TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium transferase [Homo sapiens] (501 letters) Database: E.siliculosus/genome.fa 35 sequences; 195,934,119 total letters Searching...................................done Score E Sequences producing significant alignments: (bits) Value emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genom... 85 7e-16 emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genom... 32 8.7 >emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG17 Length = 5807719 Score = 85.1 bits (209), Expect = 7e-16 Identities = 41/68 (60%), Positives = 53/68 (77%) Frame = +2 Query: 78 RVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANC 137 +V S LVARR++R HG+GRSGDI+ VQPKAAGSSLL K+T++L D ++LAG+ C Sbjct: 98831 QVYSGLVARRNFRLSHGVGRSGDIAEVQPKAAGSSLLAKLTHALAKDALRLAGLGNARAC 99010 Query: 138 FVVPMATG 145 V+PMATG Sbjct: 99011 LVLPMATG 99034 Score = 68.2 bits (165), Expect = 8e-11 Identities = 34/76 (44%), Positives = 47/76 (61%) Frame = +3 Query: 392 GSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFI 451 GSMLFTR VSG RVVP + V G F G+ + YP YL AA A G+ +++D F+ Sbjct: 103224 GSMLFTRCVSGTRVVPREQHKCVGGIDFNGYGASVTGYPHDYLTAACAAGLSTEELDEFL 103403 Query: 452 KRLDRCLKAVRKERSK 467 RLD+ L+ + ER++ Sbjct: 103404 IRLDKALRKAKAERAE 103451 Score = 67.4 bits (163), Expect = 1e-10 Identities = 28/55 (50%), Positives = 41/55 (74%) Frame = +3 Query: 140 VPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLE 194 V + + MSL L F LR +P AK I+WPR+DQKSC K+++ AGF P+V+EN+++ Sbjct: 99360 VLLQSSMSLALTFAALRAAKPAAKTIVWPRMDQKSCLKAIVAAGFTPIVVENLIQ 99524 Score = 58.2 bits (139), Expect = 9e-08 Identities = 26/53 (49%), Positives = 35/53 (66%) Frame = +2 Query: 27 SHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRV 79 S+ + LL + P GWD++ +E L E+++MDSNNF N GVGEREGRV Sbjct: 98240 SYNTQVTALLSNRRLPTRGWDDAQIEYLLTEISLMDSNNFSENVGVGEREGRV 98398 Score = 50.4 bits (119), Expect = 2e-05 Identities = 25/54 (46%), Positives = 39/54 (72%) Frame = +1 Query: 272 VGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLIT 325 VGR+DA VQS DKNF+VPVGGA++ G + +FI++ +++ P S + VL++ Sbjct: 101563 VGRVDAVVQSTDKNFLVPVGGAVVCGPDATFIEQ-ARVTPEEISRRQTPRVLLS 101721 Score = 49.7 bits (117), Expect = 3e-05 Identities = 20/41 (48%), Positives = 30/41 (73%) Frame = +2 Query: 195 GDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRL 235 GD + TD+ V A V+ G + +LC+ +TTSCFAPR+PD++ Sbjct: 99773 GDAVATDVDGVRAAVESCGAENVLCVVTTTSCFAPRLPDKV 99895 Score = 47.8 bits (112), Expect = 1e-04 Identities = 20/40 (50%), Positives = 30/40 (75%) Frame = +1 Query: 305 EISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEM 344 ++ K Y GRAS+ P LD+ ITLLS+G G+++LL ER+ + Sbjct: 101944 QVGKSYAGRASSGPCLDLFITLLSMGQEGWRRLLAERETL 102063 Score = 41.2 bits (95), Expect = 0.011 Identities = 17/38 (44%), Positives = 27/38 (71%) Frame = +1 Query: 231 VPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQ 268 +P +++E+A ICA + H++NNAYGVQ SK L+ + Sbjct: 101155 LPLKVDEVARICAASGVGHVINNAYGVQCSKTCRLVNR 101268 >emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, unassigned sequences LGUn Length = 58155991 Score = 31.6 bits (70), Expect = 8.7 Identities = 18/49 (36%), Positives = 27/49 (55%), Gaps = 2/49 (4%) Frame = -2 Query: 244 NYDIPHIVNNAYGVQSSK--CMHLIQQGARVGRIDAFVQSLDKNFMVPV 290 NY +PH + ++ VQ K C+ L + + G AF QS KNF+ P+ Sbjct: 15689736 NYPLPHALTGSFAVQYMKGACLPL-RCTKKKGHASAFRQSRAKNFLRPI 15689593 Database: E.siliculosus/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 195,934,119 Number of sequences in database: 35 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 114,667,393 Number of Sequences: 35 Number of extensions: 1765219 Number of successful extensions: 9577 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 9351 Number of HSP's gapped (non-prelim): 2295 length of query: 501 length of database: 65,311,373 effective HSP length: 117 effective length of query: 384 effective length of database: 65,307,278 effective search space: 25077994752 effective search space used: 25077994752 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 70 (31.6 bits)