TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium transferase [Homo sapiens] (501 letters) Database: P.ultimum/genome.fa 82 sequences; 42,801,618 total letters Searching.................................................................................done Score E Sequences producing significant alignments: (bits) Value gb|GL376621.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 394 e-120 gb|GL376571.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 33 0.51 gb|GL376634.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 32 1.5 gb|GL376603.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 32 2.0 gb|GL376573.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 32 2.0 gb|GL376628.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 30 4.4 gb|GL376567.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 30 5.7 gb|GL376608.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 30 7.4 >gb|GL376621.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582024, whole genome shotgun sequence Length = 492436 Score = 394 bits (1013), Expect(2) = e-120 Identities = 197/409 (48%), Positives = 269/409 (65%), Gaps = 36/409 (8%) Frame = -2 Query: 1 MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAI 60 MN ++ E V+P YV QG A R+ E L LL + + P++GWDE T+EL L LA Sbjct: 366585 MNAKNAQLAEAFVAPTYVEQGLAAIRNREQLFTALLSQRRVPDDGWDEQTVELVLQSLAA 366406 Query: 61 MDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNS 120 MDSNNF N G GERE RV S+LVA+RH+ HG+GRSGD++AVQPKAAGSSL+ ++TN+ Sbjct: 366405 MDSNNFRTNIGAGEREARVFSSLVAKRHFHLCHGVGRSGDVAAVQPKAAGSSLIVQLTNA 366226 Query: 121 LVLDIIKLAGVHTVANCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMI 180 L D+++ AG+ ++ + V+P+ATGMSLTL LTLR ++P AKY+IWPRIDQKSCFKS++ Sbjct: 366225 LAKDLLREAGLRSITSALVLPVATGMSLTLVLLTLRAQKPDAKYVIWPRIDQKSCFKSIL 366046 Query: 181 TAGFEPVVIENVL--EGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRLEEL 238 TAG P+V+EN L +GD+LRTD+ ++ + +L + STTSCFAPR D +E + Sbjct: 366045 TAGLTPLVLENTLSEDGDQLRTDMDGLQRLIDAHRSGDVLAVLSTTSCFAPRGYDDVEAI 365866 Query: 239 AVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGF 298 A +C +DI H++NNAYGVQ+SKC H++ Q RVGR+DA VQSLDKNFMVPVGGAI+ Sbjct: 365865 ARVCQTHDIAHVINNAYGVQASKCTHVVNQAMRVGRVDAIVQSLDKNFMVPVGGAIVCAP 365686 Query: 299 NDSFIQEISKMYPGRASASPSLDVLITLLSL----------------------------- 329 + + +SK YPGRA+ +P+LD IT+L + Sbjct: 365685 SAEMVASVSKCYPGRAAITPTLDFFITMLQMVL*PLAMSTCLRFSDTCASRADTLLLLLL 365506 Query: 330 -----GSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISL 373 G GY++LL+ERK + YL Q++ ++ ER+LH P N + L Sbjct: 365505 FS*R*GRKGYRQLLQERKTLAKYLQEQLQIVAHEVGERVLHVPFNEVLL 365359 Score = 57.4 bits (137), Expect(2) = e-120 Identities = 40/144 (27%), Positives = 62/144 (43%), Gaps = 40/144 (27%) Frame = -1 Query: 371 ISLAMTLKTLDEHRD-----KAVTQLGSMLFTRQVSGAR--------------------- 404 IS AMTL T D + +T G+MLF+R VSG R Sbjct: 365275 ISFAMTLSTFSTASDARTKAQELTFFGAMLFSRGVSGTRYVSATPCAIPSVRSHGILCVC 365096 Query: 405 --------------VVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLF 450 VV +T+ GYTF + +H++++P YL A AIGM +VD Sbjct: 365095 VCMCMCFAKIQTNSVVSCLDNKTIGGYTFESYGAHSDHFPAPYLTVACAIGMTKDEVDAL 364916 Query: 451 IKRLDRCLKAVRKERSKESDDNYD 474 + +L + + R + ++ S ++ D Sbjct: 364915 VVKLRKTIHEWRTKHNRSSSNSVD 364844 >gb|GL376571.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581381, whole genome shotgun sequence Length = 502691 Score = 33.5 bits (75), Expect = 0.51 Identities = 21/59 (35%), Positives = 30/59 (50%) Frame = -2 Query: 443 KMQDVDLFIKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDASS 501 K ++ K+ + LKA +KE+ +SDD D+ E D EE DN LD + A S Sbjct: 152293 KEKEAAKLAKKEKKALKAAKKEKKTKSDDKEDEDESGDDEE----ADNEDLDASEKADS 152129 >gb|GL376634.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582037, whole genome shotgun sequence Length = 1414051 Score = 32.0 bits (71), Expect = 1.5 Identities = 29/103 (28%), Positives = 40/103 (38%), Gaps = 13/103 (12%) Frame = -2 Query: 1 MNRESFAAGERLVSPAYVRQGCEARR-SHEHLIRLLLEKGKCPENGWDESTLELFLHELA 59 M+ ++A L Y Q E R SH +++ + GKC E GW ES ++ Sbjct: 897066 MHNAAYAVTISLYFSLYRHQPIETRA*SHLRVVKEAQQAGKCNERGWSESKRRGLPQKIQ 896887 Query: 60 IMDSNNFLGNCGVGEREGR------------VASALVARRHYR 90 G REG VA+AL+ RRH R Sbjct: 896886 ------------AG*REGMHNKA*A*TLGLVVAAALLVRRHLR 896794 >gb|GL376603.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582006, whole genome shotgun sequence Length = 1270039 Score = 31.6 bits (70), Expect = 2.0 Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 7/69 (10%) Frame = +3 Query: 160 PKAKYIIWPRIDQKSCFKSM------ITAGFEPVVIENVLEG-DELRTDLKAVEAKVQEL 212 PKA Y +WPR+ S S+ +T G P V+ N + G + R+ A V + Sbjct: 243330 PKALYNVWPRLRAVSLLHSLAFWLLWLTVGLMPFVVVNAIAGKGQQRSGRVDPSAHVPKY 243509 Query: 213 GPDCILCIH 221 G L +H Sbjct: 243510 GKQRTLSMH 243536 >gb|GL376573.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581402, whole genome shotgun sequence Length = 979788 Score = 31.6 bits (70), Expect = 2.0 Identities = 23/72 (31%), Positives = 39/72 (54%), Gaps = 4/72 (5%) Frame = -1 Query: 119 NSLVLDIIKLAG--VHTVANCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRI--DQKS 174 N+ VL + +G + + A VVP+A+ +T T RH++ A++I++ RI D K+ Sbjct: 567405 NASVLSHLPKSGSALASAAEAVVVPIASIFDVTSIIATFRHQK-IARFILFDRIPTDTKA 567229 Query: 175 CFKSMITAGFEP 186 C S+ F P Sbjct: 567228 CPTSLSWCTFNP 567193 >gb|GL376628.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582031, whole genome shotgun sequence Length = 1316296 Score = 30.4 bits (67), Expect = 4.4 Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 3/68 (4%) Frame = -2 Query: 117 ITNSLVLDIIKLAGVHTVANCFVVPMATGMSLTLCFLTLR---HKRPKAKYIIWPRIDQK 173 + SL D +++ VH NCF + + + LCF +LR H R +K + Sbjct: 817473 VQGSLASDTVQVRYVHLALNCFPLSLCNVPHVALCFGSLRWTGHLRFHSKQRARQVEED* 817294 Query: 174 SCFKSMIT 181 CF++ ++ Sbjct: 817293 KCFRTRVS 817270 >gb|GL376567.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581354, whole genome shotgun sequence Length = 1199448 Score = 30.0 bits (66), Expect = 5.7 Identities = 18/65 (27%), Positives = 33/65 (50%) Frame = -2 Query: 336 KLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSML 395 K L+ + E + L N +KK +E +E + N + L ++ EH+++A+ +L Sbjct: 882572 KHLESQMEEYESLLNTVKKNAERKDEIFSNRYQNVCARLRELNSIVEHKERAIDELEEKN 882393 Query: 396 FTRQV 400 T QV Sbjct: 882392 RTLQV 882378 >gb|GL376608.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582011, whole genome shotgun sequence Length = 418791 Score = 29.6 bits (65), Expect = 7.4 Identities = 13/20 (65%), Positives = 14/20 (70%) Frame = +2 Query: 411 MQTVSGYTFRGFMSHTNNYP 430 +Q VSGYTF F HTNN P Sbjct: 170378 IQFVSGYTFLFFGGHTNNIP 170437 Database: P.ultimum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 42,801,618 Number of sequences in database: 82 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 24,438,253 Number of Sequences: 82 Number of extensions: 371643 Number of successful extensions: 2244 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 2137 Number of HSP's gapped (non-prelim): 389 length of query: 501 length of database: 14,267,206 effective HSP length: 107 effective length of query: 394 effective length of database: 14,258,432 effective search space: 5617822208 effective search space used: 5617822208 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 64 (29.3 bits)