TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium transferase [Homo sapiens] (501 letters) Database: H.arabidopsidis/genome.fa 5422 sequences; 67,459,135 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABWE01001454.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 107 5e-48 gb|ABWE01000154.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 60 4e-11 gb|ABWE01001560.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 31 5.1 >gb|ABWE01001454.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont60.4, whole genome shotgun sequence Length = 13529 Score = 107 bits (267), Expect(4) = 5e-48 Identities = 46/81 (56%), Positives = 62/81 (76%) Frame = -3 Query: 249 HIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISK 308 H++NNAYGVQ+S C+H I R+GR+DA VQSLDKNF+VPVGGAI+ + + ++K Sbjct: 13005 HVINNAYGVQASACVHQIDLAMRIGRVDAVVQSLDKNFLVPVGGAIVCSTSSEIVDSVAK 12826 Query: 309 MYPGRASASPSLDVLITLLSL 329 YPGRASA+P+LD IT+L + Sbjct: 12825 FYPGRASATPALDFFITMLQM 12763 Score = 59.3 bits (142), Expect = 1e-08 Identities = 24/44 (54%), Positives = 33/44 (75%) Frame = -2 Query: 150 LCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVL 193 L L L+ RP A+Y+IWPR+DQKSCFK+++ AG P+V+ N L Sbjct: 13402 LVLLQLKKTRPAARYVIWPRVDQKSCFKAILAAGLTPLVLANCL 13271 Score = 55.8 bits (133), Expect(4) = 5e-48 Identities = 48/154 (31%), Positives = 72/154 (46%), Gaps = 13/154 (8%) Frame = -1 Query: 107 KAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVPMATGMSLTLCFLT--------LRHK 158 KAAGSSLL ++ N+L D+++LAG+ V + G T LRH Sbjct: 13529 KAAGSSLLVQLANALAKDVLRLAGLRAVQAHWCCL*RLGCRYAGAAATEKDKTRGALRHL 13350 Query: 159 RPKAKYIIWP-----RIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELG 213 I++ R D S K + + G + L+TDL + A + + G Sbjct: 13349 ASGRPEILFQGHFGGRTDATSAGK-LFSRGR*SL-------WGVLKTDLVGMAALMDQYG 13194 Query: 214 PDCILCIHSTTSCFAPRVPDRLEELAVICANYDI 247 + IL + STTSCFAPR D ++E+A +C +I Sbjct: 13193 SESILAVSSTTSCFAPRAYDAVKEIAQLCQERNI 13092 Score = 46.6 bits (109), Expect(4) = 5e-48 Identities = 32/104 (30%), Positives = 49/104 (47%), Gaps = 29/104 (27%) Frame = -1 Query: 330 GSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNP------------------- 370 G NGY++LL+ERK + +Y+ +++ L+ E +L N Sbjct: 12677 GKNGYRRLLEERKHLAAYMREKLEVLAAEEGEHVLKVSTNEVGTIELKLRQCWFSDRLTF 12498 Query: 371 ----ISLAMTLKTL------DEHRDKAVTQLGSMLFTRQVSGAR 404 IS AMTL T + + + +T LG+MLF+R V GAR Sbjct: 12497 VRA*ISYAMTLATFCSEVEDRQEKSRRLTLLGAMLFSRGVCGAR 12366 Score = 42.7 bits (99), Expect(4) = 5e-48 Identities = 19/63 (30%), Positives = 37/63 (58%) Frame = -3 Query: 404 RVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRK 463 RVV + ++GY F F +H + +P AY+ A +GM+ +VD+ + +L + + RK Sbjct: 12297 RVVSCLDSKCIAGYEFDSFGAHYDEFPVAYVTFACVLGMQKTEVDMLMTKLRKTIHDWRK 12118 Query: 464 ERS 466 +++ Sbjct: 12117 KQN 12109 >gb|ABWE01000154.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont60.5, whole genome shotgun sequence Length = 69632 Score = 59.7 bits (143), Expect(2) = 4e-11 Identities = 29/72 (40%), Positives = 45/72 (62%) Frame = -2 Query: 1 MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAI 60 M+ ++ A E+LV YV+QG A RS L+ LL + P++GWD++++E+ L L+ Sbjct: 277 MDSKTAALAEQLVPATYVQQGLAAIRSRHKLVSTLLAQRCLPDDGWDDTSVEMLLQTLSA 98 Query: 61 MDSNNFLGNCGV 72 MDSNNF G+ Sbjct: 97 MDSNNFRAAYGL 62 Score = 27.7 bits (60), Expect(2) = 4e-11 Identities = 13/19 (68%), Positives = 14/19 (73%) Frame = -1 Query: 73 GEREGRVASALVARRHYRF 91 GER RV S LVARRH+ F Sbjct: 62 GER*ARVFSTLVARRHFGF 6 >gb|ABWE01001560.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont259.3, whole genome shotgun sequence Length = 11960 Score = 30.8 bits (68), Expect = 5.1 Identities = 25/84 (29%), Positives = 37/84 (44%), Gaps = 5/84 (5%) Frame = +2 Query: 89 YRFIHGIGRSGDISAVQPKAAGSSLLNKITN-----SLVLDIIKLAGVHTVANCFVVPMA 143 YRF +GR G ++ + G + L +TN L ++I V ANC + + Sbjct: 8834 YRF-PALGRDG--RRLKRGSKGRTRLPPLTNLTGPNELAHELILAGPVQVAANCEICFLT 9004 Query: 144 TGMSLTLCFLTLRHKRPKAKYIIW 167 T MS C +TL H +IW Sbjct: 9005 TTMSTNRCVVTLIHDTQS*ITMIW 9076 Database: H.arabidopsidis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 67,459,135 Number of sequences in database: 5422 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 37,039,385 Number of Sequences: 5422 Number of extensions: 541719 Number of successful extensions: 2769 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 318 Number of HSP's successfully gapped in prelim test: 132 Number of HSP's that attempted gapping in prelim test: 2331 Number of HSP's gapped (non-prelim): 826 length of query: 501 length of database: 22,486,378 effective HSP length: 110 effective length of query: 391 effective length of database: 21,889,958 effective search space: 8558973578 effective search space used: 8558973578 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 66 (30.0 bits)