TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium transferase [Homo sapiens] (501 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 233 2e-61 gb|CM000621.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 30 4.2 gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 29 5.4 >gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 5, whole genome shotgun sequence Length = 1098047 Score = 233 bits (594), Expect = 2e-61 Identities = 130/293 (44%), Positives = 182/293 (62%), Gaps = 28/293 (9%) Frame = -1 Query: 27 SHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGN------------CGVGE 74 SH+ L R LL + PE+GW + ++ L+ +A +D+NN CGVGE Sbjct: 920582 SHKDL-RTLLVNRRLPEHGWSDVQIQHVLYTMATLDTNNKRSGSSSSDDSGSSRWCGVGE 920406 Query: 75 REGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKL-AGVH- 132 REGRV SALV+ RH+ HG+GRSGDI+ QPKA GS++L K+T LVLD ++ +G+H Sbjct: 920405 REGRVYSALVSSRHFGLSHGVGRSGDIAEPQPKALGSTVLAKLTLCLVLDAVRRGSGLHP 920226 Query: 133 --TVANCFVVPMATGMSLTLCFLTLRHK------------RPKAKYIIWPRIDQKSCFKS 178 + ++P+ TGMS+ L TLR +P ++W RIDQKSCFK+ Sbjct: 920225 QTAAKSGILLPLCTGMSMALVLGTLRQLQDNHQTSANAAIKPYRNIVLWSRIDQKSCFKA 920046 Query: 179 MITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRLEEL 238 + +AG E VV+ L GD + TDL A +A + G D +L + +TTSCFAPRVPD+++ + Sbjct: 920045 IQSAGLECVVVPTRLAGDAVVTDLDAFQASLLTYG-DRVLAVVTTTSCFAPRVPDQVDAV 919869 Query: 239 AVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVG 291 A +CA +PH++NNAYG+QSS L+ + VGR+DA V S DKNFMVP+G Sbjct: 919868 ARLCAEQCVPHVINNAYGLQSSDTSKLLNRACAVGRVDAIVCSTDKNFMVPIG 919710 Score = 134 bits (337), Expect = 1e-31 Identities = 79/193 (40%), Positives = 113/193 (58%), Gaps = 13/193 (6%) Frame = -3 Query: 290 VGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLS 349 +GGAI+ ++S IQ + K+Y GRASASP LD+ ITL S+G GY++LL R+ + + Sbjct: 919584 IGGAIVLSPDESLIQNVGKVYAGRASASPMLDLFITLCSMGLRGYQQLLTSRRILLPTFT 919405 Query: 350 NQIKKLSEAYNERLLHTPHNPISLAMTLKTL------DEHR-------DKAVTQLGSMLF 396 ++K+++ Y ER+L N IS +TL L DE + V+ LG+MLF Sbjct: 919404 ERLKEVANKYGERVLECRTNCISFGVTLDGLVRPPRQDEDDVAYRTSVSRDVSSLGAMLF 919225 Query: 397 TRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDR 456 R VSG RVVP G++Q + G F GF S + YP AYL AA AIG+ + D F RLD+ Sbjct: 919224 QRCVSGTRVVPRGTVQRMGGMEFCGFGSSHDAYPHAYLTAACAIGLTASEGDDFFTRLDQ 919045 Query: 457 CLKAVRKERSKES 469 L +K + + + Sbjct: 919044 TLHEFQKRQRRRA 919006 Score = 84.3 bits (207), Expect = 1e-16 Identities = 44/100 (44%), Positives = 66/100 (66%), Gaps = 1/100 (1%) Frame = -3 Query: 159 RPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCIL 218 +P ++W IDQKSCFK++ +AG E VV+ LEGD + TDL A +A + G D +L Sbjct: 914583 KPSRNIVLWSWIDQKSCFKAIQSAGLECVVVPTRLEGDAVVTDLDAFQAFLLAYG-DRVL 914407 Query: 219 CIHSTTSCFAPRVPDRLEELAVICANYDIP-HIVNNAYGV 257 + +TTSCFAP+VPD+++ +A +CA +P VN ++ V Sbjct: 914406 AVVTTTSCFAPQVPDQVDAVARLCAEQ*LPTSTVNRSWSV 914287 Score = 67.8 bits (164), Expect = 1e-11 Identities = 36/82 (43%), Positives = 50/82 (60%) Frame = -1 Query: 388 VTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDV 447 V+ G+MLF R VSG R+VP G++Q + G F GF S + YP AYL AA AIG+ + Sbjct: 914216 VSSSGAMLFQRCVSGTRIVPRGTVQRMGGMEFCGFGSSHDAYPHAYLTAACAIGLTASEG 914037 Query: 448 DLFIKRLDRCLKAVRKERSKES 469 D F RLD+ L +K + + + Sbjct: 914036 DDFFTRLDQTLHEFQKRQRRRA 913971 >gb|CM000621.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 19, whole genome shotgun sequence Length = 690427 Score = 29.6 bits (65), Expect = 4.2 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 5/62 (8%) Frame = +2 Query: 299 NDSFIQEISKMYPGRASASPSL-----DVLITLLSLGSNGYKKLLKERKEMFSYLSNQIK 353 ND +++ S P R S SP + D++ TLL + SN Y + R + + + + K Sbjct: 121286 NDPLLKKRSPCGPRRTSVSP*IESVRWDIVTTLLRIHSNAYSESSSNRIYLSTSFTYRCK 121465 Query: 354 KL 355 KL Sbjct: 121466 KL 121471 >gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 27, whole genome shotgun sequence Length = 404298 Score = 29.3 bits (64), Expect = 5.4 Identities = 24/113 (21%), Positives = 53/113 (46%), Gaps = 5/113 (4%) Frame = -2 Query: 276 DAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYK 335 DAF Q ++ ++PVG ++ G + ++ GR++A+P LD + ++ K Sbjct: 74585 DAFDQYINAEVLLPVGDTLLTGKVIARKKDRDGNPVGRSNANPILDSRVLMVQFNDGVVK 74406 Query: 336 KLLKE--RKEMFSYLSNQIKK---LSEAYNERLLHTPHNPISLAMTLKTLDEH 383 + + ++S + N+ ++ L E + R + P L +T + ++H Sbjct: 74405 EFAANVIAEHIYSQVDNEGRRHLILDEIVDHRKNGSAVAPDDLYVTDRNGNQH 74247 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,150,411 Number of Sequences: 31 Number of extensions: 194237 Number of successful extensions: 1014 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 974 Number of HSP's gapped (non-prelim): 82 length of query: 501 length of database: 7,911,228 effective HSP length: 102 effective length of query: 399 effective length of database: 7,908,066 effective search space: 3155318334 effective search space used: 3155318334 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 62 (28.5 bits)