNNLRSRGSSLCDIAVLVVDIMHGLEPQTKESIGLLRNRKCPFIIALNKIDR 152095 Query: 151 L 151 L Sbjct: 152094 L 152092 >gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 genomic scaffold, whole genome shotgun sequence Length = 1306717 Score = 34.7 bits (78), Expect = 0.063 Identities = 22/70 (31%), Positives = 35/70 (50%), Gaps = 7/70 (10%) Frame = -3 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVID-------VTKGMQTQSAECLVIGQIACQK 140 VT+ D PGH I + + + +LVID TKG+ + ++ G + Sbjct: 566585 VTIFDTPGHKDFIPSTYAATILAESAILVIDCDNFNQRFTKGLIKEHLLFIISGNLL--N 566412 Query: 141 LVVVLNKIDL 150 L+VV+NK+DL Sbjct: 566411 LIVVINKMDL 566382 Score = 30.0 bits (66), Expect = 1.6 Identities = 15/62 (24%), Positives = 29/62 (46%) Frame = -1 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + L+D PGH + ++ D ++V+D G+ Q+ L + V+ +NK Sbjct: 1048831 INLIDSPGHVDFSSEVTAALRVTDGALVVVDAVDGVCIQTETVLRQALNERIRPVLHVNK 1048652 Query: 148 ID 149 +D Sbjct: 1048651 VD 1048646 >gb|DS989736.1| Cryptosporidium muris RN66 scf_1106632373433 genomic scaffold, whole genome shotgun sequence Length = 367275 Score = 31.6 bits (70), Expect = 0.53 Identities = 14/64 (21%), Positives = 31/64 (48%) Frame = +2 Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147 + ++D PGH + + ++ D ++V+D G+ + + +V+++NK Sbjct: 272870 LNIIDTPGHVNFMDEFCASIRLCDGAVIVVDAILGISKHTERMIYYCLREKHDIVLLINK 273049 Query: 148 IDLL 151 ID L Sbjct: 273050 IDRL 273061 >gb|DS989732.1| Cryptosporidium muris RN66 scf_1106632373461 genomic scaffold, whole genome shotgun sequence Length = 567910 Score = 28.9 bits (63), Expect = 3.5 Identities = 13/47 (27%), Positives = 30/47 (63%), Gaps = 2/47 (4%) Frame = +2 Query: 108 QIIDLMMLVIDVTK--GMQTQSAECLVIGQIACQKLVVVLNKIDLLP 152 ++ D+++ ++D G + E ++GQ +KL++++NK+DL+P Sbjct: 465047 EVSDIILEILDARDPLGYRCHDLERSIVGQ--GKKLILIINKVDLVP 465181 >gb|DS989734.1| Cryptosporidium muris RN66 scf_1106632354001 genomic scaffold, whole genome shotgun sequence Length = 406819 Score = 28.1 bits (61), Expect = 5.9 Identities = 27/97 (27%), Positives = 43/97 (44%), Gaps = 2/97 (2%) Frame = -1 Query: 292 QFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMG--RLM 349 +FDPK++ L E LH+ L ++ IP + T A + + H + ++M Sbjct: 117829 EFDPKIILEELCNITEELHSTRDLLEDLDDIPL---EMPTSAPISL-LNHPAYVAILKIM 117662 Query: 350 FFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTP 386 F DNF I + E LF+ Q + K+L P Sbjct: 117661 GFKEPEDNFSLWSIPKQVS-QDELLFKLQKI-KELVP 117557 Database: C.muris/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 9,245,251 Number of sequences in database: 84 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,346,048 Number of Sequences: 84 Number of extensions: 53070 Number of successful extensions: 184 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 152 Number of HSP's gapped (non-prelim): 94 length of query: 596 length of database: 3,081,750 effective HSP length: 97 effective length of query: 499 effective length of database: 3,073,602 effective search space: 1533727398 effective search space used: 1533727398 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 59 (27.3 bits)